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PVCA (Principal Variance Component Analysis) for sequencing read counts

Author: Donghyung Lee

Note: The function is written based on the 'pvcaBatchAssess' function of the PVCA R package and slightly changed to make it more efficient and flexible. (http://watson.nci.nih.gov/bioc_mirror/packages/release/bioc/manuals/pvca/man/pvca.pdf)

Description: Function for Principal Variance Component Analysis

Input:

  1. counts: normalized(e.g. TMM) or log-transformed reads count matrix from sequencing data (row:gene/feature, col:sample) 
           
  2. meta: meta data matrix containing predictor variables (row:sample, col:predictor)
  
  3. threshold: proportion of the variation in read counts explained by k top PCs. This value determines the number of PCs to be used in pvca. 
  
  4. inter: TRUE/FALSE - include/do not include pairwise interactions of predictors

Output:

   a vector of proportions of the variation in read counts data explained by each predictor.

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