Some errors when I using registration

[WZFish]

I try the wholebrain using the code and data in wholebrain website for single section:

regi<-registration(FFC_filename, coordinate=0.38, filter=seg$filter)
and return:
Loading image:/home/wzfish/Downloads/stitched_FFC_section001/stitched_FFC_section001.tif
====== LOADING DONE ======
Resizing to: 0.0168% of original size.
Image type: CV_16U_2
Error in get.contour(file, thresh = brain.threshold, resize = resize, :
OpenCV(4.3.0) /tmp/RtmpHbUcHd/opencv-4.3.0/modules/imgproc/src/thresh.cpp:1529: error: (-215:Assertion failed) src.type() == CV_8UC1 in function 'threshold'

I installed the opencv4.3.0 by RopenCVLite.
Thank you!

[tractatus]

Hi

Did you run segmentation and selected a threshold for where to find the brain section in your image?

Without it the algorithm will default to Outsu threshold which requires 8-bit image.

You can check if you have it set by:

seg$filter$brain.threshold

All the best,
Daniel