Genome Biology release

TraSig (Trajectory-based Signalling genes inference) identifies interacting cell types pairs and significant ligand-receptors based on the expression of genes as well as the pseudo-time ordering of cells. For any two groups of cells that are expected to overlap in time, TraSig takes the pseudo-time ordering for each group and the expression of genes along the trajectory as input and then outputs an interaction score and p-value for each possible ligand-receptor pair. It also outputs a summary score for cell type pairs by combining individual ligand-receptors' scores.