diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config
index ec9300ef4f..2650572c93 100644
--- a/nf_core/pipeline-template/nextflow.config
+++ b/nf_core/pipeline-template/nextflow.config
@@ -107,7 +107,6 @@ profiles {
     }
     docker {
         docker.enabled         = true
-        docker.registry        = 'quay.io'
         docker.userEmulation   = true
         conda.enabled          = false
         singularity.enabled    = false
@@ -131,7 +130,6 @@ profiles {
     }
     podman {
         podman.enabled         = true
-        podman.registry        = 'quay.io'
         conda.enabled          = false
         docker.enabled         = false
         singularity.enabled    = false
@@ -175,6 +173,12 @@ profiles {
     test_full { includeConfig 'conf/test_full.config' }
 }
 
+// Set default registry for Docker and Podman independent of -profile
+// Will not be used unless Docker / Podman are enabled
+// Set to your registry if you have a mirror of containers
+docker.registry = 'quay.io'
+podman.registry = 'quay.io'
+
 {% if igenomes %}
 // Load igenomes.config if required
 if (!params.igenomes_ignore) {