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upendrak committed May 2, 2017
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# EVOLINC-I 1.0
# EVOLINC-I 1.3
Evolinc-I is a long intergenic noncoding RNA (lincRNA) identification workflow that also facilitates genome browser visualization of identified lincRNAs and downstream differential gene expression analysis.

Evolinc-I minimally requires the following input data
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```
# Pull the image from CyVerse Dockerhub
docker pull evolinc/evolinc-i:1.1
docker pull evolinc/evolinc-i:1.3
# See the command line help for the image
docker run evolinc/evolinc-i:1.1 -h
docker run evolinc/evolinc-i:1.3 -h
# Run Evolinc-I on the test data. The sample data can be found in the sample_data folder in this repo
git clone https://github.com/Evolinc/Evolinc-I.git
cd Evolinc-I
docker run --rm -v $(pwd):/working-dir -w /working-dir evolinc/evolinc-i:1.1 -c Sample_cuffcompare_out.gtf -g TAIR10_chr1.fasta -r TAIR10_chr1_genes.gff -o test_out -n 4
docker run --rm -v $(pwd):/working-dir -w /working-dir evolinc/evolinc-i:1.3 -c Sample_cuffcompare_out.gtf -g TAIR10_chr1.fasta -r TAIR10_chr1_genes.gff -o test_out -n 4
```

### Using CyVerse Discovery Environment
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#### Step-by-step walkthroughs

Step-by-step walkthrough for running Evolinc-I on DE is available [here](https://drive.google.com/open?id=0B-ferWixi_V3cmh0QzhJeXRXSE0).
Step-by-step walkthrough for the command-line, with directions on how to change parameters is coming soon.
Information on how to easily create a Cuffmerge/Cuffcompare input file from 1-many SRA IDs within the DE can be found [here](https://drive.google.com/open?id=0B-ferWixi_V3NjVpdENLUXhLZjQ)
Step-by-step walkthrough for the command-line, with directions on how to change parameters is coming soon. Information on how to easily create a Cuffmerge/Cuffcompare input file from 1-many SRA IDs within the DE can be found [here](https://drive.google.com/open?id=0B-ferWixi_V3NjVpdENLUXhLZjQ)


# Issues
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The sources in this [Github](https://github.com/Evolinc/Evolinc-I) repository, are copyright free. Thus you are allowed to use these sources in which ever way you like. Here is the full [MIT](https://choosealicense.com/licenses/mit/#) license.

# Citing Evolinc-I
Evolinc-I manuscript is currently under review but is available as a [bioRxiv](http://biorxiv.org/content/early/2017/02/20/110148) preprint.
If you have used Evolinc-I manuscript in your research, please cite as below..

*Andrew D. Nelson*, Upendra K. Devisetty*, Kyle Palos, Asher K. Haug-Baltzell, Eric Lyons, Mark A. Beilstein (2017). "Evolinc: a comparative transcriptomics and genomics pipeline for quickly identifying sequence conserved lincRNAs forfunctional analysis". Frontiers in Genetics. 1(10)*

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