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Merge pull request #142 from NuttyLogic/dev
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MethylDackel support, Output to stdout option
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NuttyLogic authored Mar 27, 2023
2 parents 7d44bea + 388f4cf commit 85f4225
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3 changes: 3 additions & 0 deletions README.md
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Expand Up @@ -22,6 +22,9 @@ doi:10.1101/2020.10.06.328559](https://academic.oup.com/gigascience/article/10/5
Documentation can be found at [https://bsbolt.readthedocs.io](https://bsbolt.readthedocs.io/en/latest/).

## Release Notes
- v1.6.0
- MethyDackel compatibility
- Option to output alignment to stdout
- v1.5.0
- Improved thread handling for methylation / variant calling.
- Experimental bisulfite aware SNP caller.
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1 change: 1 addition & 0 deletions docs/align.md
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Expand Up @@ -33,6 +33,7 @@ Input / Output Options:
-F1 File path to fastq 1
-F2 File path to fastq 2 [null]
-O File output Prefix
-OS output to stdout [False]
-OT Int number of bam output threads [1]
-DB File path to bsbolt database
-R Str read group header line such as '@RG ID:foo SM:bar' [null]
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3 changes: 2 additions & 1 deletion docs/alignment_output.md
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Expand Up @@ -16,7 +16,8 @@ will always be assigned a mapping quality of zero.
|XB |Z (string)| Read bisulfite conversion position and context | 5zz1x3z11zZ15z8zZ6z2z11z3xz3z13Xz2|
|XC |i (int)| Bisulfite conversion status, 1 if not fully converted, 0 if converted | XC:i:0|
|YC |i (integer)| Bisulfite ambiguous, 1 if ambiguous, not reported if non-ambiguous| YC:i:1 |
|YS |Z (string)| Mapping strand (C=Crick, W=Watson) and alignment conversion pattern (C2T or G2A) | YS:Z:W_C2T (ie. Watson_Cytosine.to.Thymine)|
|YS |Z (string)| Mapping strand (C=Crick, W=Watson) and alignment conversion pattern (C2T or G2A) | YS:Z:W_C2T (ie. Watson_Cytosine.to.Thymine)|
|XG | Z (string) | Mapping strand for MethylDackel compatibility (Crick=GA, Watson=CT)| XG:Z:CT |

### **MD and XB Tags**

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6 changes: 6 additions & 0 deletions docs/index.md
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Expand Up @@ -18,6 +18,12 @@ BSBolt is released under the MIT license.
doi:10.1101/2020.10.06.328559](https://academic.oup.com/gigascience/article/10/5/giab033/6272610)

## Release Notes
- v1.6.0
- MethyDackel compatibility
- Option to output alignment to stdout
- v1.5.0
- Improved thread handling for methylation / variant calling.
- Experimental bisulfite aware SNP caller.
- v1.4.8
- Fixed bug ending alignment when the reference template end greater than reference boundary.
- v1.4.7
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15 changes: 15 additions & 0 deletions docs/variant_api.md
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::: bsbolt.Variant.CallVariants
:docstring:
:members:

::: bsbolt.Variant.BayesVariant
:docstring:
:members:

::: bsbolt.Variant.ProcessVariantRegions
:docstring:
:members:

::: bsbolt.Variant.VariantCall
:docstring:
:members:
1 change: 1 addition & 0 deletions mkdocs.yml
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Expand Up @@ -29,6 +29,7 @@ nav:
- Imputation: imputation_api.md
- Simulation: simulation_api.md
- Utilities: utils_api.md
- Variant Calling: variant_api.md
#- Tutorial: tutorial.md
#- Pipelines: pipelines.md

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