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This is not really a major bug, but I still wanted to report some warnings when using the fs.pp.aggregate_flowframes() function:
aggregated_data=fs.pp.aggregate_flowframes(
filenames=fcs_files_list, #A list with all my .fcs file pathsc_total=80000,
)
This results in the following warnings:
``bash
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/flowsom/pp/fcs_functions.py:49): ImplicitModificationWarning: Trying to modify attribute `.obs` of view, initializing view as actual.
f.obs["Original_ID"] = np.array(ids, dtype=np.float32)
[/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/anndata/_core/anndata.py:1754](https://file+.vscode-resource.vscode-cdn.net/Users/lucal/miniconda3/envs/cyto/lib/python3.12/site-packages/anndata/_core/anndata.py:1754): UserWarning: Observation names are not unique. To make them unique, call `.obs_names_make_unique`.
utils.warn_names_duplicates("obs")
```
Despite the warnings, the function seems to work as usual.
Best
The text was updated successfully, but these errors were encountered:
Report
Hello,
This is not really a major bug, but I still wanted to report some warnings when using the
fs.pp.aggregate_flowframes()
function:This results in the following warnings:
Best
The text was updated successfully, but these errors were encountered: