diff --git a/test/built-ins/Uint8Array/prototype/toBase64/omit-padding.js b/test/built-ins/Uint8Array/prototype/toBase64/omit-padding.js
new file mode 100644
index 00000000000..b6326fbef3c
--- /dev/null
+++ b/test/built-ins/Uint8Array/prototype/toBase64/omit-padding.js
@@ -0,0 +1,23 @@
+// Copyright (C) 2024 Kevin Gibbons. All rights reserved.
+// This code is governed by the BSD license found in the LICENSE file.
+/*---
+esid: sec-uint8array.prototype.tobase64
+description: Conversion of Uint8Arrays to base64 strings exercising the omitPadding option
+features: [uint8array-base64, TypedArray]
+---*/
+
+// works with default alphabet
+assert.sameValue((new Uint8Array([199, 239])).toBase64(), "x+8=");
+assert.sameValue((new Uint8Array([199, 239])).toBase64({ omitPadding: false }), "x+8=");
+assert.sameValue((new Uint8Array([199, 239])).toBase64({ omitPadding: true }), "x+8");
+assert.sameValue((new Uint8Array([255])).toBase64({ omitPadding: true }), "/w");
+
+// works with base64url alphabet
+assert.sameValue((new Uint8Array([199, 239])).toBase64({ alphabet: "base64url" }), "x-8=");
+assert.sameValue((new Uint8Array([199, 239])).toBase64({ alphabet: "base64url", omitPadding: false }), "x-8=");
+assert.sameValue((new Uint8Array([199, 239])).toBase64({ alphabet: "base64url", omitPadding: true }), "x-8");
+assert.sameValue((new Uint8Array([255])).toBase64({ alphabet: "base64url", omitPadding: true }), "_w");
+
+// performs ToBoolean on the argument
+assert.sameValue((new Uint8Array([255])).toBase64({ omitPadding: 0 }), "/w==");
+assert.sameValue((new Uint8Array([255])).toBase64({ omitPadding: 1 }), "/w");