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Update 1_Annotation pre-processing.R
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PoolLab authored Mar 21, 2023
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2 changes: 1 addition & 1 deletion 1_Annotation pre-processing.R
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Expand Up @@ -752,5 +752,5 @@ file.remove("./results.txt")
# b) continuous read mapping from known gene end (delayed cutoff of sequencing reads posterior from known gene end)
# c) examine external evidence (e.g. Allen in situ brain atlas, Human Protein Atlas etc.)

# Based on the latter, provide new gene boundaries under two new columns: "update_start" and "update_end" depending on whether gene is on "-" or "+" strands, respectively. Use excel formulas, if necessary, to scale curation, and save file as "gene_extension_candidates.xlsx".
# Based on the latter, provide new gene boundaries under two new columns: "update_start" and "update_end" depending on whether gene is on "-" or "+" strands, respectively. Use excel formulas, if necessary, to scale curation, and save file as "gene_extension_candidates.csv".
# Resulting manually curated "gene_extension_candidates.csv" will be used as input in the Optimized annotation assembly stage.

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