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fix: Handle removal of discordant expected replicates (issue #210) #319

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merged 3 commits into from
Sep 6, 2024

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@jaamarks jaamarks commented Sep 6, 2024

This PR addresses two issues:

  1. Fixes handling of discordant expected replicates (issue discordant expected duplicate removal #210):
  • Previously, the machinery for removing discordant expected replicates was implemented but not functional due to a column name mismatch.
  • This PR updates the _add_analytic_exclusion function in sample_qc_table.py to correctly identify discordant replicates using the actual column name "is_discordant_replicate" from the concordance summary.csv.
  • With this fix, when the user includes the remove_rep_discordant=True flag in config.yml, discordant replicates will be excluded from subject analysis as intended.
  1. Updates bioconductor-illuminaio package:
  • This PR upgrades the bioconductor-illuminaio package to the latest version (v0.44.0) to address dependency issues encountered with the previous version.

fixes #210

…ibgfortran issue

- Updated bioconductor-illuminaio package in illuminaio.yml to version 0.44.0.
- This fixes the previously encountered missing `libgfortran.so.3` dependency.
- The newer illuminaio version avoids installing a conflicting version of
  libgfortran, ensuring proper functionality of R and Rscript needed for the
  `grouped_median_idat_intensity` rule.
@jaamarks jaamarks force-pushed the issue-210-revisit-disc-exp-reps branch 2 times, most recently from 673254b to 4a0a4ec Compare September 6, 2024 19:09
- In test_sample_qc_table.py and _add_analytic_exclusion function.
- This aligns with the data that the function _add_subject_representative
  receives from the concordance summary.
- This ensures consistency with the input data. The column will later
  be renamed to "Expected Replicate Discordance" in subsequent processing.
@jaamarks jaamarks force-pushed the issue-210-revisit-disc-exp-reps branch from 4a0a4ec to 3eff185 Compare September 6, 2024 19:13
@jaamarks jaamarks merged commit 0987eb1 into default Sep 6, 2024
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@jaamarks jaamarks deleted the issue-210-revisit-disc-exp-reps branch September 6, 2024 21:19
@jaamarks jaamarks changed the title fix: Handle removal of discordant expected duplicates (issue #210) bugfix: Handle removal of discordant expected duplicates (issue #210) Oct 2, 2024
@jaamarks jaamarks changed the title bugfix: Handle removal of discordant expected duplicates (issue #210) bugfix: Handle removal of discordant expected replicates (issue #210) Oct 3, 2024
@jaamarks jaamarks changed the title bugfix: Handle removal of discordant expected replicates (issue #210) fix: Handle removal of discordant expected replicates (issue #210) Oct 11, 2024
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discordant expected duplicate removal
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