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SeqDBI.pm
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SeqDBI.pm
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#!/tools/bin/perl -w
##---------------------------------------------------------------------------##
## File:
## @(#) SeqDBI.pm
## Author:
## Robert Hubley <rhubley@systemsbiology.org>
## Description:
## A simple sequence database interface. Support for
## adding/removing and editing of sequences and simple
## description type annotations.
##
#******************************************************************************
#* Copyright (C) Institute for Systems Biology 2003-2004 Developed by
#* Arian Smit and Robert Hubley.
#*
#* This work is licensed under the Open Source License v2.1. To view a copy
#* of this license, visit http://www.opensource.org/licenses/osl-2.1.php or
#* see the license.txt file contained in this distribution.
#*
###############################################################################
# ChangeLog
#
# $Log$
#
################################################################################
#
## To Do:
#
# Provide a mechanism for providing alphabet distributions
# This will help with determining if all characters are
# masked. Also helps with GC content etc.
#
=head1 NAME
SeqDBI
=head1 SYNOPSIS
use SeqDBI;
Usage:
$mySeqDBI = $SeqDBI->new();
=head1 DESCRIPTION
A simple sequence database interface. Support for
adding/removing and editing of sequences and simple description
type annotations.
=head1 SEE ALSO
=over 4
FastaDB, RepeatMasker
=back
=head1 COPYRIGHT
Copyright 2004 Robert Hubley Institute for Systems Biology
=head1 AUTHOR
Robert Hubley <rhubley@systemsbiology.org>
=cut
#
# Module Dependence and Package Definition
#
package SeqDBI;
use strict;
use Carp;
use vars qw(@ISA @EXPORT @EXPORT_OK %EXPORT_TAGS $VERSION);
#
# Constants
#
use constant ReadOnly => 1;
use constant ReadWrite => 2;
require Exporter;
@ISA = qw(Exporter);
@EXPORT = qw();
@EXPORT_OK = qw(min max);
%EXPORT_TAGS = ( all => [ @EXPORT_OK ] );
#
# Version
#
my $VERSION = 0.1;
##-------------------------------------------------------------------------##
## Constructors
##-------------------------------------------------------------------------##
##-------------------------------------------------------------------------##
=head2 new()
Use: my $seqDB = SeqDBI->new();
Must be implemented by concrete classes.
=cut
##-------------------------------------------------------------------------##
sub new {
my $class = shift;
croak "Usage: \$seqDB = SeqDBI->new(); " if ( @_ != 0 );
# Create ourself as a hash
my $this = {};
# Bless this hash in the name of the father, the son...
bless $this, $class;
return $this;
}
##-------------------------------------------------------------------------##
## Public Methods
##-------------------------------------------------------------------------##
##-------------------------------------------------------------------------##
=head2 addSequence()
Use: my $seqID = addSequence( $obj, $name, $description, $sequence );
Add a sequence to the database.
=cut
##-------------------------------------------------------------------------##
sub addSequence {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 removeSequence()
Use: my removeSequence( $obj, $seqID );
Remove a sequence from the database.
=cut
##-------------------------------------------------------------------------##
sub removeSequence {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getSeqCount()
Use: my $seqCount = &getSeqCount( $obj );
The number of sequences contained in this sequence database.
=cut
##-------------------------------------------------------------------------##
sub getSeqCount {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getSeqLength()
Use: my $length = &getSeqLength( $obj, [$seqID] );
The sequence length of the entire database or of a
particular entry.
=cut
##-------------------------------------------------------------------------##
sub getSeqLength {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getSeqLengths()
Use: my @sequenceLength = &getSeqLengths( $obj );
Get an array of sequence length values for all entries
in the database.
=cut
##-------------------------------------------------------------------------##
sub getSeqLengths {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getXNLength()
Use: my $xnLen = &getXNLength( $obj, [$seqID] );
Get the length of $seqID sequence minus the masking and
scaffold characters 'X' and 'N' respectively. If $seqID is
omited the value returned is the length of database
minus all "X" and "N" characters.
=cut
##-------------------------------------------------------------------------##
sub getXNLength {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getXNLengths()
Use: my @xnLengths = &getXNLengths( $obj );
Get the length of each sequence minus 'X' and 'N' characters
( case insensitive ). The values are returned in an array.
=cut
##-------------------------------------------------------------------------##
sub getXNLengths {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getGCLength()
Use: my $gcLen = &getGCLength( $obj, [$seqID] );
Get the length of $seqID sequence minus all characters
except "G" and "C" ( case insensitive ). If $seqID is
omited the value returned is the length of database
minus all characters except "G" and "C".
=cut
##-------------------------------------------------------------------------##
sub getGCLength {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getGCLengths()
Use: my @gcLengths = &getGCLengths( $obj );
Get the length of each sequence minus all characters
except "G" and "C" ( case insensitive ). The values
are returned in an array.
=cut
##-------------------------------------------------------------------------##
sub getGCLengths {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getSubtLength()
Use my $subtLen = &getSubtLength( $obj, [$seqID] );
Get length of sequence minus the ambiguous characters.
=cut
##-------------------------------------------------------------------------##
sub getSubtLength {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getIDS()
Use my @seqIDs = &getIDS( $obj );
Get an array of all sequence identifiers in the database.
=cut
##-------------------------------------------------------------------------##
sub getIDs {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getDescriptors()
Use my @descriptors = &getDescriptors( $obj );
Get an array of all sequence descriptions contained in the database.
=cut
##-------------------------------------------------------------------------##
sub getDescriptors {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getDescription()
Use: my $desc = &getDescription( $obj, $seqID);
Get the sequence description text for a given $seqID.
=cut
##-------------------------------------------------------------------------##
sub getDescription {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 compact()
Use: my &compact();
Compact database. Provide a means to remove "holes" or space inefficiencies
in the database.
=cut
##-------------------------------------------------------------------------##
sub compact {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getSequence()
Use: my $seqString = &getSequence( $seqID );
Get the sequence string for a given seqID.
=cut
##-------------------------------------------------------------------------##
sub getSequence {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 getSubstr()
Use: my $sequence = &getSubstr( $obj, $seqID, $offset, [$length] );
Get a portion of a sequence from the database. If the $length parameter
is left the return value will be everything following the $offset.
=cut
##-------------------------------------------------------------------------##
sub getSubstr {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
##-------------------------------------------------------------------------##
=head2 setSubstr()
Use: my &setSubstr( $obj, $seqID, $offset, $length, $newSeq);
Replace a portion of a sequence with a new sequence. If the
$newSeq is smaller or larger than the specified length the
database's sequence will be contracted or expanded accordingly
( as the perl substr function ).
=cut
##-------------------------------------------------------------------------##
sub setSubstr {
die "Interface SeqDBI does not implement this function\n"
. "Try instantiating an implementation of this interface\n"
. "instead!\n";
}
1;