sgRNA-lib-QC

QC CRISPR Pooled Libraries in Addgene

CRISPR Pooled Libraries on Addgene

• website: https://www.addgene.org/crispr/libraries/

QC libraries

• library type: activation, knockout and inhibition
• species: human and mouse
• only genome-wide libraries showed here

1. activation + human (5 libraries)

Name	ID	Library Type	Species	PI	Lentiviral Generation	gRNAs per gene	Total gRNAs
Broad GPP activation Calabrese p65-HSF	92379 (Set A), 92380 (Set B)	Activation	Human	Doench and Root	3rd	3–6	56,762 (Set A), 56,476 (Set B)
CRISPRa-v2	83978, 1000000091	Activation	Human	Weissman	3rd	5, 10	104,540/209,080
Human CRISPR lncRNA Activation Pooled Library	1000000106	Activation	Human	Zhang	3rd	10	96,458
SAM v1 - 3 plasmid system	1000000057 (Zeocin) / 1000000074 (Puromycin)	Activation	Human	Zhang	3rd	3	70,290
SAM v2 - 2 plasmid system	1000000078 (Blasticidin)	Activation	Human	Zhang	3rd	3	70,290

2. activate + mouse (3 libraries)

Name	ID	Library Type	Species	PI	Lentiviral Generation	gRNAs per gene	Total gRNAs
Broad GPP activation Calabrese p65-HSF	92383 (Set A), 92384 (Set B)	Activation	Mouse	Doench and Root	3rd	3–6	67,187 (Set A) / 66,889 (Set B)
CRISPRa-v2	83996, 1000000093	Activation	Mouse	Weissman	3rd	5, 10	107,105 / 214,210
SAM v1 - 3 plasmid system	1000000075 (Puromycin)	Activation	Mouse	Zhang	3rd	3	69,716

3. knockout + human (9 libraries)

Name	ID	Library Type	Species	PI	Lentiviral Generation	gRNAs per gene	Total gRNAs
Activity-optimized genome-wide library	1000000100 Knockout Human Sabatini and Lander 3rd 10 187,535
Broad GPP genome-wide Brunello	73179 (1 plasmid) / 73178 (2 plasmid)	Knockout	Human	Doench and Root	3rd	4	76,441
Human GeCKO v2	1000000048 (1 plasmid), 1000000049 (2 plasmid)	Knockout	Human	Zhang	3rd	6	123,411
Human genome-wide library v1	69763	Knockout	Human	Wu	3rd	4	77,406
Human improved genome-wide library v1	67989 Knockout Human Yusa	3rd	5	90,709
Mini-human AsCpf1-based Human Genome-wide Knockout Library	130630	Knockout	Human	Draetta	3rd	3-4	17,032 arrays
Toronto KnockOut - Version 1	1000000069	Knockout	Human	Moffat	3rd	12	176,500
Toronto KnockOut - Version 3	90294 (1 plasmid), 125517 (2 plasmid)	Knockout	Human	Moffat	3rd	4	70,948
Two plasmid human activity-optimized genome-wide library	1000000095	Knockout	Human	Sabatini and Lander	3rd	10	187,536

4. knockout + mouse (5 libraries)

Name	ID	Library Type	Species	PI	Lentiviral Generation	gRNAs per gene	Total gRNAs
Broad GPP genome-wide Brie	73632 (1 plasmid) / 73633 (2 plasmid)	Knockout	Mouse	Doench and Root	3rd	4	78,637
Mouse GeCKO v2	1000000052 (1 plasmid) / 1000000053 (2 plasmid)	Knockout	Mouse	Zhang	3rd	6	130,209
Mouse improved genome-wide library v2	67988	Knockout	Mouse	Yusa	3rd	5	90,230
Retroviral Mouse Genome-wide CRISPR Knockout Library	104861	Knockout	Mouse	Teichmann	N/A (retroviral)	5	90,230
Two plasmid mouse activity-optimized genome-wide library	1000000096	Knockout	Mouse	Sabatini and Lander	3rd	10	188,509

5. inhibition + human (2 libraries)

Name	ID	Library Type	Species	PI	Lentiviral Generation	gRNAs per gene	Total gRNAs
Broad GPP inhibition Dolcetto	92385 (Set A) / 92386 (Set B)	Inhibition	Human	Doench and Root	3rd	3–6	57,050 (Set A) / 57,011 (Set B)
CRISPRi-v2	83969, 1000000090	Inhibition	Human	Weissman	3rd	5, 10	104,535 / 209,070

6. inhibition + mouse (2 libraries)

Name	ID	Library Type	Species	PI	Lentiviral Generation	gRNAs per gene	Total gRNAs
Broad GPP inhibition Dolomiti	104090 (Set A) / 104091 (Set B)	Inhibition	Mouse	Doench and Root	3rd	3, 6	67,366 (Set A) / 67,194 (Set B)
CRISPRi-v2	83987, 1000000092	Inhibition	Mouse	Weissman	3rd	5, 10	107,415 /214,830