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README update
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shifu chen authored and shifu chen committed Nov 1, 2018
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Expand Up @@ -163,7 +163,7 @@ For example, the last cycle of Illumina sequencing is uaually with low quality,

* For read1 or SE data, the front/tail trimming settings are given with `-f, --trim_front1` and `-t, --trim_tail1`.
* For read2 of PE data, the front/tail trimming settings are given with `-F, --trim_front2` and `-T, --trim_tail2`. But if these options are not specified, they will be as same as read1 options, which means `trim_front2 = trim_front1` and `trim_tail2 = trim_tail1`.
* If you want to limit the read length, you can specify `-b, --max_len1` for read1, and `-B, --max_len2` for read2. If `--max_len1` is specified but `--max_len2` is not, `--max_len2` will be same as `--max_len1`. For example, if '--max_len1' is specified and read1 is longer than `--max_len1`, `fastp` will trim read1 at its tail to make it as long as `max_len1`.
* If you want to limit the read length, you can specify `-b, --max_len1` for read1, and `-B, --max_len2` for read2. If `--max_len1` is specified but `--max_len2` is not, `--max_len2` will be same as `--max_len1`. For example, if `--max_len1` is specified and read1 is longer than `--max_len1`, `fastp` will trim read1 at its tail to make it as long as `--max_len1`.

Please note that the trimming for `--max_len` limitation will be applied at the last step. Following are fastp's processing steps that may orderly affect the read lengthes:
```
Expand All @@ -175,7 +175,7 @@ Please note that the trimming for `--max_len` limitation will be applied at the
6, trim polyG (--trim_poly_g, enabled by default for NovaSeq/NextSeq data)
7, trim polyX (--trim_poly_x)
8, trim adapter by overlap analysis (enabled by default for PE data)
9, trim adapter by adapter sequence (--adapter_sequence, --adapter_sequence_r2)
9, trim adapter by adapter sequence (--adapter_sequence, --adapter_sequence_r2. For PE data, this step is skipped if last step succeeded)
10, trim to max length (---max_len)
```

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