diff --git a/Cargo.toml b/Cargo.toml index 0b70ae9..5e5cfd6 100644 --- a/Cargo.toml +++ b/Cargo.toml @@ -1,6 +1,6 @@ [package] name = "rustody" -version = "1.2.4" +version = "1.2.5" edition = "2021" license-file = "LICENSE" description = "Split BD rapsody fastq files into sample specific fastq files" diff --git a/hamming_dist_Ighm_reads.txt b/hamming_dist_Ighm_reads.txt new file mode 100644 index 0000000..8a28bd2 --- /dev/null +++ b/hamming_dist_Ighm_reads.txt @@ -0,0 +1,615 @@ +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCAGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" 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+"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAGAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGTAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGCGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGGAGA" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGATCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" +"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGATGGAGAGGGAGTGGGAAGG" diff --git a/needleman_Ighm_reads.txt b/needleman_Ighm_reads.txt new file mode 100644 index 0000000..c859a06 --- /dev/null +++ b/needleman_Ighm_reads.txt @@ -0,0 +1,5668 @@ +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATCGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACAATACCTCTCGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACCT" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGAGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGCGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAACAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCCGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACCT" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGCGACCAAGAGATCCTCCCCCAGGGACACTCCCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGGGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACTGCAGAGACCAAGAGATCCTCCCACAGGGTCACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"AAGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACAACCTCTGGGCCTGGGAAAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGCGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCCCAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACAAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTGAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACGGCAGAGACCAAGAGATCCTCCCACAGGGACACTGCCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAGGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACGCTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGTGATCCTCCCACAGGGACACTACCTCTAGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGCCACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGTAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGCGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACGGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGCGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCCGGGATAC" +"AAGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCAGGGCCTGGGATAC" +"ATGACTCTCCGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATA" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGACCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTCCCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCGCAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGCCTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"AAGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACTAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGGCCAAGCGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTGTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTATCTCTGGGCCTGGGAAAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGTCCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGAATCTCAGCATAGAAGGACATCAGAGACCAAGAGATCCTCCCACACGGACACTACCTCTGGGCATGGGATA" +"ATGACTCTCAGCAAGGAAGCCCAGCAGAGACCACGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTTGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGCGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGACTGGTATACC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTGCCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGCCTCTCCGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCACTGGGCCAGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGCCTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGCCCAAGAGATCCTCCCACAGGGACACAACCACTGGGCCAGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGAATCGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGTCCTGCGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGTCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGGTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" 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+"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACGGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"GTGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"GTGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGAAA" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGGCACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCACACAGGGACACTACCTCTAGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGCGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGAAAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGAGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGAATGGAAGGACAGCAGAGAACAAGAGATCCTCACACAGGGACACTACCTCTAGGCCTGGGATA" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACCCGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATA" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACGGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCTAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATGC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACCCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGGCACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCGGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACGGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGGGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAGGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCGGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"TGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATACC" +"ATGACTCTCGGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAACAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGAGACACTACCTCTGGTCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGCGACACTACCTCTGGTCCTGGAATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGCTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGGTCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGCACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATA" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" +"ATGACTCTCAGCATGGAAGGACAGCAGAGACCAAGAGATCCTCCCACAGGGACACTACCTCTGGGCCTGGGATAC" diff --git a/src/analysis.rs b/src/analysis.rs index e40b295..9f65ed1 100644 --- a/src/analysis.rs +++ b/src/analysis.rs @@ -304,6 +304,8 @@ impl Analysis{ return Err(FilterError::Ns); } } + + /* // I have huge problems with reds that contain a lot of ploy A in the end // None of them match using blast - so I think we can savely just dump them. let mut bad = 0; @@ -324,6 +326,7 @@ impl Analysis{ //eprintln!("Sequence rejected due to high polyA content \n{:?}", String::from_utf8_lossy(&read2.seq()) ); return Err(FilterError::PolyA) } + */ Ok(()) } diff --git a/src/bin/map_one_sequence.rs b/src/bin/map_one_sequence.rs index ed3c586..415bd87 100644 --- a/src/bin/map_one_sequence.rs +++ b/src/bin/map_one_sequence.rs @@ -14,9 +14,9 @@ static EMPTY_VEC: Vec = Vec::new(); #[derive(Parser)] #[clap(version = "0.0.3", author = "Stefan L. ")] struct Opts { - /// the input R1 reads file + /// the dna to seach for #[clap(short, long)] - sequence: String, + dna: String, /// the fasta database containing the genes #[clap(short, long)] expression: Option, @@ -135,18 +135,63 @@ fn main() { } } + let mut id = 0; + samples.change_start_id( antibodies.last_count ); + if opts.specie.eq("human") { + // get all the human sample IDs into this. + // GTTGTCAAGATGCTACCGTTCAGAGATTCAAGGGCAGCCGCGTCACGATTGGATACGACTGTTGGACCGG + // b"ATTCAAGGGCAGCCGCGTCACGATTGGATACGACTGTTGGACCGG" + // Seams they have introduced new sample ids, too.... + let sequences = [ b"ATTCAAGGGCAGCCGCGTCACGATTGGATACGACTGTTGGACCGG", b"TGGATGGGATAAGTGCGTGATGGACCGAAGGGACCTCGTGGCCGG", + b"CGGCTCGTGCTGCGTCGTCTCAAGTCCAGAAACTCCGTGTATCCT", b"ATTGGGAGGCTTTCGTACCGCTGCCGCCACCAGGTGATACCCGCT", + b"CTCCCTGGTGTTCAATACCCGATGTGGTGGGCAGAATGTGGCTGG", b"TTACCCGCAGGAAGACGTATACCCCTCGTGCCAGGCGACCAATGC", + b"TGTCTACGTCGGACCGCAAGAAGTGAGTCAGAGGCTGCACGCTGT", b"CCCCACCAGGTTGCTTTGTCGGACGAGCCCGCACAGCGCTAGGAT", + b"GTGATCCGCGCAGGCACACATACCGACTCAGATGGGTTGTCCAGG", b"GCAGCCGGCGTCGTACGAGGCACAGCGGAGACTAGATGAGGCCCC", + b"CGCGTCCAATTTCCGAAGCCCCGCCCTAGGAGTTCCCCTGCGTGC", b"GCCCATTCATTGCACCCGCCAGTGATCGACCCTAGTGGAGCTAAG" ]; + + for seq in sequences{ + //seq.reverse(); + let mut seq_ext = b"GTTGTCAAGATGCTACCGTTCAGAG".to_vec(); + seq_ext.extend_from_slice( seq ); + samples.add_small( &seq_ext, format!("Sample{id}"),EMPTY_VEC.clone() ); + //sample_names.push( format!("Sample{id}") ); + id +=1; + } + } + else if opts.specie.eq("mouse") { + // and the mouse ones + let sequences = [b"AAGAGTCGACTGCCATGTCCCCTCCGCGGGTCCGTGCCCCCCAAG", b"ACCGATTAGGTGCGAGGCGCTATAGTCGTACGTCGTTGCCGTGCC", + b"AGGAGGCCCCGCGTGAGAGTGATCAATCCAGGATACATTCCCGTC", b"TTAACCGAGGCGTGAGTTTGGAGCGTACCGGCTTTGCGCAGGGCT", + b"GGCAAGGTGTCACATTGGGCTACCGCGGGAGGTCGACCAGATCCT", b"GCGGGCACAGCGGCTAGGGTGTTCCGGGTGGACCATGGTTCAGGC", + b"ACCGGAGGCGTGTGTACGTGCGTTTCGAATTCCTGTAAGCCCACC", b"TCGCTGCCGTGCTTCATTGTCGCCGTTCTAACCTCCGATGTCTCG", + b"GCCTACCCGCTATGCTCGTCGGCTGGTTAGAGTTTACTGCACGCC", b"TCCCATTCGAATCACGAGGCCGGGTGCGTTCTCCTATGCAATCCC", + b"GGTTGGCTCAGAGGCCCCAGGCTGCGGACGTCGTCGGACTCGCGT", b"CTGGGTGCCTGGTCGGGTTACGTCGGCCCTCGGGTCGCGAAGGTC"]; + + for seq in sequences{ + //seq.reverse(); + //let mut seq_ext = b"GTTGTCAAGATGCTACCGTTCAGAG".to_vec(); + //seq_ext.extend_from_slice( seq ); + //samples.add_small( &seq_ext, format!("Sample{id}"),EMPTY_VEC.clone() ); + samples.add_small( &seq.to_vec(), format!("Sample{id}"),EMPTY_VEC.clone() ); + //sample_names.push( format!("Sample{id}") ); + id +=1; + } + } else { + println!("Sorry, but I have no primers for species {}", &opts.specie); + std::process::exit(1) + } // to understand the mapping a little better I now need the index written to a file - let _ = genes.write_index_txt( format!("{}/genes.index.txt", &opts.outpath) ); - let _ = antibodies.write_index_txt( format!("{}/antibodies.index.txt", &opts.outpath) ); - let _ = samples.write_index_txt( format!("{}/samples.index.txt", &opts.outpath) ); + let _ = genes.write_index_txt( format!("{}/genes_index", &opts.outpath) ); + let _ = antibodies.write_index_txt( format!("{}/antibodies_index", &opts.outpath) ); + let _ = samples.write_index_txt( format!("{}/samples_index", &opts.outpath) ); - eprintln!("Ill check this sequence: '{}'", &opts.sequence ); + eprintln!("Ill check this dna: '{}'", &opts.dna ); let mut collected:HashMap::= HashMap::new(); - let mut ok = match antibodies.get( &opts.sequence.as_bytes(), &mut tool ){ + let mut ok = match antibodies.get( &opts.dna.as_bytes(), &mut tool ){ Some(gene_ids) =>{ //eprintln!("gene id {gene_id:?} seq {:?}", String::from_utf8_lossy(&data[i].1) ); //eprintln!("I got an ab id {gene_id}"); @@ -171,7 +216,7 @@ fn main() { } }; if ! ok{ - ok = match samples.get( &opts.sequence.as_bytes(), &mut tool ){ + ok = match samples.get( &opts.dna.as_bytes(), &mut tool ){ Some(gene_ids) =>{ //eprintln!("gene id {gene_id:?} seq {:?}", String::from_utf8_lossy(&data[i].1) ); //eprintln!("I got an ab id {gene_id}"); @@ -198,7 +243,7 @@ fn main() { } if ! ok{ - let _ = match genes.get( &opts.sequence.as_bytes(), &mut tool ){ + let _ = match genes.get( &opts.dna.as_bytes(), &mut tool ){ Some(gene_ids) =>{ //eprintln!("gene id {gene_id:?} seq {:?}", String::from_utf8_lossy(&data[i].1) ); //eprintln!("I got an ab id {gene_id}"); diff --git a/src/bin/quantify_rhapsody.rs b/src/bin/quantify_rhapsody.rs index bcadb04..ec4fbb2 100644 --- a/src/bin/quantify_rhapsody.rs +++ b/src/bin/quantify_rhapsody.rs @@ -23,7 +23,7 @@ use rustody::ofiles::Ofiles; /// You need quite long R1 and R2 reads for this! (>70R1 and >70R2 \[v1\] and 52 bp reads for v2.96 and v2.384) #[derive(Parser)] -#[clap(version = "1.2.4", author = "Stefan L. ")] +#[clap(version = "1.2.5", author = "Stefan L. ")] struct Opts { /// the input R1 reads file #[clap(short, long)] diff --git a/src/bin/quantify_rhapsody_multi.rs b/src/bin/quantify_rhapsody_multi.rs index a09ab6c..c775c6d 100644 --- a/src/bin/quantify_rhapsody_multi.rs +++ b/src/bin/quantify_rhapsody_multi.rs @@ -23,7 +23,7 @@ use num_cpus; /// You need quite long R1 and R2 reads for this! (>70R1 and >70R2 \[v1\] and 52 bp reads for v2.96 and v2.384) #[derive(Parser)] -#[clap(version = "1.2.4", author = "Stefan L. ")] +#[clap(version = "1.2.5", author = "Stefan L. ")] struct Opts { /// the input R1 reads file #[clap(short, long)] diff --git a/src/fast_mapper/fast_mapper.rs b/src/fast_mapper/fast_mapper.rs index b999b0f..260d401 100644 --- a/src/fast_mapper/fast_mapper.rs +++ b/src/fast_mapper/fast_mapper.rs @@ -270,7 +270,7 @@ impl FastMapper{ //println!("I have now {i} mappers for the gene {name}"); if i == 0 { //eprint!("."); - eprintln!("gene {} ({:?}) does not get an entry in the fast_mapper object! - too short? {} bp", &name.as_str(), classes, seq.len() ); + println!("gene {} ({:?}) does not get an entry in the fast_mapper object! - too short? {} bp", &name.as_str(), classes, seq.len() ); } /*else { println!("I added {i} mappper entries for gene {name}"); @@ -339,14 +339,14 @@ impl FastMapper{ } if self.mapper[entries.0 as usize].add( entries.1, (gene_id, 0), classes.clone() ){ - //eprintln!("I have added a sequence! {:#b}+{:?} -> {gene_id} & {classes:?} ",entries.0, entries.1 ); + //println!("I have added a sequence! {:#b}+{} -> {gene_id} & {classes:?} ",entries.0, entries.1 ); self.pos += 1; self.names_count[gene_id] +=1; if i < self.names_count[gene_id]{ i = self.names_count[gene_id]; } }else { - //eprintln!("NOT - I have added a sequence!"); + //println!("NOT added this sequence! {:#b}+{} -> {gene_id} & {classes:?} ",entries.0, entries.1); self.neg +=1 } @@ -357,7 +357,7 @@ impl FastMapper{ //println!("I have now {i} mappers for the gene {name}"); if i == 0 { //eprint!("."); - eprintln!("gene {} ({:?}) does not get an entry in the fast_mapper object! - too short or a duplicate? {} bp", &name.as_str(), classes, seq.len() ); + println!("gene {} ({:?}) does not get an entry in the fast_mapper object! - too short or a duplicate? {} bp", &name.as_str(), classes, seq.len() ); } /*else { println!("I added {i} mappper entries for gene {name}"); @@ -457,15 +457,16 @@ impl FastMapper{ return false } - // let mut report = false; - // if genes.len() > 2{ - // report = true; - // eprintln!("Lots of genes matched here?: {genes:?}"); - // for (gene_id, _level) in genes.keys(){ - // eprint!(" {}", self.names_store[*gene_id] ); - // } - // eprint!("\n"); - // } + /*let mut report = false; + if genes.len() > 2{ + report = true; + eprintln!("Lots of genes matched here?: {genes:?}"); + for (gene_id, _level) in genes.keys(){ + eprint!(" {}", self.names_store[*gene_id] ); + } + eprint!("\n"); + }*/ + let most_matches = genes.values().max().unwrap_or(&0); //eprintln!("I got this as most matches {most_matches}"); @@ -661,6 +662,7 @@ impl FastMapper{ let mut matching_geneids = Vec::< usize>::with_capacity(10); // entries is a Option<(u16, u64, usize) + /*let mut i = 0; let mut small_seq :String = Default::default(); let mut large_seq: String = Default::default();*/ @@ -671,13 +673,18 @@ impl FastMapper{ // the 8bp bit is a match //eprintln!("We are at iteration {i}"); + if &entries.1.1 < &20_u8{ + //will never map as the mapper fails every sequence below 20 bp - too many false positives! + continue + } /*small_seq.clear(); large_seq.clear(); tool.u16_to_str( 8, &entries.0, &mut small_seq); tool.u64_to_str( entries.1.1.into(), &entries.1.0, &mut large_seq); - eprintln!("We are on iteration {i} with seq {:?}-{:?})", small_seq, large_seq); - */ + println!("We are on iteration {i} with seq {}-{} ( {}-{})", small_seq, large_seq, &entries.0, &entries.1); + i +=1;*/ + // if matching_geneids.len() == 1 && i > 3 { // //eprintln!("we have one best gene identified! {}",matching_geneids[0]); @@ -690,19 +697,19 @@ impl FastMapper{ match &self.mapper[entries.0 as usize].get( &entries.1 ){ Some( gene_ids ) => { - //eprintln!("Got some gene ids (one?): {:?}", gene_ids); + //println!("Got some gene ids (one?): {:?}", gene_ids); for name_entry in gene_ids { for gid in &name_entry.data{ match genes.get_mut( &gid) { Some(gene_count) => { - //eprintln!( "Adding to existsing {} with count {gene_count}+1", gid.0); + //println!( "Adding to existsing {} with count {gene_count}+1", gid.0); *gene_count +=1; if *gene_count == 4 && genes.len() ==1 { break 'main; } }, None => { - //eprintln!( "Adding a new gene {} with count 1 here!", gid.0); + //println!( "Adding a new gene {} with count 1 here!", gid.0); genes.insert( gid.clone(), 1); }, }; @@ -712,7 +719,7 @@ impl FastMapper{ }, None => { - //eprintln!("Got one no gene id in the first run:"); + //eprintln!("Got no gene id in the first run get() run -> find() instead:"); match self.mapper[entries.0 as usize].find( &entries.1 ){ Some( gene_ids ) => { //eprintln!("But in the second I got one: {gene_ids:?}"); @@ -735,7 +742,8 @@ impl FastMapper{ } } }, - None => { + None => { + //eprintln!("And none in the find() either."); //no_32bp_match +=1; }, } @@ -756,17 +764,17 @@ impl FastMapper{ return None } - let bad_gene = "Zbtb16"; - let bad = self.get_id( bad_gene.to_string()) ; + /*let bad_gene = "Ighm"; + let bad = self.get_id( bad_gene.to_string()) ;*/ // check if there is only one gene // if self.get_best_gene( &genes, &mut matching_geneids ){ // Cd3e <- keep that to fiond this place back //println!("I have these genes: {genes:?} And am returning: {:?}", matching_geneids); - if matching_geneids[0] == bad { + /*if matching_geneids[0] == bad { println!("read mapping to {} - should not happen here!: {:?}\n{:?}", bad_gene, self.gene_names_for_ids( &matching_geneids ),String::from_utf8_lossy(seq) ); println!("This is our total matching set: {:?}", genes); - } + }*/ return Some( matching_geneids ) } if matching_geneids.len() > 2{ @@ -1080,7 +1088,7 @@ impl FastMapper{ nucl.clear(); self.tool.u64_to_str( 8, &(i as u64), &mut nucl ); if self.mapper[idx].has_data(){ - match write!(ofile.buff1, "\ni: {} {} ", i, nucl ){ + match write!(ofile.buff1, "\ni: {} ({:016b}) {} ", i, i,nucl ){ Ok(_) => (), //Ok(_) => println!("8bp mapper: {:b} binary -> {:?} bytes",i, &i.to_le_bytes() ) , Err(_err) => return Err::<(), &str>("i could not be written"), diff --git a/src/fast_mapper/mapper_entries/mapper_entries.rs b/src/fast_mapper/mapper_entries/mapper_entries.rs index cbd8844..ca0276f 100644 --- a/src/fast_mapper/mapper_entries/mapper_entries.rs +++ b/src/fast_mapper/mapper_entries/mapper_entries.rs @@ -8,6 +8,7 @@ pub struct MapperEntry{ pub map:Vec::<(SecondSeq, NameEntry)>, // the data storage only:usize, hamming_cut: u32, // the bit difference up to which a match between two 32bp regions would still be acceptable. + needleman_wunsch_cut: u32, } impl MapperEntry{ @@ -17,12 +18,11 @@ impl MapperEntry{ all = 4 } let map = Vec::<(SecondSeq, NameEntry)>::with_capacity(all); - let only =0; - let hamming_cut = 2; Self{ map, - only, - hamming_cut, + only :0, + hamming_cut :2, + needleman_wunsch_cut: 25 } } @@ -55,6 +55,7 @@ impl MapperEntry{ for i in 0..self.map.len() { if self.map[i].0.same(&seq) { self.only = 0; + //println!("Here I have a duplicate second seq! {seq:?}"); return self.map[i].1.add( id, classes.clone()) } } @@ -92,41 +93,51 @@ impl MapperEntry{ None } - /* - pub fn get_mut( &mut self,seq:&SecondSeq ) -> Option> { - - for i in 0..self.map.len() { - if &self.map[i].0 == seq { - // if self.map[i].1.data.len() > 1{ - // eprintln!("Ooops - we have a get in more than one gene: {:?}", self.map[i].1.data); - // } - return Some( &mut self.map[i].1 ) - } - } - // now we have an initial match, but no secondary. - None - } - */ /// finds the most likely matching entry in our set of sequences. /// This now matches - if the u64 does not match in total the u8 4bp kmers instead. /// But not continuousely as that would need me to convert them back to string. /// If this becomes necessary it can be added later. pub fn find (&self, seq:&SecondSeq ) -> Option> { let mut ret : Vec::<&NameEntry> = vec![]; + let mut dists : Vec:: = vec![]; + let mut min_dist: u32 = u32::MAX; for i in 0..self.map.len() { //eprintln!("Hamming distance below {} - returning {:?}", self.hamming_cut, self.map[i].1.data ); - if self.map[i].0.fuzzy_match( seq , self.hamming_cut) { + //let dist = self.map[i].0.hamming_distance( seq ); + let dist = self.map[i].0.needleman_wunsch( seq ); + //eprintln!("Distance is {dist}"); + //if dist <= self.hamming_cut { + if dist <= self.needleman_wunsch_cut { //eprintln!( "{seq:?} did match to {:?} should that be right?", self.map[i].0); - ret.push(&self.map[i].1 ) + ret.push( &self.map[i].1 ); + dists.push( dist ); + if dist < min_dist{ + min_dist = dist + } } } - if ret.len() > 0{ - return Some(ret); + return match ret.len() { + 0 =>{ + //eprintln!("I find nothing here!"); + None + } + 1 =>{ + //eprintln!("I find exactly one!"); + Some(ret) + }, + len =>{ + let mut ret2: Vec::<&NameEntry> = vec![]; + for i in 0..len{ + if dists[i] == min_dist{ + ret2.push( ret[i] ) + } + + } + //eprintln!("I found multiple and {:?} with the lowest difference {}",ret2, min_dist ); + Some(ret2) + } } - // so now we could have a frameshift due to a long stretch of a single nucleotide - // if necessary implement that later on. - // For now I think we should just stop here. - return None + } pub fn print(&self) { diff --git a/src/fast_mapper/mapper_entries/name_entries.rs b/src/fast_mapper/mapper_entries/name_entries.rs index 454aa7f..5149239 100644 --- a/src/fast_mapper/mapper_entries/name_entries.rs +++ b/src/fast_mapper/mapper_entries/name_entries.rs @@ -81,7 +81,7 @@ impl NameEntry{ if self.hashes.contains( &hash){ // the combo of gene id, mapping class, [gene tags] has already been added here. - // eprintln!("I alread had this data in my data storage: {:?}, {:?} -> {}: {:?}", tup, classes, hash, self.hashes.clone() ); + //println!("I alread had this data in my data storage: gene id and class{:?}, more classes {:?} -> this combos hash: {}: my hashes: {:?}", tup, classes, hash, self.hashes.clone() ); return false } //eprintln!("Mapper_entries: I add {:?} and {:?} with hash {}", tup, classes, hash); diff --git a/src/fast_mapper/mapper_entries/second_seq.rs b/src/fast_mapper/mapper_entries/second_seq.rs index fc48537..872309b 100644 --- a/src/fast_mapper/mapper_entries/second_seq.rs +++ b/src/fast_mapper/mapper_entries/second_seq.rs @@ -41,7 +41,13 @@ impl PartialEq for SecondSeq { if length < 20 { return false } - mask = (1 << (length as u64) *2 ) - 1; + + if length >= 32 { + mask = u64::MAX; + // Rest of your code using the mask + } else { + mask = (1 << (length as u64) *2 ) - 1; + } //eprintln!("I'll compare {:b} to {:b}", (self.0 & mask) , (other.0 & mask)); (self.0 & mask) == (other.0 & mask) } @@ -58,7 +64,7 @@ impl Hash for SecondSeq { // Implementing Display trait for SecondSeq impl fmt::Display for SecondSeq { fn fmt(&self, f: &mut fmt::Formatter<'_>) -> fmt::Result { - write!(f, "(u64: {:b}, u8: {})", self.0, self.1) + write!(f, "SecondSeq (u64: {:b} or {}, u8: {})", self.0, self.to_string().as_str(), self.1) } } @@ -66,13 +72,39 @@ impl fmt::Display for SecondSeq { impl SecondSeq { + pub fn to_string(&self) -> String { + let mut data = String::new(); + //println!("converting u64 {loc:b} to string with {kmer_size} bp."); + for i in 0..self.1 { + // Use a mask (0b11) to extract the least significant 2 bits + let ch = match (self.0 >> (i * 2)) & 0b11 { + 0b00 => "A", + 0b01 => "C", + 0b10 => "G", + 0b11 => "T", + _ => "N", + }; + data += ch; + + //println!("{ch} and loc {loc:b}"); + } + + //println!("\nMakes sense? {:?}", data); + data + } + fn max3(a: T, b: T, c: T) -> T { max(a, max(b, c)) } - pub fn needleman_wunsch(&self, other: &SecondSeq ) -> i32 { - let rows: usize = (self.1 + 1) as usize; - let cols: usize = (other.1 + 1) as usize; + /// Almost a needleman_wunsch implementation. It just returns the difference from the expected result + /// comparing the sequences in there minimal defined length. Similar to the hamming_distance function. + pub fn needleman_wunsch(&self, other: &SecondSeq ) -> u32 { + + let size = self.min_length(other); + + let rows: usize = size; + let cols: usize = size; let mut matrix = vec![vec![Cell { score: 0, direction: Direction::Diagonal }; cols]; rows]; @@ -115,21 +147,19 @@ impl SecondSeq { } // Uncomment the following lines to print the alignment matrix - for i in 0..rows { + /*for i in 0..rows { for j in 0..cols { print!("{:4} ", matrix[i][j].score); } println!(); - } + }*/ - matrix[rows - 1][cols - 1].score + (size as i32 - matrix[rows - 1][cols - 1].score).abs() as u32 } - /// calculate the base flips between two u64 sequences - /// stops after having detected 4 different bases. - pub fn hamming_distance(self, other: &SecondSeq) -> u32 { - - //let mask:u64; + /// returns the minimal size the two SecondSeq obejcts are defined for + /// Something between 32 and 0 bp. + pub fn min_length( &self, other: &SecondSeq) -> usize { let size:usize; if other.1 > self.1{ size = self.1 as usize; @@ -138,6 +168,15 @@ impl SecondSeq { size = other.1 as usize; //mask = (1 << (other.1 as u64) *2 ) - 1; } + return size + } + + /// calculate the base flips between two u64 sequences + /// stops after having detected 4 different bases. + pub fn hamming_distance(self, other: &SecondSeq) -> u32 { + + //let mask:u64; + let size = self.min_length(other); let mut a: u64; let mut b: u64; @@ -153,6 +192,7 @@ impl SecondSeq { break; } } + //println!("hamming dist was {ret}"); return ret /* @@ -184,15 +224,16 @@ impl SecondSeq { self.0 == other.0 } - pub fn print_second_seq(seq: &SecondSeq) { + pub fn print_second_seq(&self) { println!("Contents of SecondSeq:"); - println!("u64: {:b}", seq.0); - println!("{} sig bp", seq.1); + println!("u64: {:b} or {:?}", self.0, self.to_string() ); + println!("{} sig bp", self.1); } pub fn fuzzy_match(&self, other:&SecondSeq, max_dist:u32 ) -> bool { - return self.hamming_distance( other ) <= max_dist.try_into().unwrap() + //return self.hamming_distance( other ) <= max_dist.try_into().unwrap() + return self.needleman_wunsch( other ) <= max_dist.try_into().unwrap() } pub fn to_le_bytes(&self) -> [u8; std::mem::size_of::() + std::mem::size_of::()] { let mut bytes = [0; std::mem::size_of::() + std::mem::size_of::()]; diff --git a/src/int_to_str.rs b/src/int_to_str.rs index 34e59cd..639b946 100644 --- a/src/int_to_str.rs +++ b/src/int_to_str.rs @@ -115,13 +115,13 @@ impl IntToStr { return Some(false); } } - if self.checker.len() < 2{ - //println!( "kmer is too simple/not enough diff nucs" ); - return Some(false); - } + // if self.checker.len() < 2{ + // //println!( "kmer is too simple/not enough diff nucs" ); + // return Some(false); + // } for value in self.checker.values(){ - if *value as f32 / (to-start) as f32 > 0.7 { + if *value as f32 / (to-start) as f32 == 1.0 { //println!( "sequence from {start} to {to} is too simple/too many nucs same {value} {start} {to}" ); return Some(false); } @@ -274,7 +274,7 @@ impl IntToStr { }; // shift 4 bp }, None => { - if self.shifted < 5{ + if self.shifted < 8{ //println!("I am shifting on bp"); match self.shift(){ Some(_) => { return self.next() }, diff --git a/tests/fast_mapper.rs b/tests/fast_mapper.rs index 25ca4c5..3bca443 100644 --- a/tests/fast_mapper.rs +++ b/tests/fast_mapper.rs @@ -18,23 +18,23 @@ mod tests { - mapper.add( &b"ATCCCATCCTTCATTGTTCGCCTGG".to_vec(), "Gene1".to_string(), EMPTY_VEC.clone() ); - mapper.names4sparse.insert( "Gene1".to_string(), geneid ); + mapper.add( &b"CCAAGAATGGTTCCTGTGTTGTATATTATTTGGTATCTTTTACTTACCTGCTTGAATACTTGAATAAACCATTCACCGGTTTTAATCCTTTTACTTCAAAACTTACACATACTGACCTAC".to_vec(), "Ulk2".to_string(), EMPTY_VEC.clone() ); + mapper.names4sparse.insert( "Ulk2".to_string(), geneid ); //ATCCCATCCTTCATTGTTCGCCTGGA #0 //CGATTACTTCTGTTCCATCGCCCACACCTTTGAACCCTAGGGCTGGGTTGAACATCTTCTGTCTCCTAGGTCTGC #1 //CGATTACTTCTGTTCCATCGCCCACACCTTTGAACCCTAGGGCTGGGTTGAACATCTTCTGTCTCCTAGGTCTGC #2 //........................................................................... - mapper.add( &b"CGATTACTTCTGTTCCATCGCCCACACCTTTGAACCCTAGGGCTGGGTTGAACATCTTCTGTCTCCTAGGTCTGC".to_vec(), - "Gene2".to_string(),EMPTY_VEC.clone() ); + mapper.add( &b"CGAGGCTGTGTATTACTGTGCCGTGGGGCTCCGGAGCCAGGAAAAGAAGAGGAtggagagggagtgggaaggaGAAAAGTCGTATACAGATTTGGGATCTTAGGCTCTGGAGACATTCAG".to_vec(), + "Vpreb1".to_string(),EMPTY_VEC.clone() ); geneid +=1; - mapper.names4sparse.insert( "Gene1".to_string(), geneid ); + mapper.names4sparse.insert( "Vpreb1".to_string(), geneid ); //assert_eq!( mapper.with_data, 51 ); - assert_eq!( mapper.get( b"ATCCCATCCTTCATTGTTCGCCTGGACTCTCAGAAGCACATCGACTTCTCCCTCCGTTCTCCTTATGGCGGCGGC", &mut tool ), Some(vec![0]) ); - assert_eq!( mapper.get( b"CGATTACTTCTGTTCCATCGCCCACACCTTTGAACCCTAGGGCTGGGTTGAACATCTTCTGTCTCCTAGGTCTGC", &mut tool ), Some(vec![1]) ); + assert_eq!( mapper.get( b"ATATTATTTGGTATCTTTTACTTACCTGCTTGAATACTTGAATAAACCATTC", &mut tool ), Some(vec![0]) ); + assert_eq!( mapper.get( b"AAAAGAAGAGGAtggagagggagtgggaaggaGAAAAGTCG", &mut tool ), Some(vec![1]) ); //adding the same sequence as Gene2 with the name Gene3 should not make the next search return None mapper.add( &b"CGATTACTTCTGTTCCATCGCCCACACCTTTGAACCCTAGGGCTGGGTTGAACATCTTCTGTCTCCTAGGTCTGC".to_vec(), "Gene3".to_string(),EMPTY_VEC.clone() ); @@ -130,8 +130,8 @@ mod tests { #[test] fn check_samples() { let mut mapper = FastMapper::new( 32, 10 ); - let sample2 = b"GTTGTCAAGATGCTACCGTTCAGAGGGCAAGGTGTCACATTGGGCTACCGCGGGAAGTCGACCAGATCCTA"; - //let sample = b"GTTGTCAAGATGCTACCGTTCTGAGGGCAAGGTGTCACTTTGGGCTACCGCGGGAAGTCGACCAGATCCTA"; + let sample2 = b"GTTGTCAAGATGCTACCGTTCAGAGGGCAAGGTGTCACATTGGGCTACCGCGGGAAGTCGACCAGATCCTA"; + //let sample_0 = b"AAGAGTCGACTGCCATGTCCCCTCCGCGGGTCCGTGCCCCCCAAG"; let sample_real = b"GTTGTCAAGATGCTACCGTTCAGAGAAGAGTCGACTGCCATGTCCCCTCCGCGGGTCCGTGCCCCCCAAGAAAA"; let sequences = [ b"AAGAGTCGACTGCCATGTCCCCTCCGCGGGTCCGTGCCCCCCAAG", b"ACCGATTAGGTGCGAGGCGCTATAGTCGTACGTCGTTGCCGTGCC", @@ -187,7 +187,7 @@ mod tests { println!("\n"); assert_eq!( mapper.get_strict( sequences[1], &mut tool ), Some(vec![11]) ); println!("\n"); - assert_eq!( mapper.get_strict( sample2, &mut tool ), None ); + assert_eq!( mapper.get( sample2, &mut tool ), None ); println!("\n"); assert_eq!( mapper.get_strict( &sequences[0][7..], &mut tool ), Some(vec![10]) ); println!("\n"); diff --git a/tests/second_seq.rs b/tests/second_seq.rs index 866c7c5..e386b0a 100644 --- a/tests/second_seq.rs +++ b/tests/second_seq.rs @@ -71,5 +71,16 @@ mod tests { let seq7 = SecondSeq(0b101010, 15); assert_eq!( seq1.needleman_wunsch( &seq7 ), 5 ); } + + #[test] + fn test_to_string() { + + let seq = "TGGTATCTTTTACTTACCTGCTTGAATACTTG".to_string(); + let seq_u64 = 13632604658164817131_u64; + let obj = SecondSeq(seq_u64, 32); + + assert_eq!( obj.to_string(), seq ); + + } }