diff --git a/lib/assert.js b/lib/assert.js
index a83dba770e0344..d307582d1fde7d 100644
--- a/lib/assert.js
+++ b/lib/assert.js
@@ -269,7 +269,7 @@ function getErrMessage(message, fn) {
   const call = err.stack[0];
 
   const filename = call.getFileName();
-  const line = call.getLineNumber() - 1;
+  let line = call.getLineNumber() - 1;
   let column = call.getColumnNumber() - 1;
   let identifier;
   let code;
@@ -289,6 +289,9 @@ function getErrMessage(message, fn) {
       return message;
     }
     code = String(fn);
+    // For functions created with the Function constructor, V8 does not count
+    // the lines containing the function header.
+    line += 2;
     identifier = `${code}${line}${column}`;
   }