diff --git a/docs/pages/x/api/tree-view/tree-item-2.json b/docs/pages/x/api/tree-view/tree-item-2.json
index 00c9508458ca7..bac50963652a1 100644
--- a/docs/pages/x/api/tree-view/tree-item-2.json
+++ b/docs/pages/x/api/tree-view/tree-item-2.json
@@ -93,7 +93,10 @@
       "isGlobal": true
     }
   ],
+  "spread": true,
+  "themeDefaultProps": true,
   "muiName": "MuiTreeItem2",
+  "forwardsRefTo": "HTMLLIElement",
   "filename": "/packages/x-tree-view/src/TreeItem2/TreeItem2.tsx",
   "inheritance": null,
   "demos": "<ul><li><a href=\"/x/react-tree-view/rich-tree-view/customization/\">Rich Tree View - Customization</a></li>\n<li><a href=\"/x/react-tree-view/simple-tree-view/customization/\">Simple Tree View - Customization</a></li>\n<li><a href=\"/x/react-tree-view/tree-item-customization/\">Tree Item Customization</a></li></ul>",
diff --git a/packages/x-tree-view/src/TreeItem/TreeItem.test.tsx b/packages/x-tree-view/src/TreeItem/TreeItem.test.tsx
index 49a9702b6f238..426275dd9f682 100644
--- a/packages/x-tree-view/src/TreeItem/TreeItem.test.tsx
+++ b/packages/x-tree-view/src/TreeItem/TreeItem.test.tsx
@@ -2,47 +2,11 @@ import * as React from 'react';
 import { expect } from 'chai';
 import PropTypes from 'prop-types';
 import { createRenderer } from '@mui/internal-test-utils';
-import { SimpleTreeViewPluginSignatures } from '@mui/x-tree-view/SimpleTreeView/SimpleTreeView.plugins';
 import { TreeItem, treeItemClasses as classes } from '@mui/x-tree-view/TreeItem';
-import { TreeViewContextValue } from '@mui/x-tree-view/internals/TreeViewProvider';
 import { TreeViewContext } from '@mui/x-tree-view/internals/TreeViewProvider/TreeViewContext';
 import { describeConformance } from 'test/utils/describeConformance';
 import { describeTreeView } from 'test/utils/tree-view/describeTreeView';
-
-const TEST_TREE_VIEW_CONTEXT_VALUE: TreeViewContextValue<SimpleTreeViewPluginSignatures> = {
-  instance: {
-    isItemExpandable: () => false,
-    isItemExpanded: () => false,
-    isItemFocused: () => false,
-    isItemSelected: () => false,
-    isItemDisabled: (itemId: string | null): itemId is string => !!itemId,
-    getTreeItemIdAttribute: () => '',
-    mapFirstCharFromJSX: () => () => {},
-    canItemBeTabbed: () => false,
-  } as any,
-  publicAPI: {
-    focusItem: () => {},
-    getItem: () => ({}),
-    setItemExpansion: () => {},
-  },
-  runItemPlugins: () => ({ rootRef: null, contentRef: null }),
-  wrapItem: ({ children }) => children,
-  wrapRoot: ({ children }) => children,
-  disabledItemsFocusable: false,
-  indentationAtItemLevel: false,
-  icons: {
-    slots: {},
-    slotProps: {},
-  },
-  selection: {
-    multiSelect: false,
-    checkboxSelection: false,
-    disableSelection: false,
-  },
-  rootRef: {
-    current: null,
-  },
-};
+import { getFakeContextValue } from 'test/utils/tree-view/fakeContextValue';
 
 describeTreeView<[]>('TreeItem component', ({ render, treeItemComponentName }) => {
   describe('ContentComponent / ContentProps props (TreeItem only)', () => {
@@ -94,17 +58,13 @@ describe('<TreeItem />', () => {
     inheritComponent: 'li',
     wrapMount: (mount) => (item: React.ReactNode) => {
       const wrapper = mount(
-        <TreeViewContext.Provider value={TEST_TREE_VIEW_CONTEXT_VALUE}>
-          {item}
-        </TreeViewContext.Provider>,
+        <TreeViewContext.Provider value={getFakeContextValue()}>{item}</TreeViewContext.Provider>,
       );
       return wrapper.childAt(0);
     },
     render: (item) => {
       return render(
-        <TreeViewContext.Provider value={TEST_TREE_VIEW_CONTEXT_VALUE}>
-          {item}
-        </TreeViewContext.Provider>,
+        <TreeViewContext.Provider value={getFakeContextValue()}>{item}</TreeViewContext.Provider>,
       );
     },
     muiName: 'MuiTreeItem',
diff --git a/packages/x-tree-view/src/TreeItem2/TreeItem2.test.tsx b/packages/x-tree-view/src/TreeItem2/TreeItem2.test.tsx
new file mode 100644
index 0000000000000..6476ec7eb8a94
--- /dev/null
+++ b/packages/x-tree-view/src/TreeItem2/TreeItem2.test.tsx
@@ -0,0 +1,48 @@
+import * as React from 'react';
+import { createRenderer } from '@mui/internal-test-utils';
+import { TreeItem2 } from '@mui/x-tree-view/TreeItem2';
+import { treeItemClasses as classes } from '@mui/x-tree-view/TreeItem';
+import { TreeViewContext } from '@mui/x-tree-view/internals/TreeViewProvider/TreeViewContext';
+import { describeConformance } from 'test/utils/describeConformance';
+import { getFakeContextValue } from 'test/utils/tree-view/fakeContextValue';
+import { describeSlotsConformance } from 'test/utils/describeSlotsConformance';
+
+describe('<TreeItem2 />', () => {
+  const { render } = createRenderer();
+
+  describeConformance(<TreeItem2 itemId="one" label="one" />, () => ({
+    classes,
+    inheritComponent: 'li',
+    wrapMount: (mount) => (item: React.ReactNode) => {
+      const wrapper = mount(
+        <TreeViewContext.Provider value={getFakeContextValue()}>{item}</TreeViewContext.Provider>,
+      );
+      return wrapper.childAt(0);
+    },
+    render: (item) => {
+      return render(
+        <TreeViewContext.Provider value={getFakeContextValue()}>{item}</TreeViewContext.Provider>,
+      );
+    },
+    muiName: 'MuiTreeItem2',
+    refInstanceof: window.HTMLLIElement,
+    skip: ['reactTestRenderer', 'componentProp', 'componentsProp', 'themeVariants'],
+  }));
+
+  describeSlotsConformance({
+    render,
+    getElement: ({ props, slotName }) => (
+      <TreeViewContext.Provider
+        value={getFakeContextValue({ checkboxSelection: slotName === 'checkbox' })}
+      >
+        <TreeItem2 itemId="one" label="one" {...props} />
+      </TreeViewContext.Provider>
+    ),
+    slots: {
+      label: { className: classes.label },
+      iconContainer: { className: classes.iconContainer },
+      content: { className: classes.content },
+      checkbox: { className: classes.checkbox },
+    },
+  });
+});
diff --git a/test/utils/describeSlotsConformance.tsx b/test/utils/describeSlotsConformance.tsx
new file mode 100644
index 0000000000000..ffb2e2da4eea8
--- /dev/null
+++ b/test/utils/describeSlotsConformance.tsx
@@ -0,0 +1,76 @@
+import * as React from 'react';
+import { expect } from 'chai';
+import createDescribe from '@mui/internal-test-utils/createDescribe';
+import { MuiRenderResult } from '@mui/internal-test-utils/createRenderer';
+
+interface DescribeSlotsConformanceParams {
+  getElement: (params: { slotName: string; props: any }) => React.ReactElement<any>;
+  render: (node: React.ReactElement) => MuiRenderResult;
+  slots: { [slotName: string]: { className: string } };
+}
+
+export function innerDescribeSlotsConformance(params: DescribeSlotsConformanceParams) {
+  const { getElement, render, slots } = params;
+
+  Object.keys(slots).forEach((slotName) => {
+    describe(`Slot: ${slotName}`, () => {
+      it('should replace the default slot when defined', () => {
+        const slotConfig = slots[slotName];
+        const response = render(
+          getElement({
+            slotName,
+            props: {
+              slots: {
+                [slotName]: React.forwardRef((props, ref: React.Ref<HTMLDivElement>) => (
+                  <div ref={ref} data-testid="custom-slot" />
+                )),
+              },
+            },
+          }),
+        );
+
+        // Check if the default slot is being rendered
+        expect(response.container.querySelector(`.${slotConfig.className}`)).to.equal(null);
+
+        // Check if the custom slot is being rendered
+        expect(response.getByTestId('custom-slot')).to.not.equal(null);
+      });
+
+      it('should pass props to the default slot', () => {
+        const slotConfig = slots[slotName];
+        const response = render(
+          getElement({
+            slotName,
+            props: {
+              slotProps: {
+                [slotName]: { 'data-testid': 'default-slot', className: 'default-slot' },
+              },
+            },
+          }),
+        );
+
+        const slotElement = response.container.querySelector(`.${slotConfig.className}`);
+
+        // Check if the default slot is being rendered
+        expect(slotElement).not.to.equal(null);
+
+        // Check if the default slot receives the `data-testid`
+        expect(slotElement).to.have.attribute('data-testid', 'default-slot');
+
+        // Check if the custom class is being applied
+        expect(slotElement).to.have.class('default-slot');
+
+        // Make sure that the default class has not been removed
+        expect(slotElement).to.have.class(slotConfig.className);
+      });
+    });
+  });
+}
+
+/**
+ * Test the slots of the component.
+ */
+export const describeSlotsConformance = createDescribe(
+  'Slots conformance',
+  innerDescribeSlotsConformance,
+);
diff --git a/test/utils/tree-view/fakeContextValue.ts b/test/utils/tree-view/fakeContextValue.ts
new file mode 100644
index 0000000000000..5f4e44545102c
--- /dev/null
+++ b/test/utils/tree-view/fakeContextValue.ts
@@ -0,0 +1,42 @@
+import { TreeViewContextValue } from '@mui/x-tree-view/internals/TreeViewProvider';
+import { SimpleTreeViewPluginSignatures } from '@mui/x-tree-view/SimpleTreeView/SimpleTreeView.plugins';
+
+export const getFakeContextValue = (
+  features: { checkboxSelection?: boolean } = {},
+): TreeViewContextValue<SimpleTreeViewPluginSignatures> => ({
+  instance: {
+    isItemExpandable: () => false,
+    isItemExpanded: () => false,
+    isItemFocused: () => false,
+    isItemSelected: () => false,
+    isItemDisabled: (itemId: string | null): itemId is string => !!itemId,
+    getTreeItemIdAttribute: () => '',
+    mapFirstCharFromJSX: () => () => {},
+    canItemBeTabbed: () => false,
+  } as any,
+  publicAPI: {
+    focusItem: () => {},
+    getItem: () => ({}),
+    setItemExpansion: () => {},
+  },
+  runItemPlugins: () => ({
+    rootRef: null,
+    contentRef: null,
+  }),
+  wrapItem: ({ children }) => children,
+  wrapRoot: ({ children }) => children,
+  disabledItemsFocusable: false,
+  indentationAtItemLevel: false,
+  icons: {
+    slots: {},
+    slotProps: {},
+  },
+  selection: {
+    multiSelect: false,
+    checkboxSelection: features.checkboxSelection ?? false,
+    disableSelection: false,
+  },
+  rootRef: {
+    current: null,
+  },
+});