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391c0294-07f1-4856-b592-428bd44055ca.xml
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391c0294-07f1-4856-b592-428bd44055ca.xml
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<gmd:CI_Address>
<gmd:electronicMailAddress><gco:CharacterString>info@eidc.ac.uk</gco:CharacterString></gmd:electronicMailAddress>
</gmd:CI_Address>
</gmd:address>
</gmd:CI_Contact>
</gmd:contactInfo>
<gmd:role>
<gmd:CI_RoleCode codeList="https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#CI_RoleCode" codeListValue="distributor">distributor</gmd:CI_RoleCode>
</gmd:role>
</gmd:CI_ResponsibleParty>
</gmd:distributorContact>
</gmd:MD_Distributor>
</gmd:distributor>
<gmd:transferOptions>
<gmd:MD_DigitalTransferOptions>
<gmd:onLine>
<gmd:CI_OnlineResource>
<gmd:linkage><gmd:URL>https://data-package.ceh.ac.uk/data/391c0294-07f1-4856-b592-428bd44055ca</gmd:URL></gmd:linkage>
<gmd:name><gco:CharacterString>Download the data</gco:CharacterString></gmd:name>
<gmd:description><gco:CharacterString>Download a copy of this data</gco:CharacterString></gmd:description>
<gmd:function>
<gmd:CI_OnLineFunctionCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnLineFunctionCode>
</gmd:function>
</gmd:CI_OnlineResource>
</gmd:onLine>
</gmd:MD_DigitalTransferOptions>
</gmd:transferOptions>
<gmd:transferOptions>
<gmd:MD_DigitalTransferOptions>
<gmd:onLine>
<gmd:CI_OnlineResource>
<gmd:linkage><gmd:URL>https://data-package.ceh.ac.uk/sd/391c0294-07f1-4856-b592-428bd44055ca.zip</gmd:URL></gmd:linkage>
<gmd:name><gco:CharacterString>Supporting information</gco:CharacterString></gmd:name>
<gmd:description><gco:CharacterString>Supporting information available to assist in re-use of this dataset</gco:CharacterString></gmd:description>
<gmd:function>
<gmd:CI_OnLineFunctionCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information">information</gmd:CI_OnLineFunctionCode>
</gmd:function>
</gmd:CI_OnlineResource>
</gmd:onLine>
</gmd:MD_DigitalTransferOptions>
</gmd:transferOptions>
</gmd:MD_Distribution>
</gmd:distributionInfo>
<gmd:dataQualityInfo>
<gmd:DQ_DataQuality>
<gmd:scope>
<gmd:DQ_Scope>
<gmd:level>
<gmd:MD_ScopeCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
</gmd:level>
<gmd:levelDescription>
<gmd:MD_ScopeDescription>
<gmd:other><gco:CharacterString>dataset</gco:CharacterString></gmd:other>
</gmd:MD_ScopeDescription>
</gmd:levelDescription>
</gmd:DQ_Scope>
</gmd:scope>
<gmd:report>
<gmd:DQ_DomainConsistency>
<gmd:result>
<gmd:DQ_ConformanceResult>
<gmd:specification>
<gmd:CI_Citation>
<gmd:title>
<gco:CharacterString>Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services</gco:CharacterString>
</gmd:title>
<gmd:date>
<gmd:CI_Date>
<gmd:date>
<gco:Date>2010-12-08</gco:Date>
</gmd:date>
<gmd:dateType>
<gmd:CI_DateTypeCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/gmxCodelists.xml#CI_DateTypeCode" codeListValue="publication"/>
</gmd:dateType>
</gmd:CI_Date>
</gmd:date>
</gmd:CI_Citation>
</gmd:specification>
<gmd:explanation gco:nilReason="inapplicable"/>
<gmd:pass gco:nilReason="unknown"/>
</gmd:DQ_ConformanceResult>
</gmd:result>
</gmd:DQ_DomainConsistency>
</gmd:report>
<gmd:lineage>
<gmd:LI_Lineage>
<gmd:statement><gco:CharacterString>Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs'). Soil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific). Quantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018. Extracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012. All results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.</gco:CharacterString></gmd:statement>
</gmd:LI_Lineage>
</gmd:lineage>
</gmd:DQ_DataQuality>
</gmd:dataQualityInfo>
</gmd:MD_Metadata>