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params_default.config
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params {
// input sequence
inseq = "${baseDir}/example-data/PPRFG01_05.fasta"
// reference data -- paths must be absolute
ref_dir = "${baseDir}/example-data/references/plasmodium"
ref_species = "Pf3D7_05"
// output directory, if desired
dist_dir = "${baseDir}/example-output"
// enable/disable parts of the pipeline
run_exonerate = false
do_contiguation = true
run_braker = true
do_circos = true
do_pseudo = true
make_embl = true
use_reference = true
fix_polycistrons = false
truncate_input_headers = true
transfer_tool = "liftoff"
is_fungi = false
use_existing = true
is_softmasked = false
mit_bypass = false
// make submission-compatible EMBL files
embl_ena_submission = false
// naming patterns
GENOME_PREFIX = "PPRFG01"
CHR_PATTERN = "PPRFG01_(%w+)"
MAX_GENE_LENGTH = 20000
MAX_OVERLAP = 5
ABACAS_BIN_CHR = "PPRFG01_0"
alphanumeric_ids = false
// ABACAS min match length and similarity
ABACAS_MATCH_SIZE = 500
ABACAS_MATCH_SIM = 85
// RATT parameters
RATT_TRANSFER_TYPE = 'Species'
// AUGUSTUS parameters
AUGUSTUS_GENEMODEL = 'partial'
AUGUSTUS_HINTS_MAXINTRONLEN = '2000'
AUGUSTUS_SCORE_THRESHOLD = 0.8
// Weight description file
// This is a Lua script that needs to implement a function
// `get_weight(gene, regionmapping)'' and assigns a number to be used as
// weight in finding an optimal chain of genes in overlapping clusters
// Use 'weight_plasmodium.lua' or 'weight_kinetoplastid.lua' for plasmodium-
// or kinetoplastid-specific functions
WEIGHT_FILE = "${baseDir}/data/weight/weight_multiexon_generic.lua"
// covariance models for Infernal
NCRNA_MODELS = "${baseDir}/data/cm/rnas.cm"
// Template for spec check output
SPECK_TEMPLATE = "html"
// Transcript (e.g. RNA-seq) evidence file to use for hints generation
// (GTF as produced by cufflinks/cuffmerge/...)
//TRANSCRIPT_FILE = ""
// EMBL file metadata
EMBL_AUTHORS = "Foo Bar"
EMBL_TITLE = "Baz"
EMBL_PUBLICATION = "Quux"
EMBL_GENOME_TYPE = "XXX"
EMBL_CLASSIFICATION = "XXXX"
EMBL_ORGANISM = "Plasmodium praefalciparum strain G01"
EMBL_PROJ_ACCESSION = "123456"
EMBL_DESCRIPTION = "Foo bar"
// output GAF metadata
TAXON_ID = 5833
DB_ID = "Companion"
}