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src/main/macro/interactive_webcam_acquisition_move_rect.ijm
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// CLIJ example macro: interactive_webcam_acquisition_move_rect.ijm | ||
// | ||
// This macro shows how use a webcam with CLIJ. | ||
// It furthermore demonstrates how to move an ROI according to | ||
// how the center of mass inside that ROI is changing. | ||
// | ||
// To run that script, please activate the clij and clij2 update | ||
// sites in Fiji. | ||
// https://clij.github.io/clij-docs/installationInFiji | ||
// | ||
// Author: Robert Haase | ||
// March 2020 | ||
// This macro shows how use a webcam with CLIJ. | ||
// It furthermore demonstrates how to move an ROI according to | ||
// how the center of mass inside that ROI is changing. | ||
// | ||
// To run that script, please activate the clij and clij2 update | ||
// sites in Fiji. | ||
// https://clij.github.io/clij-docs/installationInFiji | ||
// | ||
// Author: Robert Haase | ||
// March 2020 | ||
// --------------------------------------------- | ||
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run("Close All"); | ||
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// init GPU | ||
run("CLIJ Macro Extensions", "cl_device="); | ||
run("CLIJ2 Macro Extensions", "cl_device="); | ||
Ext.CLIJ2_clear(); | ||
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// list cameras and get the last entry | ||
Ext.CLIJx_listWebcams(); | ||
camera_index = getResult("Camera_Index", nResults() - 1); | ||
image_width = getResult("Image_Width", nResults() - 1); | ||
image_height = getResult("Image_Height", nResults() - 1); | ||
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// start acquisition | ||
Ext.CLIJx_startContinuousWebcamAcquisition(camera_index, image_width, image_height); | ||
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// an empty image serves as cancel button for the acquisition | ||
cancelText = "Close this window to cancel acquisition"; | ||
newImage(cancelText, "8-bit", 400, 10, 1); | ||
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run("Set Measurements...", "center bounding redirect=None decimal=3"); | ||
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time = getTime(); | ||
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old_x = 0; | ||
old_y = 0; | ||
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// define minimu / maximum shift | ||
max_shift = 10; | ||
min_shift = 0.1; | ||
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for (i = 0; i < 10000; i++) { | ||
print("\\Clear"); | ||
print(i); | ||
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// measure frame delay | ||
deltaTime = getTime() - time; | ||
time = getTime(); | ||
print("frame delay: " + deltaTime); | ||
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// list cameras and get the last entry | ||
Ext.CLIJx_listWebcams(); | ||
camera_index = getResult("Camera_Index", nResults() - 1); | ||
image_width = getResult("Image_Width", nResults() - 1); | ||
image_height = getResult("Image_Height", nResults() - 1); | ||
// start acquisition | ||
Ext.CLIJx_startContinuousWebcamAcquisition(camera_index, image_width, image_height); | ||
// an empty image serves as cancel button for the acquisition | ||
cancelText = "Close this window to cancel acquisition"; | ||
newImage(cancelText, "8-bit", 400, 10, 1); | ||
run("Set Measurements...", "center bounding redirect=None decimal=3"); | ||
time = getTime(); | ||
old_x = 0; | ||
old_y = 0; | ||
// define minimu / maximum shift | ||
max_shift = 10; | ||
min_shift = 0.1; | ||
for (i = 0; i < 10000; i++) { | ||
print("\\Clear"); | ||
print(i); | ||
// measure frame delay | ||
deltaTime = getTime() - time; | ||
time = getTime(); | ||
print("frame delay: " + deltaTime); | ||
// acquire image and send it to GPU | ||
input = "input"; | ||
Ext.CLIJx_captureWebcamImage(input, camera_index, image_width, image_height); | ||
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// flip the input | ||
flipped = "flipped"; | ||
Ext.CLIJ2_flip3D(input, flipped, true, false, false); | ||
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input_r = "input_r"; | ||
Ext.CLIJ2_copySlice(flipped, input_r, 0); | ||
input_g = "input_g"; | ||
Ext.CLIJ2_copySlice(flipped, input_g, 1); | ||
input_b = "input_b"; | ||
Ext.CLIJ2_copySlice(flipped, input_b, 2); | ||
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// view the image | ||
Ext.CLIJx_showRGB(input_r, input_g, input_b, "viewer"); | ||
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// if there is any ROI | ||
run("Measure"); | ||
roi_width = getResult("Width", nResults() - 1); | ||
roi_height = getResult("Height", nResults() - 1); | ||
if (image_width != roi_width || image_height != roi_height) { | ||
Roi.setStrokeWidth(2); | ||
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// determine center of mass | ||
center_of_mass_x = getResult("XM", nResults() - 1); | ||
center_of_mass_y = getResult("YM", nResults() - 1); | ||
print("center of mass x" + center_of_mass_x); | ||
print("center of mass y" + center_of_mass_y); | ||
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abs_delta_x = abs(old_x - center_of_mass_x); | ||
abs_delta_y = abs(old_y - center_of_mass_y); | ||
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// if shift is large enough, reposition the ROI | ||
if (abs_delta_x < max_shift && abs_delta_x > min_shift && abs_delta_y < max_shift && abs_delta_y > min_shift) { | ||
makeRectangle(center_of_mass_x - roi_width / 2, center_of_mass_y - roi_height / 2, roi_width, roi_height); | ||
} | ||
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old_x = center_of_mass_x; | ||
old_y = center_of_mass_y; | ||
} | ||
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// in case the first window was closed, cancel the program | ||
if (!isOpen(cancelText)) { | ||
print("Exiting"); | ||
break; | ||
} | ||
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if (i > 0) { | ||
//break; | ||
} | ||
wait(50); | ||
} | ||
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// stop acquisition | ||
Ext.CLIJx_stopContinuousWebcamAcquisition(camera_index); | ||
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print("Bye"); | ||
input = "input"; | ||
Ext.CLIJx_captureWebcamImage(input, camera_index, image_width, image_height); | ||
// flip the input | ||
flipped = "flipped"; | ||
Ext.CLIJ2_flip3D(input, flipped, true, false, false); | ||
input_r = "input_r"; | ||
Ext.CLIJ2_copySlice(flipped, input_r, 0); | ||
input_g = "input_g"; | ||
Ext.CLIJ2_copySlice(flipped, input_g, 1); | ||
input_b = "input_b"; | ||
Ext.CLIJ2_copySlice(flipped, input_b, 2); | ||
// view the image | ||
Ext.CLIJx_showRGB(input_r, input_g, input_b, "viewer"); | ||
// if there is any ROI | ||
run("Measure"); | ||
roi_width = getResult("Width", nResults() - 1); | ||
roi_height = getResult("Height", nResults() - 1); | ||
if (image_width != roi_width || image_height != roi_height) { | ||
Roi.setStrokeWidth(2); | ||
// determine center of mass | ||
center_of_mass_x = getResult("XM", nResults() - 1); | ||
center_of_mass_y = getResult("YM", nResults() - 1); | ||
print("center of mass x" + center_of_mass_x); | ||
print("center of mass y" + center_of_mass_y); | ||
abs_delta_x = abs(old_x - center_of_mass_x); | ||
abs_delta_y = abs(old_y - center_of_mass_y); | ||
// if shift is large enough, reposition the ROI | ||
if (abs_delta_x < max_shift && abs_delta_x > min_shift && abs_delta_y < max_shift && abs_delta_y > min_shift) { | ||
makeRectangle(center_of_mass_x - roi_width / 2, center_of_mass_y - roi_height / 2, roi_width, roi_height); | ||
} | ||
old_x = center_of_mass_x; | ||
old_y = center_of_mass_y; | ||
} | ||
// in case the first window was closed, cancel the program | ||
if (!isOpen(cancelText)) { | ||
print("Exiting"); | ||
break; | ||
} | ||
if (i > 0) { | ||
//break; | ||
} | ||
wait(50); | ||
} | ||
// stop acquisition | ||
Ext.CLIJx_stopContinuousWebcamAcquisition(camera_index); | ||
print("Bye"); |