We read every piece of feedback, and take your input very seriously.
To see all available qualifiers, see our documentation.
Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.
By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.
Already on GitHub? Sign in to your account
根据说明,pFind.spectra文件表头中Specificity列应为:0: non-specific; 1: C端特异性酶切; 2: N端特异性酶切; 3:两端都特异性酶切。我们发现我们样品得到的结果文件pFind.spectra似乎不符合上述取值规律:样品elastase酶切(酶切位点是AVSGLI的C端),搜库参数为Non-Specific,在pFind_Filtered.spectra、pFind.protein文件中所有肽段的Specificity均为0(non-specific),但pFind.spectra文件共27677个PSM中,却显示存在Specificity为1的肽段252个(例如:A.YRRFYGPV.-、R.RFYGPV.-、K.EQPPQPLFSFDFGA.S;其中两个间隔符“.”之间的为匹配的PSM,前后各为DataBase中蛋白被匹配段前后的氨基酸),Specificity为2的肽段289个(例如:G.APGVPGPA.G、-.MGPQGAR.R、M.GEGPVGEPPPL.Q)。尽管这些肽段全部因为q value>0.01而被筛去,以Non-Specific为搜库参数时是否应该所有肽段的Specificity都为0?
The text was updated successfully, but these errors were encountered:
No branches or pull requests
根据说明,pFind.spectra文件表头中Specificity列应为:0: non-specific; 1: C端特异性酶切; 2: N端特异性酶切; 3:两端都特异性酶切。我们发现我们样品得到的结果文件pFind.spectra似乎不符合上述取值规律:样品elastase酶切(酶切位点是AVSGLI的C端),搜库参数为Non-Specific,在pFind_Filtered.spectra、pFind.protein文件中所有肽段的Specificity均为0(non-specific),但pFind.spectra文件共27677个PSM中,却显示存在Specificity为1的肽段252个(例如:A.YRRFYGPV.-、R.RFYGPV.-、K.EQPPQPLFSFDFGA.S;其中两个间隔符“.”之间的为匹配的PSM,前后各为DataBase中蛋白被匹配段前后的氨基酸),Specificity为2的肽段289个(例如:G.APGVPGPA.G、-.MGPQGAR.R、M.GEGPVGEPPPL.Q)。尽管这些肽段全部因为q value>0.01而被筛去,以Non-Specific为搜库参数时是否应该所有肽段的Specificity都为0?
The text was updated successfully, but these errors were encountered: