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denovoAssembly.md

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CLC

  • in-house algorithm
  • short & long reads
  • mixing of paired reads (variable insert size & orientation
  • quality trimming!
  • hybrid data (454 & Illumina)
  • the more data the bigger the k-mer size
  • they say 'optimal parameter selection sometimes requires experimentation'
  • -g option --> use certain reads only in later stages (gap closing, bubble resolving, scaffolding)

XworX assembly

  • Comparison of assemblies from different tools at the end

  • ?? Downsampling of input data -> use max coverage

  • TODO - test with gage data

  • TODO - testing framework