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Error when converting Seurat objects to SingleCellExperiment objects #4593

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qiuli848 opened this issue Jun 9, 2021 · 1 comment
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@qiuli848
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qiuli848 commented Jun 9, 2021

Hi, when I was converting Seurat objects to SingleCellExperiment objects, an error consistently occurred. I also used the pbmc3k Seurat objects as an example, the error remained. The error was reported as follows:

Error in value[3L] :
invalid subscript 'e' in 'altExp(, type="character", ...)':
'RNA' not in 'altExpNames()'

download.file("https://cf.10xgenomics.com/samples/cell-exp/1.1.0/pbmc3k/pbmc3k_filtered_gene_bc_matrices.tar.gz",
              "pbmc3k_filtered_gene_bc_matrices.tar.gz")
untar("pbmc3k_filtered_gene_bc_matrices.tar.gz")

data <- Seurat::Read10X("filtered_gene_bc_matrices/hg19/")
query_seur <- Seurat::CreateSeuratObject(data)
query_sce <- Seurat::as.SingleCellExperiment(query_seur)
@qiuli848 qiuli848 added the bug Something isn't working label Jun 9, 2021
@timoast
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timoast commented Jun 9, 2021

See #4531, #4524

@timoast timoast closed this as completed Jun 9, 2021
@timoast timoast added the duplicate This issue or pull request already exists label Jun 9, 2021
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