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bielefeld_pub_1610436.xml
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bielefeld_pub_1610436.xml
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<titleInfo>
<title>Identification of binding mechanisms in single molecule-DNA complexes</title>
</titleInfo>
<name type="personal">
<namePart type="given">Rainer</namePart>
<namePart type="family">Eckel</namePart>
<role>
<roleTerm type="code" authority="marcrelator">aut</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Robert</namePart>
<namePart type="family">Ros</namePart>
<role>
<roleTerm type="code" authority="marcrelator">aut</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Alexandra</namePart>
<namePart type="family">Ros</namePart>
<role>
<roleTerm type="code" authority="marcrelator">aut</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">S.D.</namePart>
<namePart type="family">Wilking</namePart>
<role>
<roleTerm type="code" authority="marcrelator">aut</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Norbert</namePart>
<namePart type="family">Sewald</namePart>
<nameIdentifier type="orcid" typeURI="http://orcid.org">0000-0002-0309-2655</nameIdentifier>
<role>
<roleTerm type="code" authority="marcrelator">aut</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Dario</namePart>
<namePart type="family">Anselmetti</namePart>
<nameIdentifier type="orcid" typeURI="http://orcid.org">0000-0002-8720-4369</nameIdentifier>
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<typeOfResource>text</typeOfResource>
<genre authority="dini">article</genre>
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<dateIssued encoding="w3cdtf">2003</dateIssued>
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<language>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
<abstract lang="eng">Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules in the presence of different DNA-binding agents are identified using atomic force microscope single molecule force spectroscopy. We investigated the binding of poly(dG-dC) dsDNA with the minor groove binder distamycin A, two supposed major groove binders, an alpha-helical and a 3(10)-helical peptide, the intercalants daunomycin, ethidium bromide and YO, and the bis-intercalant YOYO. Characteristic mechanical fingerprints in the overstretching behavior of the studied single DNA-ligand complexes were observed allowing the distinction between different binding modes. Docking of ligands to the minor or major groove of DNA has the effect that the intramolecular B-S transition remains visible as a distinct plateau in the force-extension trace. By contrast, intercalation of small molecules into the double helix is characterized by the vanishing of the B-S plateau. These findings lead to the conclusion that atomic force microscope force spectroscopy can be regarded as a single molecule biosensor and is a potent tool for the characterization of binding motives of small ligands to DNA.</abstract>
<subject>
</subject>
<classification authority="ddc">572</classification>
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<titleInfo>
<title>Biophysical journal</title>
</titleInfo>
<part>
<detail type="volume">
<number>85</number>
</detail>
<detail type="issue">
<number>3</number>
</detail>
<extent unit="page">
<start>1968</start>
<end>1973</end>
</extent>
</part>
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<identifier type="issn">0006-3495</identifier>
<identifier type="MEDLINE">12944309</identifier>
<identifier type="ISI">000185009900056</identifier>
<identifier type="urn">urn:nbn:de:0070-pub-16104364</identifier>
<identifier type="doi">10.1016/S0006-3495(03)74624-4</identifier>
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<mets:FLocat xlink:href="https://pub.uni-bielefeld.de/download/1610436/2311133/Biophysj_2003.pdf" LOCTYPE="URL"/>
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