This repository covers the scRNA-seq analyses done for ovarian tumor microenvironment research. Single-cell RNA sequencing was performed using primary ovarian cancer (OC) cells and primary cancer-associated fibroblasts (CAFs). The bioinformatics analysis uncovered ligand-receptor interactions between OC cells and CAFs.
Workflow:
- Human Genome Alignment
- Dataset Integration
- Imputation
- Quality Control
- Data Normalizaiton
- Dimensional Reduction
- Differential Expresison Testing
- Cell Type Assignment
- Ligand-Receptor Interaction Analysis