pbb-wetlab

Collection of wet lab methods used @PuckerLab.

Edwards DNA extraction protocol

https://www.floridamuseum.ufl.edu/wp-content/uploads/sites/95/2014/02/Edwards1991.pdf

Reference: Edwards et al. (1991, NAR 19:1349)

CTAB-based DNA extraction (high throughput)

GABI-Kat protocol

Reference: Kleinboelting et al., 2012

CTAB-based DNA extraction (ONT sequencing)

protocol available at protocols.io

Reference: Siadjeu C., Pucker B., Viehoever P., Albach D. and Weisshaar B. (2020). High contiguity de novo genome sequence assembly of Trifoliate yam (Dioscorea dumetorum) using long read sequencing. Genes. doi: 10.3390/genes11030274.