forked from CostaLab/CrossTalkeR
-
Notifications
You must be signed in to change notification settings - Fork 0
/
tutorial.html
621 lines (576 loc) · 78.7 KB
/
tutorial.html
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
<!doctype html>
<html>
<head>
<meta charset='UTF-8'><meta name='viewport' content='width=device-width initial-scale=1'>
<title>CellPhoneDB Tutorial</title><link href='https://fonts.loli.net/css?family=Open+Sans:400italic,700italic,700,400&subset=latin,latin-ext' rel='stylesheet' type='text/css' /><style type='text/css'>html {overflow-x: initial !important;}:root { --bg-color:#ffffff; --text-color:#333333; --select-text-bg-color:#B5D6FC; --select-text-font-color:auto; --monospace:"Lucida Console",Consolas,"Courier",monospace; }
html { font-size: 14px; background-color: var(--bg-color); color: var(--text-color); font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; -webkit-font-smoothing: antialiased; }
body { margin: 0px; padding: 0px; height: auto; bottom: 0px; top: 0px; left: 0px; right: 0px; font-size: 1rem; line-height: 1.42857; overflow-x: hidden; background: inherit; tab-size: 4; }
iframe { margin: auto; }
a.url { word-break: break-all; }
a:active, a:hover { outline: 0px; }
.in-text-selection, ::selection { text-shadow: none; background: var(--select-text-bg-color); color: var(--select-text-font-color); }
#write { margin: 0px auto; height: auto; width: inherit; word-break: normal; overflow-wrap: break-word; position: relative; white-space: normal; overflow-x: visible; padding-top: 40px; }
#write.first-line-indent p { text-indent: 2em; }
#write.first-line-indent li p, #write.first-line-indent p * { text-indent: 0px; }
#write.first-line-indent li { margin-left: 2em; }
.for-image #write { padding-left: 8px; padding-right: 8px; }
body.typora-export { padding-left: 30px; padding-right: 30px; }
.typora-export .footnote-line, .typora-export li, .typora-export p { white-space: pre-wrap; }
.typora-export .task-list-item input { pointer-events: none; }
@media screen and (max-width: 500px) {
body.typora-export { padding-left: 0px; padding-right: 0px; }
#write { padding-left: 20px; padding-right: 20px; }
.CodeMirror-sizer { margin-left: 0px !important; }
.CodeMirror-gutters { display: none !important; }
}
#write li > figure:last-child { margin-bottom: 0.5rem; }
#write ol, #write ul { position: relative; }
img { max-width: 100%; vertical-align: middle; image-orientation: from-image; }
button, input, select, textarea { color: inherit; font: inherit; }
input[type="checkbox"], input[type="radio"] { line-height: normal; padding: 0px; }
*, ::after, ::before { box-sizing: border-box; }
#write h1, #write h2, #write h3, #write h4, #write h5, #write h6, #write p, #write pre { width: inherit; }
#write h1, #write h2, #write h3, #write h4, #write h5, #write h6, #write p { position: relative; }
p { line-height: inherit; }
h1, h2, h3, h4, h5, h6 { break-after: avoid-page; break-inside: avoid; orphans: 4; }
p { orphans: 4; }
h1 { font-size: 2rem; }
h2 { font-size: 1.8rem; }
h3 { font-size: 1.6rem; }
h4 { font-size: 1.4rem; }
h5 { font-size: 1.2rem; }
h6 { font-size: 1rem; }
.md-math-block, .md-rawblock, h1, h2, h3, h4, h5, h6, p { margin-top: 1rem; margin-bottom: 1rem; }
.hidden { display: none; }
.md-blockmeta { color: rgb(204, 204, 204); font-weight: 700; font-style: italic; }
a { cursor: pointer; }
sup.md-footnote { padding: 2px 4px; background-color: rgba(238, 238, 238, 0.7); color: rgb(85, 85, 85); border-radius: 4px; cursor: pointer; }
sup.md-footnote a, sup.md-footnote a:hover { color: inherit; text-transform: inherit; text-decoration: inherit; }
#write input[type="checkbox"] { cursor: pointer; width: inherit; height: inherit; }
figure { overflow-x: auto; margin: 1.2em 0px; max-width: calc(100% + 16px); padding: 0px; }
figure > table { margin: 0px; }
tr { break-inside: avoid; break-after: auto; }
thead { display: table-header-group; }
table { border-collapse: collapse; border-spacing: 0px; width: 100%; overflow: auto; break-inside: auto; text-align: left; }
table.md-table td { min-width: 32px; }
.CodeMirror-gutters { border-right: 0px; background-color: inherit; }
.CodeMirror-linenumber { user-select: none; }
.CodeMirror { text-align: left; }
.CodeMirror-placeholder { opacity: 0.3; }
.CodeMirror pre { padding: 0px 4px; }
.CodeMirror-lines { padding: 0px; }
div.hr:focus { cursor: none; }
#write pre { white-space: pre-wrap; }
#write.fences-no-line-wrapping pre { white-space: pre; }
#write pre.ty-contain-cm { white-space: normal; }
.CodeMirror-gutters { margin-right: 4px; }
.md-fences { font-size: 0.9rem; display: block; break-inside: avoid; text-align: left; overflow: visible; white-space: pre; background: inherit; position: relative !important; }
.md-diagram-panel { width: 100%; margin-top: 10px; text-align: center; padding-top: 0px; padding-bottom: 8px; overflow-x: auto; }
#write .md-fences.mock-cm { white-space: pre-wrap; }
.md-fences.md-fences-with-lineno { padding-left: 0px; }
#write.fences-no-line-wrapping .md-fences.mock-cm { white-space: pre; overflow-x: auto; }
.md-fences.mock-cm.md-fences-with-lineno { padding-left: 8px; }
.CodeMirror-line, twitterwidget { break-inside: avoid; }
.footnotes { opacity: 0.8; font-size: 0.9rem; margin-top: 1em; margin-bottom: 1em; }
.footnotes + .footnotes { margin-top: 0px; }
.md-reset { margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: top; background: 0px 0px; text-decoration: none; text-shadow: none; float: none; position: static; width: auto; height: auto; white-space: nowrap; cursor: inherit; -webkit-tap-highlight-color: transparent; line-height: normal; font-weight: 400; text-align: left; box-sizing: content-box; direction: ltr; }
li div { padding-top: 0px; }
blockquote { margin: 1rem 0px; }
li .mathjax-block, li p { margin: 0.5rem 0px; }
li { margin: 0px; position: relative; }
blockquote > :last-child { margin-bottom: 0px; }
blockquote > :first-child, li > :first-child { margin-top: 0px; }
.footnotes-area { color: rgb(136, 136, 136); margin-top: 0.714rem; padding-bottom: 0.143rem; white-space: normal; }
#write .footnote-line { white-space: pre-wrap; }
@media print {
body, html { border: 1px solid transparent; height: 99%; break-after: avoid; break-before: avoid; font-variant-ligatures: no-common-ligatures; }
#write { margin-top: 0px; padding-top: 0px; border-color: transparent !important; }
.typora-export * { -webkit-print-color-adjust: exact; }
html.blink-to-pdf { font-size: 13px; }
.typora-export #write { break-after: avoid; }
.typora-export #write::after { height: 0px; }
.is-mac table { break-inside: avoid; }
}
.footnote-line { margin-top: 0.714em; font-size: 0.7em; }
a img, img a { cursor: pointer; }
pre.md-meta-block { font-size: 0.8rem; min-height: 0.8rem; white-space: pre-wrap; background: rgb(204, 204, 204); display: block; overflow-x: hidden; }
p > .md-image:only-child:not(.md-img-error) img, p > img:only-child { display: block; margin: auto; }
#write.first-line-indent p > .md-image:only-child:not(.md-img-error) img { left: -2em; position: relative; }
p > .md-image:only-child { display: inline-block; width: 100%; }
#write .MathJax_Display { margin: 0.8em 0px 0px; }
.md-math-block { width: 100%; }
.md-math-block:not(:empty)::after { display: none; }
[contenteditable="true"]:active, [contenteditable="true"]:focus, [contenteditable="false"]:active, [contenteditable="false"]:focus { outline: 0px; box-shadow: none; }
.md-task-list-item { position: relative; list-style-type: none; }
.task-list-item.md-task-list-item { padding-left: 0px; }
.md-task-list-item > input { position: absolute; top: 0px; left: 0px; margin-left: -1.2em; margin-top: calc(1em - 10px); border: none; }
.math { font-size: 1rem; }
.md-toc { min-height: 3.58rem; position: relative; font-size: 0.9rem; border-radius: 10px; }
.md-toc-content { position: relative; margin-left: 0px; }
.md-toc-content::after, .md-toc::after { display: none; }
.md-toc-item { display: block; color: rgb(65, 131, 196); }
.md-toc-item a { text-decoration: none; }
.md-toc-inner:hover { text-decoration: underline; }
.md-toc-inner { display: inline-block; cursor: pointer; }
.md-toc-h1 .md-toc-inner { margin-left: 0px; font-weight: 700; }
.md-toc-h2 .md-toc-inner { margin-left: 2em; }
.md-toc-h3 .md-toc-inner { margin-left: 4em; }
.md-toc-h4 .md-toc-inner { margin-left: 6em; }
.md-toc-h5 .md-toc-inner { margin-left: 8em; }
.md-toc-h6 .md-toc-inner { margin-left: 10em; }
@media screen and (max-width: 48em) {
.md-toc-h3 .md-toc-inner { margin-left: 3.5em; }
.md-toc-h4 .md-toc-inner { margin-left: 5em; }
.md-toc-h5 .md-toc-inner { margin-left: 6.5em; }
.md-toc-h6 .md-toc-inner { margin-left: 8em; }
}
a.md-toc-inner { font-size: inherit; font-style: inherit; font-weight: inherit; line-height: inherit; }
.footnote-line a:not(.reversefootnote) { color: inherit; }
.md-attr { display: none; }
.md-fn-count::after { content: "."; }
code, pre, samp, tt { font-family: var(--monospace); }
kbd { margin: 0px 0.1em; padding: 0.1em 0.6em; font-size: 0.8em; color: rgb(36, 39, 41); background: rgb(255, 255, 255); border: 1px solid rgb(173, 179, 185); border-radius: 3px; box-shadow: rgba(12, 13, 14, 0.2) 0px 1px 0px, rgb(255, 255, 255) 0px 0px 0px 2px inset; white-space: nowrap; vertical-align: middle; }
.md-comment { color: rgb(162, 127, 3); opacity: 0.8; font-family: var(--monospace); }
code { text-align: left; vertical-align: initial; }
a.md-print-anchor { white-space: pre !important; border-width: initial !important; border-style: none !important; border-color: initial !important; display: inline-block !important; position: absolute !important; width: 1px !important; right: 0px !important; outline: 0px !important; background: 0px 0px !important; text-decoration: initial !important; text-shadow: initial !important; }
.md-inline-math .MathJax_SVG .noError { display: none !important; }
.html-for-mac .inline-math-svg .MathJax_SVG { vertical-align: 0.2px; }
.md-math-block .MathJax_SVG_Display { text-align: center; margin: 0px; position: relative; text-indent: 0px; max-width: none; max-height: none; min-height: 0px; min-width: 100%; width: auto; overflow-y: hidden; display: block !important; }
.MathJax_SVG_Display, .md-inline-math .MathJax_SVG_Display { width: auto; margin: inherit; display: inline-block !important; }
.MathJax_SVG .MJX-monospace { font-family: var(--monospace); }
.MathJax_SVG .MJX-sans-serif { font-family: sans-serif; }
.MathJax_SVG { display: inline; font-style: normal; font-weight: 400; line-height: normal; zoom: 90%; text-indent: 0px; text-align: left; text-transform: none; letter-spacing: normal; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; padding: 0px; margin: 0px; }
.MathJax_SVG * { transition: none 0s ease 0s; }
.MathJax_SVG_Display svg { vertical-align: middle !important; margin-bottom: 0px !important; margin-top: 0px !important; }
.os-windows.monocolor-emoji .md-emoji { font-family: "Segoe UI Symbol", sans-serif; }
.md-diagram-panel > svg { max-width: 100%; }
[lang="flow"] svg, [lang="mermaid"] svg { max-width: 100%; height: auto; }
[lang="mermaid"] .node text { font-size: 1rem; }
table tr th { border-bottom: 0px; }
video { max-width: 100%; display: block; margin: 0px auto; }
iframe { max-width: 100%; width: 100%; border: none; }
.highlight td, .highlight tr { border: 0px; }
svg[id^="mermaidChart"] { line-height: 1em; }
mark { background: rgb(255, 255, 0); color: rgb(0, 0, 0); }
.md-html-inline .md-plain, .md-html-inline strong, mark .md-inline-math, mark strong { color: inherit; }
mark .md-meta { color: rgb(0, 0, 0); opacity: 0.3 !important; }
@media print {
.typora-export h1, .typora-export h2, .typora-export h3, .typora-export h4, .typora-export h5, .typora-export h6 { break-inside: avoid; }
}
.CodeMirror { height: auto; }
.CodeMirror.cm-s-inner { background: inherit; }
.CodeMirror-scroll { overflow: auto hidden; z-index: 3; }
.CodeMirror-gutter-filler, .CodeMirror-scrollbar-filler { background-color: rgb(255, 255, 255); }
.CodeMirror-gutters { border-right: 1px solid rgb(221, 221, 221); background: inherit; white-space: nowrap; }
.CodeMirror-linenumber { padding: 0px 3px 0px 5px; text-align: right; color: rgb(153, 153, 153); }
.cm-s-inner .cm-keyword { color: rgb(119, 0, 136); }
.cm-s-inner .cm-atom, .cm-s-inner.cm-atom { color: rgb(34, 17, 153); }
.cm-s-inner .cm-number { color: rgb(17, 102, 68); }
.cm-s-inner .cm-def { color: rgb(0, 0, 255); }
.cm-s-inner .cm-variable { color: rgb(0, 0, 0); }
.cm-s-inner .cm-variable-2 { color: rgb(0, 85, 170); }
.cm-s-inner .cm-variable-3 { color: rgb(0, 136, 85); }
.cm-s-inner .cm-string { color: rgb(170, 17, 17); }
.cm-s-inner .cm-property { color: rgb(0, 0, 0); }
.cm-s-inner .cm-operator { color: rgb(152, 26, 26); }
.cm-s-inner .cm-comment, .cm-s-inner.cm-comment { color: rgb(170, 85, 0); }
.cm-s-inner .cm-string-2 { color: rgb(255, 85, 0); }
.cm-s-inner .cm-meta { color: rgb(85, 85, 85); }
.cm-s-inner .cm-qualifier { color: rgb(85, 85, 85); }
.cm-s-inner .cm-builtin { color: rgb(51, 0, 170); }
.cm-s-inner .cm-bracket { color: rgb(153, 153, 119); }
.cm-s-inner .cm-tag { color: rgb(17, 119, 0); }
.cm-s-inner .cm-attribute { color: rgb(0, 0, 204); }
.cm-s-inner .cm-header, .cm-s-inner.cm-header { color: rgb(0, 0, 255); }
.cm-s-inner .cm-quote, .cm-s-inner.cm-quote { color: rgb(0, 153, 0); }
.cm-s-inner .cm-hr, .cm-s-inner.cm-hr { color: rgb(153, 153, 153); }
.cm-s-inner .cm-link, .cm-s-inner.cm-link { color: rgb(0, 0, 204); }
.cm-negative { color: rgb(221, 68, 68); }
.cm-positive { color: rgb(34, 153, 34); }
.cm-header, .cm-strong { font-weight: 700; }
.cm-del { text-decoration: line-through; }
.cm-em { font-style: italic; }
.cm-link { text-decoration: underline; }
.cm-error { color: red; }
.cm-invalidchar { color: red; }
.cm-constant { color: rgb(38, 139, 210); }
.cm-defined { color: rgb(181, 137, 0); }
div.CodeMirror span.CodeMirror-matchingbracket { color: rgb(0, 255, 0); }
div.CodeMirror span.CodeMirror-nonmatchingbracket { color: rgb(255, 34, 34); }
.cm-s-inner .CodeMirror-activeline-background { background: inherit; }
.CodeMirror { position: relative; overflow: hidden; }
.CodeMirror-scroll { height: 100%; outline: 0px; position: relative; box-sizing: content-box; background: inherit; }
.CodeMirror-sizer { position: relative; }
.CodeMirror-gutter-filler, .CodeMirror-hscrollbar, .CodeMirror-scrollbar-filler, .CodeMirror-vscrollbar { position: absolute; z-index: 6; display: none; }
.CodeMirror-vscrollbar { right: 0px; top: 0px; overflow: hidden; }
.CodeMirror-hscrollbar { bottom: 0px; left: 0px; overflow: hidden; }
.CodeMirror-scrollbar-filler { right: 0px; bottom: 0px; }
.CodeMirror-gutter-filler { left: 0px; bottom: 0px; }
.CodeMirror-gutters { position: absolute; left: 0px; top: 0px; padding-bottom: 30px; z-index: 3; }
.CodeMirror-gutter { white-space: normal; height: 100%; box-sizing: content-box; padding-bottom: 30px; margin-bottom: -32px; display: inline-block; }
.CodeMirror-gutter-wrapper { position: absolute; z-index: 4; background: 0px 0px !important; border: none !important; }
.CodeMirror-gutter-background { position: absolute; top: 0px; bottom: 0px; z-index: 4; }
.CodeMirror-gutter-elt { position: absolute; cursor: default; z-index: 4; }
.CodeMirror-lines { cursor: text; }
.CodeMirror pre { border-radius: 0px; border-width: 0px; background: 0px 0px; font-family: inherit; font-size: inherit; margin: 0px; white-space: pre; overflow-wrap: normal; color: inherit; z-index: 2; position: relative; overflow: visible; }
.CodeMirror-wrap pre { overflow-wrap: break-word; white-space: pre-wrap; word-break: normal; }
.CodeMirror-code pre { border-right: 30px solid transparent; width: fit-content; }
.CodeMirror-wrap .CodeMirror-code pre { border-right: none; width: auto; }
.CodeMirror-linebackground { position: absolute; left: 0px; right: 0px; top: 0px; bottom: 0px; z-index: 0; }
.CodeMirror-linewidget { position: relative; z-index: 2; overflow: auto; }
.CodeMirror-wrap .CodeMirror-scroll { overflow-x: hidden; }
.CodeMirror-measure { position: absolute; width: 100%; height: 0px; overflow: hidden; visibility: hidden; }
.CodeMirror-measure pre { position: static; }
.CodeMirror div.CodeMirror-cursor { position: absolute; visibility: hidden; border-right: none; width: 0px; }
.CodeMirror div.CodeMirror-cursor { visibility: hidden; }
.CodeMirror-focused div.CodeMirror-cursor { visibility: inherit; }
.cm-searching { background: rgba(255, 255, 0, 0.4); }
@media print {
.CodeMirror div.CodeMirror-cursor { visibility: hidden; }
}
:root {
--side-bar-bg-color: #fafafa;
--control-text-color: #777;
}
@include-when-export url(https://fonts.loli.net/css?family=Open+Sans:400italic,700italic,700,400&subset=latin,latin-ext);
/* open-sans-regular - latin-ext_latin */
/* open-sans-italic - latin-ext_latin */
/* open-sans-700 - latin-ext_latin */
/* open-sans-700italic - latin-ext_latin */
html {
font-size: 16px;
}
body {
font-family: "Open Sans","Clear Sans", "Helvetica Neue", Helvetica, Arial, sans-serif;
color: rgb(51, 51, 51);
line-height: 1.6;
}
#write {
max-width: 860px;
margin: 0 auto;
padding: 30px;
padding-bottom: 100px;
}
@media only screen and (min-width: 1400px) {
#write {
max-width: 1024px;
}
}
@media only screen and (min-width: 1800px) {
#write {
max-width: 1200px;
}
}
#write > ul:first-child,
#write > ol:first-child{
margin-top: 30px;
}
a {
color: #4183C4;
}
h1,
h2,
h3,
h4,
h5,
h6 {
position: relative;
margin-top: 1rem;
margin-bottom: 1rem;
font-weight: bold;
line-height: 1.4;
cursor: text;
}
h1:hover a.anchor,
h2:hover a.anchor,
h3:hover a.anchor,
h4:hover a.anchor,
h5:hover a.anchor,
h6:hover a.anchor {
text-decoration: none;
}
h1 tt,
h1 code {
font-size: inherit;
}
h2 tt,
h2 code {
font-size: inherit;
}
h3 tt,
h3 code {
font-size: inherit;
}
h4 tt,
h4 code {
font-size: inherit;
}
h5 tt,
h5 code {
font-size: inherit;
}
h6 tt,
h6 code {
font-size: inherit;
}
h1 {
font-size: 2.25em;
line-height: 1.2;
border-bottom: 1px solid #eee;
}
h2 {
font-size: 1.75em;
line-height: 1.225;
border-bottom: 1px solid #eee;
}
/*@media print {
.typora-export h1,
.typora-export h2 {
border-bottom: none;
padding-bottom: initial;
}
.typora-export h1::after,
.typora-export h2::after {
content: "";
display: block;
height: 100px;
margin-top: -96px;
border-top: 1px solid #eee;
}
}*/
h3 {
font-size: 1.5em;
line-height: 1.43;
}
h4 {
font-size: 1.25em;
}
h5 {
font-size: 1em;
}
h6 {
font-size: 1em;
color: #777;
}
p,
blockquote,
ul,
ol,
dl,
table{
margin: 0.8em 0;
}
li>ol,
li>ul {
margin: 0 0;
}
hr {
height: 2px;
padding: 0;
margin: 16px 0;
background-color: #e7e7e7;
border: 0 none;
overflow: hidden;
box-sizing: content-box;
}
li p.first {
display: inline-block;
}
ul,
ol {
padding-left: 30px;
}
ul:first-child,
ol:first-child {
margin-top: 0;
}
ul:last-child,
ol:last-child {
margin-bottom: 0;
}
blockquote {
border-left: 4px solid #dfe2e5;
padding: 0 15px;
color: #777777;
}
blockquote blockquote {
padding-right: 0;
}
table {
padding: 0;
word-break: initial;
}
table tr {
border-top: 1px solid #dfe2e5;
margin: 0;
padding: 0;
}
table tr:nth-child(2n),
thead {
background-color: #f8f8f8;
}
table tr th {
font-weight: bold;
border: 1px solid #dfe2e5;
border-bottom: 0;
margin: 0;
padding: 6px 13px;
}
table tr td {
border: 1px solid #dfe2e5;
margin: 0;
padding: 6px 13px;
}
table tr th:first-child,
table tr td:first-child {
margin-top: 0;
}
table tr th:last-child,
table tr td:last-child {
margin-bottom: 0;
}
.CodeMirror-lines {
padding-left: 4px;
}
.code-tooltip {
box-shadow: 0 1px 1px 0 rgba(0,28,36,.3);
border-top: 1px solid #eef2f2;
}
.md-fences,
code,
tt {
border: 1px solid #e7eaed;
background-color: #f8f8f8;
border-radius: 3px;
padding: 0;
padding: 2px 4px 0px 4px;
font-size: 0.9em;
}
code {
background-color: #f3f4f4;
padding: 0 2px 0 2px;
}
.md-fences {
margin-bottom: 15px;
margin-top: 15px;
padding-top: 8px;
padding-bottom: 6px;
}
.md-task-list-item > input {
margin-left: -1.3em;
}
@media print {
html {
font-size: 13px;
}
table,
pre {
page-break-inside: avoid;
}
pre {
word-wrap: break-word;
}
}
.md-fences {
background-color: #f8f8f8;
}
#write pre.md-meta-block {
padding: 1rem;
font-size: 85%;
line-height: 1.45;
background-color: #f7f7f7;
border: 0;
border-radius: 3px;
color: #777777;
margin-top: 0 !important;
}
.mathjax-block>.code-tooltip {
bottom: .375rem;
}
.md-mathjax-midline {
background: #fafafa;
}
#write>h3.md-focus:before{
left: -1.5625rem;
top: .375rem;
}
#write>h4.md-focus:before{
left: -1.5625rem;
top: .285714286rem;
}
#write>h5.md-focus:before{
left: -1.5625rem;
top: .285714286rem;
}
#write>h6.md-focus:before{
left: -1.5625rem;
top: .285714286rem;
}
.md-image>.md-meta {
/*border: 1px solid #ddd;*/
border-radius: 3px;
padding: 2px 0px 0px 4px;
font-size: 0.9em;
color: inherit;
}
.md-tag {
color: #a7a7a7;
opacity: 1;
}
.md-toc {
margin-top:20px;
padding-bottom:20px;
}
.sidebar-tabs {
border-bottom: none;
}
#typora-quick-open {
border: 1px solid #ddd;
background-color: #f8f8f8;
}
#typora-quick-open-item {
background-color: #FAFAFA;
border-color: #FEFEFE #e5e5e5 #e5e5e5 #eee;
border-style: solid;
border-width: 1px;
}
/** focus mode */
.on-focus-mode blockquote {
border-left-color: rgba(85, 85, 85, 0.12);
}
header, .context-menu, .megamenu-content, footer{
font-family: "Segoe UI", "Arial", sans-serif;
}
.file-node-content:hover .file-node-icon,
.file-node-content:hover .file-node-open-state{
visibility: visible;
}
.mac-seamless-mode #typora-sidebar {
background-color: #fafafa;
background-color: var(--side-bar-bg-color);
}
.md-lang {
color: #b4654d;
}
.html-for-mac .context-menu {
--item-hover-bg-color: #E6F0FE;
}
#md-notification .btn {
border: 0;
}
.dropdown-menu .divider {
border-color: #e5e5e5;
}
.ty-preferences .window-content {
background-color: #fafafa;
}
.ty-preferences .nav-group-item.active {
color: white;
background: #999;
}
</style>
</head>
<body class='typora-export'>
<div id='write' class=''><h2><a name="cellphonedb-cookbook" class="md-header-anchor"></a><span>CellPhoneDB CookBook</span></h2><p><em><span>by James Nagai</span></em></p><h3><a name="0--installation" class="md-header-anchor"></a><span>0- Installation</span></h3><p> </p><p><code>pip3 install cellphonedb</code></p><p> </p><h3><a name="1---step-extract-data-from-seurat-object" class="md-header-anchor"></a><span>1 - Step Extract data from Seurat Object</span></h3><pre spellcheck="false" class="md-fences md-end-block ty-contain-cm modeLoaded" lang="R" style="break-inside: unset;"><div class="CodeMirror cm-s-inner CodeMirror-wrap" lang="r"><div style="overflow: hidden; position: relative; width: 3px; height: 0px; top: 0px; left: 8px;"><textarea autocorrect="off" autocapitalize="off" spellcheck="false" tabindex="0" style="position: absolute; bottom: -1em; padding: 0px; width: 1000px; height: 1em; outline: none;"></textarea></div><div class="CodeMirror-scrollbar-filler" cm-not-content="true"></div><div class="CodeMirror-gutter-filler" cm-not-content="true"></div><div class="CodeMirror-scroll" tabindex="-1"><div class="CodeMirror-sizer" style="margin-left: 0px; margin-bottom: 0px; border-right-width: 0px; padding-right: 0px; padding-bottom: 0px;"><div style="position: relative; top: 0px;"><div class="CodeMirror-lines" role="presentation"><div role="presentation" style="position: relative; outline: none;"><div class="CodeMirror-measure"><pre>x</pre></div><div class="CodeMirror-measure"></div><div style="position: relative; z-index: 1;"></div><div class="CodeMirror-code" role="presentation" style=""><div class="CodeMirror-activeline" style="position: relative;"><div class="CodeMirror-activeline-background CodeMirror-linebackground"></div><div class="CodeMirror-gutter-background CodeMirror-activeline-gutter" style="left: 0px; width: 0px;"></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">require</span>(<span class="cm-variable">EWCE</span>)</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">require</span>(<span class="cm-variable">tibble</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">require</span>(<span class="cm-variable">biomaRt</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">require</span>(<span class="cm-variable">tidyr</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">require</span>(<span class="cm-variable">dplyr</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment"># Basic function to convert mouse to human gene names</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">data_</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">readRDS</span>(<span class="cm-string">'path_to_data'</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">alldata</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">NormalizeData</span>(<span class="cm-variable">alldata</span>, <span class="cm-variable">normalization.method</span> <span class="cm-operator">=</span> <span class="cm-string">"LogNormalize"</span>, <span class="cm-variable">scale.factor</span> <span class="cm-operator">=</span> <span class="cm-number">10000</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">allgenes</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">rownames</span>(<span class="cm-variable">alldata</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">matrix1</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">as.data.frame</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">assays</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">RNA</span>@<span class="cm-variable">data</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">matrix1</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">matrix1</span>[<span class="cm-variable">rowSums</span>(<span class="cm-variable">matrix1</span>[,<span class="cm-number">2</span><span class="cm-operator">:</span><span class="cm-variable">dim</span>(<span class="cm-variable">matrix1</span>)[<span class="cm-number">2</span>]])<span class="cm-operator">!=</span><span class="cm-number">0</span>,]</span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment">### If you are using a mouse data, then its needed to convert the gene names to human orthologs</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">human</span> <span class="cm-operator">=</span> <span class="cm-variable">useEnsembl</span>(<span class="cm-string">"ensembl"</span>, <span class="cm-variable">dataset</span> <span class="cm-operator">=</span> <span class="cm-string">"hsapiens_gene_ensembl"</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">mouse</span> <span class="cm-operator">=</span> <span class="cm-variable">useEnsembl</span>(<span class="cm-string">"ensembl"</span>, <span class="cm-variable">dataset</span> <span class="cm-operator">=</span> <span class="cm-string">"mmusculus_gene_ensembl"</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">genesV2</span> <span class="cm-operator">=</span> <span class="cm-variable">getLDS</span>(<span class="cm-variable">attributes</span> <span class="cm-operator">=</span> <span class="cm-variable">c</span>(<span class="cm-string">"mgi_symbol"</span>), <span class="cm-variable">filters</span> <span class="cm-operator">=</span> <span class="cm-string">"mgi_symbol"</span>, <span class="cm-variable">values</span> <span class="cm-operator">=</span> <span class="cm-variable">rownames</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">assays</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">RNA</span>@<span class="cm-variable">data</span>) , <span class="cm-variable">mart</span> <span class="cm-operator">=</span> <span class="cm-variable">mouse</span>, <span class="cm-variable">attributesL</span> <span class="cm-operator">=</span> <span class="cm-variable">c</span>(<span class="cm-string">"hgnc_symbol"</span>,<span class="cm-string">"hgnc_id"</span>,<span class="cm-string">'ensembl_gene_id'</span>), <span class="cm-variable">martL</span> <span class="cm-operator">=</span> <span class="cm-variable">human</span>, <span class="cm-variable">uniqueRows</span><span class="cm-operator">=</span><span class="cm-variable">T</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">print</span>(<span class="cm-variable">head</span>(<span class="cm-variable">genesV2</span>))</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">matrix1</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">matrix1</span>[<span class="cm-variable">match</span>(<span class="cm-variable">genesV2</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">MGI.symbol</span>,<span class="cm-variable">rownames</span>(<span class="cm-variable">alldata</span>),<span class="cm-variable">nomatch</span><span class="cm-operator">=</span><span class="cm-variable">F</span>),]</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">matrix1</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">gene</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">genesV2</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">Gene.stable.ID</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment">#rownames(matrix1) <- genesV2$Gene.stable.ID</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment">### Subseting the matrix</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s1</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">grepl</span>(<span class="cm-string">'state1'</span>,<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">cond</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s2</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">grepl</span>(<span class="cm-string">'state2'</span>,<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">cond</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s1</span>[<span class="cm-variable">match</span>(<span class="cm-string">'gene'</span>,<span class="cm-variable">colnames</span>(<span class="cm-variable">matrix1</span>))] <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">TRUE</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s2</span>[<span class="cm-variable">match</span>(<span class="cm-string">'gene'</span>,<span class="cm-variable">colnames</span>(<span class="cm-variable">matrix1</span>))] <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">TRUE</span></span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment">## Checking the dimensions</span></span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">print</span>(<span class="cm-variable">dim</span>(<span class="cm-variable">matrix1</span>[,<span class="cm-variable">s1</span>]))</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">print</span>(<span class="cm-variable">dim</span>(<span class="cm-variable">matrix1</span>[,<span class="cm-variable">s2</span>]))</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment">## If the cluster names are categorical, you will need to convert it to numerical</span></span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">as.factor</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>)</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">print</span>(<span class="cm-variable">levels</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>))</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">levels</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>) <span class="cm-operator cm-arrow"><-</span> <span class="cm-number">1</span><span class="cm-operator">:</span><span class="cm-variable">length</span>(<span class="cm-variable">levels</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>))</span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">print</span>(<span class="cm-number">1</span><span class="cm-operator">:</span><span class="cm-variable">length</span>(<span class="cm-variable">levels</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>)))</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">as.numeric</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">write.table</span>(<span class="cm-variable">matrix1</span>[,<span class="cm-variable">s1</span>], <span class="cm-string">'s1_filtered_hcount.csv'</span>,<span class="cm-variable">row.names</span><span class="cm-operator">=</span><span class="cm-variable">T</span>,<span class="cm-variable">sep</span><span class="cm-operator">=</span><span class="cm-string">','</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">write.table</span>(<span class="cm-variable">matrix1</span>[,<span class="cm-variable">s2</span>], <span class="cm-string">'s2_filtered_hcount.csv'</span>,<span class="cm-variable">row.names</span><span class="cm-operator">=</span><span class="cm-variable">T</span>,<span class="cm-variable">sep</span><span class="cm-operator">=</span><span class="cm-string">','</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">metadata</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">data.frame</span>(<span class="cm-variable">cells</span><span class="cm-operator">=</span><span class="cm-variable">rownames</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span>[<span class="cm-variable">grepl</span>(<span class="cm-string">'state1'</span>,<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">stim</span>),]),<span class="cm-variable">cluster</span><span class="cm-operator">=</span><span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>[<span class="cm-variable">grepl</span>(<span class="cm-string">'state1'</span>,<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">stim</span>)])</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">metadata_s2</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">data.frame</span>(<span class="cm-variable">cells</span><span class="cm-operator">=</span><span class="cm-variable">rownames</span>(<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span>[<span class="cm-operator">!</span><span class="cm-variable">grepl</span>(<span class="cm-string">'state1'</span>,<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">stim</span>),]),<span class="cm-variable">cluster</span><span class="cm-operator">=</span><span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">sub_cell_type</span>[<span class="cm-operator">!</span><span class="cm-variable">grepl</span>(<span class="cm-string">'state1'</span>,<span class="cm-variable">alldata</span>@<span class="cm-variable">meta.data</span><span class="cm-operator cm-dollar">$</span><span class="cm-variable">stim</span>)]) <span class="cm-comment">## Just negate grepl('state1',alldata@meta.data$stim),]</span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">print</span>(<span class="cm-string">'Writing Metadata'</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">write.csv</span>(<span class="cm-variable">metadata</span>, <span class="cm-string">'s1_filtered_meta.csv'</span>, <span class="cm-variable">row.names</span><span class="cm-operator">=</span><span class="cm-variable">FALSE</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">write.csv</span>(<span class="cm-variable">metadata_tac</span>, <span class="cm-string">'s2_filtered_meta.csv'</span>, <span class="cm-variable">row.names</span><span class="cm-operator">=</span><span class="cm-variable">FALSE</span>)<span class="cm-variable-3">```</span></span></pre></div></div></div></div></div></div><div style="position: absolute; height: 0px; width: 1px; border-bottom: 0px solid transparent; top: 1196px;"></div><div class="CodeMirror-gutters" style="display: none; height: 1196px; left: 0px;"></div></div></div></pre><p><strong><span>Note that the gene ID needs to be unique, you will need to use an approach to combine multiple mapped orthologs (sum, max or bitwiseor )</span></strong></p><h3><a name="run-cellphonedb" class="md-header-anchor"></a><span>Run CellPhoneDB</span></h3><p><span>** The parameters list is available at </span><a href='https://github.com/Teichlab/cellphonedb' target='_blank' class='url'>https://github.com/Teichlab/cellphonedb</a></p><h4><a name="ensembl-based-id" class="md-header-anchor"></a><span>Ensembl based ID</span></h4><pre spellcheck="false" class="md-fences md-end-block ty-contain-cm modeLoaded" lang=""><div class="CodeMirror cm-s-inner CodeMirror-wrap" lang=""><div style="overflow: hidden; position: relative; width: 3px; height: 0px; top: 0px; left: 8px;"><textarea autocorrect="off" autocapitalize="off" spellcheck="false" tabindex="0" style="position: absolute; bottom: -1em; padding: 0px; width: 1000px; height: 1em; outline: none;"></textarea></div><div class="CodeMirror-scrollbar-filler" cm-not-content="true"></div><div class="CodeMirror-gutter-filler" cm-not-content="true"></div><div class="CodeMirror-scroll" tabindex="-1"><div class="CodeMirror-sizer" style="margin-left: 0px; margin-bottom: 0px; border-right-width: 0px; padding-right: 0px; padding-bottom: 0px;"><div style="position: relative; top: 0px;"><div class="CodeMirror-lines" role="presentation"><div role="presentation" style="position: relative; outline: none;"><div class="CodeMirror-measure"><pre>x</pre></div><div class="CodeMirror-measure"></div><div style="position: relative; z-index: 1;"></div><div class="CodeMirror-code" role="presentation" style=""><div class="CodeMirror-activeline" style="position: relative;"><div class="CodeMirror-activeline-background CodeMirror-linebackground"></div><div class="CodeMirror-gutter-background CodeMirror-activeline-gutter" style="left: 0px; width: 0px;"></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">#! /bin/bash</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">mkdir s1 s2 # creating the output folders</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">cellphonedb method statistical_analysis s1_filtered_meta.csv s1_filtered_hcount.csv --threads 30 --output-path s1/</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">cellphonedb method statistical_analysis s2_filtered_meta.csv s2_filtered_hcount.csv --threads 30 --output-path s2/</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div></div></div></div></div></div><div style="position: absolute; height: 0px; width: 1px; border-bottom: 0px solid transparent; top: 161px;"></div><div class="CodeMirror-gutters" style="display: none; height: 161px;"></div></div></div></pre><h4><a name="hugo-based-id" class="md-header-anchor"></a><span>HUGO based ID</span></h4><pre spellcheck="false" class="md-fences md-end-block ty-contain-cm modeLoaded" lang=""><div class="CodeMirror cm-s-inner CodeMirror-wrap" lang=""><div style="overflow: hidden; position: relative; width: 3px; height: 0px; top: 0px; left: 8px;"><textarea autocorrect="off" autocapitalize="off" spellcheck="false" tabindex="0" style="position: absolute; bottom: -1em; padding: 0px; width: 1000px; height: 1em; outline: none;"></textarea></div><div class="CodeMirror-scrollbar-filler" cm-not-content="true"></div><div class="CodeMirror-gutter-filler" cm-not-content="true"></div><div class="CodeMirror-scroll" tabindex="-1"><div class="CodeMirror-sizer" style="margin-left: 0px; margin-bottom: 0px; border-right-width: 0px; padding-right: 0px; padding-bottom: 0px;"><div style="position: relative; top: 0px;"><div class="CodeMirror-lines" role="presentation"><div role="presentation" style="position: relative; outline: none;"><div class="CodeMirror-measure"><pre>x</pre></div><div class="CodeMirror-measure"></div><div style="position: relative; z-index: 1;"></div><div class="CodeMirror-code" role="presentation" style=""><div class="CodeMirror-activeline" style="position: relative;"><div class="CodeMirror-activeline-background CodeMirror-linebackground"></div><div class="CodeMirror-gutter-background CodeMirror-activeline-gutter" style="left: 0px; width: 0px;"></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">#! /bin/bash</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">mkdir s1 s2 # creating the output folders</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">cellphonedb method statistical_analysis s1_filtered_meta.csv --counts-data hgnc_symbol s1_filtered_hcount.csv --threads 30 --output-path s1/ </span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;">cellphonedb method statistical_analysis s2_filtered_meta.csv --counts-data hgnc_symbol s2_filtered_hcount.csv --threads 30 --output-path s2/ </span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div></div></div></div></div></div><div style="position: absolute; height: 0px; width: 1px; border-bottom: 0px solid transparent; top: 207px;"></div><div class="CodeMirror-gutters" style="display: none; height: 207px;"></div></div></div></pre><h3><a name="extracting-lr" class="md-header-anchor"></a><span>Extracting LR</span></h3><pre lang="python" class="md-fences md-end-block ty-contain-cm modeLoaded" spellcheck="false" style="break-inside: unset;"><div class="CodeMirror cm-s-inner CodeMirror-wrap" lang="python"><div style="overflow: hidden; position: relative; width: 3px; height: 0px; top: 0px; left: 8px;"><textarea autocorrect="off" autocapitalize="off" spellcheck="false" tabindex="0" style="position: absolute; bottom: -1em; padding: 0px; width: 1000px; height: 1em; outline: none;"></textarea></div><div class="CodeMirror-scrollbar-filler" cm-not-content="true"></div><div class="CodeMirror-gutter-filler" cm-not-content="true"></div><div class="CodeMirror-scroll" tabindex="-1"><div class="CodeMirror-sizer" style="margin-left: 0px; margin-bottom: 0px; border-right-width: 0px; padding-right: 0px; padding-bottom: 0px;"><div style="position: relative; top: 0px;"><div class="CodeMirror-lines" role="presentation"><div role="presentation" style="position: relative; outline: none;"><div class="CodeMirror-measure"><pre>x</pre></div><div class="CodeMirror-measure"></div><div style="position: relative; z-index: 1;"></div><div class="CodeMirror-code" role="presentation" style=""><div class="CodeMirror-activeline" style="position: relative;"><div class="CodeMirror-activeline-background CodeMirror-linebackground"></div><div class="CodeMirror-gutter-background CodeMirror-activeline-gutter" style="left: 0px; width: 0px;"></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-keyword">def</span> <span class="cm-def">correct_lr</span>(<span class="cm-variable">data</span>):</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'''</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-string"> Invert the RL to LR and R1R2 to r2>r1</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-string"> '''</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">import</span> <span class="cm-variable">pandas</span> <span class="cm-keyword">as</span> <span class="cm-variable">pd</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">def</span> <span class="cm-def">swap</span>(<span class="cm-variable">a</span>,<span class="cm-variable">b</span>): <span class="cm-keyword">return</span> <span class="cm-variable">b</span>,<span class="cm-variable">a</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">data</span> = <span class="cm-variable">data</span>.<span class="cm-property">to_dict</span>(<span class="cm-string">'index'</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">for</span> <span class="cm-variable">k</span>,<span class="cm-variable">v</span> <span class="cm-keyword">in</span> <span class="cm-variable">data</span>.<span class="cm-property">items</span>():</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">if</span> <span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_fst'</span>] <span class="cm-keyword">and</span> <span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_scn'</span>]:</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_fst'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_scn'</span>] = <span class="cm-variable">swap</span>(<span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_fst'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_scn'</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">v</span>[<span class="cm-string">'Ligand'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor'</span>] = <span class="cm-variable">swap</span>(<span class="cm-variable">v</span>[<span class="cm-string">'Ligand'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor'</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">v</span>[<span class="cm-string">'Ligand.Cluster'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor.Cluster'</span>] = <span class="cm-variable">swap</span>(<span class="cm-variable">v</span>[<span class="cm-string">'Ligand.Cluster'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor.Cluster'</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">elif</span> <span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_fst'</span>] <span class="cm-keyword">and</span> <span class="cm-keyword">not</span> <span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_scn'</span>]:</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_fst'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_scn'</span>] = <span class="cm-variable">swap</span>(<span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_fst'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'isReceptor_scn'</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">v</span>[<span class="cm-string">'Ligand'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor'</span>] = <span class="cm-variable">swap</span>(<span class="cm-variable">v</span>[<span class="cm-string">'Ligand'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor'</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">v</span>[<span class="cm-string">'Ligand.Cluster'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor.Cluster'</span>] = <span class="cm-variable">swap</span>(<span class="cm-variable">v</span>[<span class="cm-string">'Ligand.Cluster'</span>],<span class="cm-variable">v</span>[<span class="cm-string">'Receptor.Cluster'</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">res_df</span> = <span class="cm-variable">pd</span>.<span class="cm-property">DataFrame</span>.<span class="cm-property">from_dict</span>(<span class="cm-variable">data</span>,<span class="cm-variable">orient</span>=<span class="cm-string">'index'</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">return</span> (<span class="cm-variable">res_df</span>)</span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-keyword">def</span> <span class="cm-def">cpdb2df</span>(<span class="cm-variable">data</span>,<span class="cm-variable">clsmapping</span>):</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">data</span> = <span class="cm-variable">data</span>.<span class="cm-property">fillna</span>(<span class="cm-number">0</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span> = {}</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Ligand'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Receptor'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Ligand.Cluster'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Receptor.Cluster'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'isReceptor_fst'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'isReceptor_scn'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'MeanLR'</span>] = []</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">for</span> <span class="cm-variable">i</span> <span class="cm-keyword">in</span> <span class="cm-builtin">range</span>(<span class="cm-variable">data</span>.<span class="cm-property">shape</span>[<span class="cm-number">0</span>]):</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">pair</span> = <span class="cm-builtin">list</span>(<span class="cm-variable">data</span>[<span class="cm-string">'interacting_pair'</span>])[<span class="cm-variable">i</span>].<span class="cm-property">split</span>(<span class="cm-string">'_'</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">for</span> <span class="cm-variable">j</span> <span class="cm-keyword">in</span> <span class="cm-builtin">range</span>(<span class="cm-variable">data</span>.<span class="cm-property">iloc</span>[:,<span class="cm-number">12</span>:].<span class="cm-property">shape</span>[<span class="cm-number">1</span>]):</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">c_pair</span> = <span class="cm-builtin">list</span>(<span class="cm-variable">data</span>.<span class="cm-property">columns</span>)[<span class="cm-variable">j</span><span class="cm-operator">+</span><span class="cm-number">12</span>].<span class="cm-property">split</span>(<span class="cm-string">'|'</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">if</span> <span class="cm-builtin">int</span>(<span class="cm-variable">data</span>.<span class="cm-property">iloc</span>[<span class="cm-variable">i</span>,<span class="cm-variable">j</span><span class="cm-operator">+</span><span class="cm-number">12</span>]) <span class="cm-operator">!</span>=<span class="cm-number">0.0</span>:</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Ligand'</span>].<span class="cm-property">append</span>(<span class="cm-variable">pair</span>[<span class="cm-number">0</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Receptor'</span>].<span class="cm-property">append</span>(<span class="cm-variable">pair</span>[<span class="cm-number">1</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Ligand.Cluster'</span>].<span class="cm-property">append</span>(<span class="cm-variable">clsmapping</span>[<span class="cm-variable">c_pair</span>[<span class="cm-number">0</span>]])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'Receptor.Cluster'</span>].<span class="cm-property">append</span>(<span class="cm-variable">clsmapping</span>[<span class="cm-variable">c_pair</span>[<span class="cm-number">1</span>]])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'isReceptor_fst'</span>].<span class="cm-property">append</span>(<span class="cm-builtin">list</span>(<span class="cm-variable">data</span>[<span class="cm-string">'receptor_a'</span>])[<span class="cm-variable">i</span>])</span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'isReceptor_scn'</span>].<span class="cm-property">append</span>(<span class="cm-builtin">list</span>(<span class="cm-variable">data</span>[<span class="cm-string">'receptor_b'</span>])[<span class="cm-variable">i</span>])</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">df_data</span>[<span class="cm-string">'MeanLR'</span>].<span class="cm-property">append</span>(<span class="cm-variable">data</span>.<span class="cm-property">iloc</span>[<span class="cm-variable">i</span>,<span class="cm-variable">j</span><span class="cm-operator">+</span><span class="cm-number">12</span>])</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-variable">data_final</span> = <span class="cm-variable">pd</span>.<span class="cm-property">DataFrame</span>.<span class="cm-property">from_dict</span>(<span class="cm-variable">df_data</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-keyword">return</span>(<span class="cm-variable">data_final</span>)</span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s1</span> = <span class="cm-variable">pd</span>.<span class="cm-property">read_csv</span>(<span class="cm-string">'./s1/significant_means.txt'</span>,<span class="cm-variable">sep</span>=<span class="cm-string">'\t'</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s2</span> = <span class="cm-variable">pd</span>.<span class="cm-property">read_csv</span>(<span class="cm-string">'./s2/ significant_means.txt'</span>,<span class="cm-variable">sep</span>=<span class="cm-string">'\t'</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment">#dict with the mapping</span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">num_to_clust</span> = {<span class="cm-string">'1'</span>:<span class="cm-string">'Cluters 1'</span>,</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'2'</span>:<span class="cm-string">'Cluters 2'</span>,</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'3'</span>:<span class="cm-string">'Cluters 3'</span>,</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'4'</span>:<span class="cm-string">'Cluters 4'</span>,</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'5'</span>:<span class="cm-string">'Cluters 5'</span>}</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s1_filtered</span> = <span class="cm-variable">cpdb2df</span>(<span class="cm-variable">s1</span>,<span class="cm-variable">num_to_clust</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s2_filtered</span> = <span class="cm-variable">cpdb2df</span>(<span class="cm-variable">s2</span>,<span class="cm-variable">num_to_clust</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s1_filtered</span> = <span class="cm-variable">correct_lr</span>(<span class="cm-variable">s1_filtered</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s2_filtered</span> = <span class="cm-variable">correct_lr</span>(<span class="cm-variable">s2_filtered</span>)</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s1_filtered</span>.<span class="cm-property">to_csv</span>(<span class="cm-string">'s1_filtered_corrected.csv'</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">s2_filtered</span>.<span class="cm-property">to_csv</span>(<span class="cm-string">'s2_filtered_corrected.csv'</span>)</span></pre></div><div class="" style="position: relative;"><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div></div></div></div></div></div><div style="position: absolute; height: 0px; width: 1px; border-bottom: 0px solid transparent; top: 1357px;"></div><div class="CodeMirror-gutters" style="display: none; height: 1357px;"></div></div></div></pre><h3><a name="crosstalk" class="md-header-anchor"></a><span>CrossTalk </span></h3><pre lang="R" class="md-fences md-end-block ty-contain-cm modeLoaded" spellcheck="false" style="break-inside: unset;"><div class="CodeMirror cm-s-inner CodeMirror-wrap" lang="r"><div style="overflow: hidden; position: relative; width: 3px; height: 0px; top: 0px; left: 8px;"><textarea autocorrect="off" autocapitalize="off" spellcheck="false" tabindex="0" style="position: absolute; bottom: -1em; padding: 0px; width: 1000px; height: 1em; outline: none;"></textarea></div><div class="CodeMirror-scrollbar-filler" cm-not-content="true"></div><div class="CodeMirror-gutter-filler" cm-not-content="true"></div><div class="CodeMirror-scroll" tabindex="-1"><div class="CodeMirror-sizer" style="margin-left: 0px; margin-bottom: 0px; border-right-width: 0px; padding-right: 0px; padding-bottom: 0px;"><div style="position: relative; top: 0px;"><div class="CodeMirror-lines" role="presentation"><div role="presentation" style="position: relative; outline: none;"><div class="CodeMirror-measure"></div><div class="CodeMirror-measure"></div><div style="position: relative; z-index: 1;"></div><div class="CodeMirror-code" role="presentation" style=""><div class="CodeMirror-activeline" style="position: relative;"><div class="CodeMirror-activeline-background CodeMirror-linebackground"></div><div class="CodeMirror-gutter-background CodeMirror-activeline-gutter" style="left: 0px; width: 0px;"></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">library</span>(<span class="cm-string">'LRAnalytics'</span>)</span></pre></div><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment"># the method always consider the first path as control: the multiple control case will be handle soon</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">paths</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">c</span>(<span class="cm-string">'CTR'</span> <span class="cm-operator">=</span> <span class="cm-string">'s1_filtered_corrected.csv'</span>, </span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'EXP'</span> <span class="cm-operator">=</span> <span class="cm-string">'s1_filtered_corrected.csv'</span>,</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"> <span class="cm-string">'EXP1'</span> <span class="cm-operator">=</span> <span class="cm-string">'s1_filtered_corrected.csv'</span>,)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment"># Selected gene list </span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">genes</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">c</span>(<span class="cm-string">'TGFB1'</span>, <span class="cm-string">'PF4'</span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-comment"># Generating the report and the object</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-variable">data</span> <span class="cm-operator cm-arrow"><-</span> <span class="cm-variable">generate_report</span>(<span class="cm-variable">paths</span><span class="cm-operator">=</span><span class="cm-variable">paths</span>, <span class="cm-comment"># paths list</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-variable">selected_genes</span><span class="cm-operator">=</span><span class="cm-variable">genes</span>, <span class="cm-comment"># Selected list</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-variable">output</span><span class="cm-operator">=</span><span class="cm-string">'/home/nagai/Documents/'</span>, <span class="cm-comment"># output path</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-variable">threshold</span> <span class="cm-operator">=</span> <span class="cm-number">0</span>, <span class="cm-comment"># threshold of prune edges 0=keep all</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-variable">out_file</span><span class="cm-operator">=</span><span class="cm-string">'All_Nils.html'</span> <span class="cm-variable">report</span> <span class="cm-variable">name</span></span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span><span class="cm-tab" role="presentation" cm-text=" "> </span>)</span></pre><pre class=" CodeMirror-line " role="presentation"><span role="presentation" style="padding-right: 0.1px;"><span cm-text=""></span></span></pre></div></div></div></div></div><div style="position: absolute; height: 0px; width: 1px; border-bottom: 0px solid transparent; top: 414px;"></div><div class="CodeMirror-gutters" style="display: none; height: 414px;"></div></div></div></pre><p> </p><p> </p></div>
</body>
</html>