diff --git a/.github/workflows/build_and_test.yml b/.github/workflows/build_and_test.yml
index 22df4232ea294..669cd76bd72fa 100644
--- a/.github/workflows/build_and_test.yml
+++ b/.github/workflows/build_and_test.yml
@@ -366,6 +366,7 @@ jobs:
             pyspark-pandas-connect-part3
     env:
       MODULES_TO_TEST: ${{ matrix.modules }}
+      PYTHON_TO_TEST: 'python3.9'
       HADOOP_PROFILE: ${{ inputs.hadoop }}
       HIVE_PROFILE: hive2.3
       GITHUB_PREV_SHA: ${{ github.event.before }}
@@ -447,7 +448,7 @@ jobs:
           export SKIP_PACKAGING=false
           echo "Python Packaging Tests Enabled!"
         fi
-        ./dev/run-tests --parallelism 1 --modules "$MODULES_TO_TEST"
+        ./dev/run-tests --parallelism 1 --modules "$MODULES_TO_TEST" --python-executables "$PYTHON_TO_TEST"
     - name: Upload coverage to Codecov
       if: fromJSON(inputs.envs).PYSPARK_CODECOV == 'true'
       uses: codecov/codecov-action@v2
diff --git a/.github/workflows/build_python.yml b/.github/workflows/build_python.yml
new file mode 100644
index 0000000000000..94d64a825d876
--- /dev/null
+++ b/.github/workflows/build_python.yml
@@ -0,0 +1,44 @@
+#
+# Licensed to the Apache Software Foundation (ASF) under one
+# or more contributor license agreements.  See the NOTICE file
+# distributed with this work for additional information
+# regarding copyright ownership.  The ASF licenses this file
+# to you under the Apache License, Version 2.0 (the
+# "License"); you may not use this file except in compliance
+# with the License.  You may obtain a copy of the License at
+#
+#   http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing,
+# software distributed under the License is distributed on an
+# "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY
+# KIND, either express or implied.  See the License for the
+# specific language governing permissions and limitations
+# under the License.
+#
+
+name: "Python - PyPy3.8 (master)"
+
+on:
+  schedule:
+    - cron: '0 15 * * *'
+
+jobs:
+  run-build:
+    permissions:
+      packages: write
+    name: Run
+    uses: ./.github/workflows/build_and_test.yml
+    if: github.repository == 'apache/spark'
+    with:
+      java: 17
+      branch: master
+      hadoop: hadoop3
+      envs: >-
+        {
+          "PYTHON_TO_TEST": "pypy3"
+        }
+      jobs: >-
+        {
+          "pyspark": "true"
+        }
diff --git a/dev/run-tests.py b/dev/run-tests.py
index cf0db66fba1cc..3fe79af58d717 100755
--- a/dev/run-tests.py
+++ b/dev/run-tests.py
@@ -375,7 +375,7 @@ def run_scala_tests(build_tool, extra_profiles, test_modules, excluded_tags, inc
         run_scala_tests_sbt(test_modules, test_profiles)
 
 
-def run_python_tests(test_modules, parallelism, with_coverage=False):
+def run_python_tests(test_modules, test_pythons, parallelism, with_coverage=False):
     set_title_and_block("Running PySpark tests", "BLOCK_PYSPARK_UNIT_TESTS")
 
     if with_coverage:
@@ -390,6 +390,7 @@ def run_python_tests(test_modules, parallelism, with_coverage=False):
     if test_modules != [modules.root]:
         command.append("--modules=%s" % ",".join(m.name for m in test_modules))
     command.append("--parallelism=%i" % parallelism)
+    command.append("--python-executables=%s" % test_pythons)
     run_cmd(command)
 
 
@@ -423,6 +424,12 @@ def parse_opts():
         default=8,
         help="The number of suites to test in parallel (default %(default)d)",
     )
+    parser.add_argument(
+        "--python-executables",
+        type=str,
+        default="python3.9",
+        help="A comma-separated list of Python executables to test against (default: %(default)s)",
+    )
     parser.add_argument(
         "-m",
         "--modules",
@@ -651,6 +658,7 @@ def main():
     if modules_with_python_tests and not os.environ.get("SKIP_PYTHON"):
         run_python_tests(
             modules_with_python_tests,
+            opts.python_executables,
             opts.parallelism,
             with_coverage=os.environ.get("PYSPARK_CODECOV", "false") == "true",
         )