isolateR 1.0.1

Update: 2024-08-11 (isolateR 1.0.1)

• Updated sanger_assembly function (aka "sanger_consensus" prior to v1.0.1) to allow assembly of paired Sanger sequences
  • This function loads in the CSV results table from isoQC and merges related sequences based on user input.
  • Original file names before isoQC step need to have a common prefix and differentiating suffixes. (e.g. SAMPLE_01_F.ab1, SAMPLE_01_R.ab1).
  • After aligning paired sequences, the consensus sequence is extracted and priority is given to the read with higher quality. Phred quality scores are reassigned in the final output table in a basic way by taking the mean of both input sequences.
  • Note: This function is designed to be used after the isoQC step and before the isoTAX step.
• Fixed warning issue during isoTAX step caused by usage of girafe function from ggiraph package.