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2023_Garcia-Fossa_Cruz_CurrentProtocols

Scripts for Current Protocols paper.

Notebooks and dependencies

  • To run the notebooks (support notebooks and Basic_Protocol_2), use Google Colab or create an environment using environment.yml file;

  • Clone this repository and cd to the folder;

  • Open Anaconda prompt and paste the following:

    conda env create -f environment.yml
    
  • Will create an environment with the dependencies specified. To activate:

    conda activate interpret_profiles
    

Retrieve representative single-cells notebook

To visualize random or representative cell in a given group and field of view, make a copy of the notebook in your Google Drive and follow the instructions available in the Basic Protocol 2 [1]:

Open in Colab

Cite

Please cite our paper if using this repository

[1] Garcia-Fossa F, Cruz MC, Haghighi M, de Jesus MB, Singh S, Carpenter AE, Cimini BA. Interpreting Image-based Profiles using Similarity Clustering and Single-Cell Visualization. Curr Protoc. 2023 Mar;3(3):e713. doi: 10.1002/cpz1.713. PMID: 36921124; PMCID: PMC10027367.

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