diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 04d69b1..396d99b 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -22,7 +22,7 @@ repos:
     rev: "1.2.0"
     hooks:
       - id: pyproject-fmt
-        additional_dependencies: ["tox>=4.10"]
+        additional_dependencies: ["tox>=4.11.3"]
   - repo: https://github.com/pre-commit/mirrors-prettier
     rev: "v3.0.3"
     hooks:
diff --git a/README.md b/README.md
index c9aa52b..85dea1e 100644
--- a/README.md
+++ b/README.md
@@ -20,6 +20,25 @@ pip install pytest-env
 
 ## Usage
 
+### Native form in `pyproject.toml`
+
+```toml
+[tool.pytest_env]
+HOME = "~/tmp"
+RUN_ENV = 1
+TRANSFORMED = {value = "{USER}/alpha", transform: true}
+SKIP_IF_SET = {value = "on", skip_if_set: true}
+```
+
+The `tool.pytest_env` tables keys are the environment variables keys to set. The right hands-ide of the assigment:
+
+- if an inline table you can set options via the `transform` or `skip_if_set` keys, while the `value` key holds the
+  value to set (or transform before setting). For transformation the variables you can use is other environment
+  variable,
+- otherwise the value to set for the environment variable to set (casted to a string).
+
+### Via pytest configurations
+
 In your pytest.ini file add a key value pair with `env` as the key and the environment variables as a line separated
 list of `KEY=VALUE` entries. The defined variables will be added to the environment before any tests are run:
 
diff --git a/pyproject.toml b/pyproject.toml
index b136d19..4a8ec77 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -35,11 +35,13 @@ dynamic = [
   "version",
 ]
 dependencies = [
-  "pytest>=7.4",
+  "pytest>=7.4.2",
+  'tomli>=2.0.1; python_version < "3.11"',
 ]
 optional-dependencies.test = [
-  "coverage>=7.3",
-  "pytest-mock>=3.11.1",
+  "covdefaults>=2.3",
+  "coverage>=7.3.2",
+  "pytest-mock>=3.12",
 ]
 urls.Homepage = "https://github.com/pytest-dev/pytest-env"
 urls.Source = "https://github.com/pytest-dev/pytest-env"
@@ -85,7 +87,8 @@ run.source = ["pytest_env", "tests"]
 run.dynamic_context = "test_function"
 run.branch = true
 run.parallel = true
-report.fail_under = 92
+run.plugins = ["covdefaults"]
+report.fail_under = 100
 report.show_missing = true
 html.show_contexts = true
 html.skip_covered = false
@@ -99,6 +102,6 @@ paths.source = [
 ]
 
 [tool.mypy]
-python_version = "3.10"
+python_version = "3.11"
 show_error_codes = true
 strict = true
diff --git a/src/pytest_env/plugin.py b/src/pytest_env/plugin.py
index be7a5e5..f242cef 100644
--- a/src/pytest_env/plugin.py
+++ b/src/pytest_env/plugin.py
@@ -2,9 +2,18 @@
 from __future__ import annotations
 
 import os
+import sys
+from dataclasses import dataclass
+from itertools import chain
+from typing import Iterator
 
 import pytest
 
+if sys.version_info >= (3, 11):  # pragma: >=3.11 cover
+    import tomllib
+else:  # pragma: <3.11 cover
+    import tomli as tomllib
+
 
 def pytest_addoption(parser: pytest.Parser) -> None:
     """Add section to configuration files."""
@@ -12,6 +21,14 @@ def pytest_addoption(parser: pytest.Parser) -> None:
     parser.addini("env", type="linelist", help=help_msg, default=[])
 
 
+@dataclass
+class Entry:
+    key: str
+    value: str
+    transform: bool
+    skip_if_set: bool
+
+
 @pytest.hookimpl(tryfirst=True)
 def pytest_load_initial_conftests(
     args: list[str],  # noqa: ARG001
@@ -19,19 +36,41 @@ def pytest_load_initial_conftests(
     parser: pytest.Parser,  # noqa: ARG001
 ) -> None:
     """Load environment variables from configuration files."""
-    for line in early_config.getini("env"):
-        # INI lines e.g. D:R:NAME=VAL has two flags (R and D), NAME key, and VAL value
-        parts = line.partition("=")
-        ini_key_parts = parts[0].split(":")
-        flags = {k.strip().upper() for k in ini_key_parts[:-1]}
-        # R: is a way to designate whether to use raw value -> perform no transformation of the value
-        transform = "R" not in flags
-        # D: is a way to mark the value to be set only if it does not exist yet
-        skip_if_set = "D" in flags
-        key = ini_key_parts[-1].strip()
-        value = parts[2].strip()
-
-        if skip_if_set and key in os.environ:
+    for entry in _load_values(early_config):
+        if entry.skip_if_set and entry.key in os.environ:
             continue
         # transformation -> replace environment variables, e.g. TEST_DIR={USER}/repo_test_dir.
-        os.environ[key] = value.format(**os.environ) if transform else value
+        os.environ[entry.key] = entry.value.format(**os.environ) if entry.transform else entry.value
+
+
+def _load_values(early_config: pytest.Config) -> Iterator[Entry]:
+    has_toml_conf = False
+    for path in chain.from_iterable([[early_config.rootpath], early_config.rootpath.parents]):
+        toml_file = path / "pyproject.toml"
+        if toml_file.exists():
+            with toml_file.open("rb") as file_handler:
+                config = tomllib.load(file_handler)
+                if "tool" in config and "pytest_env" in config["tool"]:
+                    has_toml_conf = True
+                    for key, entry in config["tool"]["pytest_env"].get("env", {}).items():
+                        if isinstance(entry, dict):
+                            value = str(entry["value"])
+                            transform, skip_if_set = bool(entry.get("transform")), bool(entry.get("skip_if_set"))
+                        else:
+                            value, transform, skip_if_set = str(entry), False, False
+                        yield Entry(key, value, transform, skip_if_set)
+            break
+
+    if not has_toml_conf:
+        for line in early_config.getini("env"):
+            # INI lines e.g. D:R:NAME=VAL has two flags (R and D), NAME key, and VAL value
+            parts = line.partition("=")
+            ini_key_parts = parts[0].split(":")
+            flags = {k.strip().upper() for k in ini_key_parts[:-1]}
+            # R: is a way to designate whether to use raw value -> perform no transformation of the value
+            transform = "R" not in flags
+            # D: is a way to mark the value to be set only if it does not exist yet
+            skip_if_set = "D" in flags
+            key = ini_key_parts[-1].strip()
+            value = parts[2].strip()
+            yield Entry(key, value, transform, skip_if_set)
diff --git a/tests/test_env.py b/tests/test_env.py
index a409b60..d9b32b6 100644
--- a/tests/test_env.py
+++ b/tests/test_env.py
@@ -92,7 +92,7 @@
         ),
     ],
 )
-def test_env(
+def test_env_via_pytest(
     testdir: pytest.Testdir,
     env: dict[str, str],
     ini: str,
@@ -116,3 +116,84 @@ def test_env(
         result = testdir.runpytest()
 
     result.assert_outcomes(passed=1)
+
+
+@pytest.mark.parametrize(
+    ("env", "toml", "ini", "expected_env"),
+    [
+        pytest.param(
+            {},
+            '[tool.pytest.ini_options]\nenv = ["MAGIC=toml", "MAGIC_2=toml2"]',
+            "[pytest]\nenv = MAGIC=ini\n MAGIC_2=ini2",
+            {"MAGIC": "ini", "MAGIC_2": "ini2"},
+            id="ini over toml ini_options",
+        ),
+        pytest.param(
+            {},
+            '[tool.pytest.ini_options]\nenv = ["MAGIC=toml", "MAGIC_2=toml2"]',
+            "",
+            {"MAGIC": "toml", "MAGIC_2": "toml2"},
+            id="toml via ini_options",
+        ),
+        pytest.param(
+            {},
+            '[tool.pytest_env.env]\nMAGIC = 1\nMAGIC_2 = "toml2"',
+            "",
+            {"MAGIC": "1", "MAGIC_2": "toml2"},
+            id="toml native",
+        ),
+        pytest.param(
+            {},
+            '[tool.pytest_env.env]\nMAGIC = 1\nMAGIC_2 = "toml2"',
+            "[pytest]\nenv = MAGIC=ini\n MAGIC_2=ini2",
+            {"MAGIC": "1", "MAGIC_2": "toml2"},
+            id="toml native over ini",
+        ),
+        pytest.param(
+            {},
+            '[tool.pytest_env.env]\nMAGIC = {value = "toml", "transform"= true, "skip_if_set" = true}',
+            "",
+            {"MAGIC": "toml"},
+            id="toml inline table",
+        ),
+    ],
+)
+def test_env_via_toml(
+    testdir: pytest.Testdir,
+    env: dict[str, str],
+    toml: str,
+    ini: str,
+    expected_env: dict[str, str],
+    request: pytest.FixtureRequest,
+) -> None:
+    tmp_dir = Path(str(testdir.tmpdir))
+    test_name = re.sub(r"\W|^(?=\d)", "_", request.node.callspec.id).lower()
+    Path(str(tmp_dir / f"test_{test_name}.py")).symlink_to(Path(__file__).parent / "template.py")
+    if ini:
+        (tmp_dir / "pytest.ini").write_text(ini, encoding="utf-8")
+    (tmp_dir / "pyproject.toml").write_text(toml, encoding="utf-8")
+
+    new_env = {
+        **env,
+        "_TEST_ENV": repr(expected_env),
+        "PYTEST_DISABLE_PLUGIN_AUTOLOAD": "1",
+        "PYTEST_PLUGINS": "pytest_env.plugin",
+    }
+
+    # monkeypatch persists env variables across parametrized tests, therefore using mock.patch.dict
+    with mock.patch.dict(os.environ, new_env, clear=True):
+        result = testdir.runpytest()
+
+    result.assert_outcomes(passed=1)
+
+
+def test_env_via_toml_bad(testdir: pytest.Testdir) -> None:
+    toml_file = Path(str(testdir.tmpdir)) / "pyproject.toml"
+    toml_file.write_text("bad toml", encoding="utf-8")
+
+    result = testdir.runpytest()
+    assert result.ret == 4
+    assert result.errlines == [
+        f"ERROR: {toml_file}: Expected '=' after a key in a key/value pair (at line 1, column 5)",
+        "",
+    ]
diff --git a/tox.ini b/tox.ini
index e743352..a75a872 100644
--- a/tox.ini
+++ b/tox.ini
@@ -43,7 +43,7 @@ commands =
 [testenv:type]
 description = run type check on code base
 deps =
-    mypy==1.5.1
+    mypy==1.6.1
 set_env =
     {tty:MYPY_FORCE_COLOR = 1}
 commands =
@@ -54,7 +54,7 @@ commands =
 description = check that the long description is valid
 skip_install = true
 deps =
-    build[virtualenv]>=0.10
+    build[virtualenv]>=1.0.3
     twine>=4.0.2
 change_dir = {toxinidir}
 commands =