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BioinformaticsUPF

How to start

First we need to download VirtualBox software to run the image of the course.

Donwload VirtualBox

Ubuntu 16.04

First we will add the source list of VB to our host

sudo nano /etc/apt/sources.list

Then we will append the following line

deb http://download.virtualbox.org/virtualbox/debian xenial contrib

Press Ctrl+0 to save and Ctrl+X to close the file

Now, we need the Oracle public key

wget -q https://www.virtualbox.org/download/oracle_vbox_2016.asc -O- | sudo apt-key add -

And finally, we will updater the index and install VB

sudo apt update
sudo apt install virtualbox-5.1

Windows

For Windows platform, we only need to go the the VirtualBox site and download the executable. You can find it here https://www.virtualbox.org/wiki/Downloads

MacOsX

Coming soon...

Download the image

Download the VB image VB

And we will get a file with the extension .ova

Run VB with our image

Now, open VirtualBox and follow the steps to import the image.

Click on File > Import Appliance (Image below)

Image 001

Then click on the icon on the right and select the image you have download in the step before

Image 002

Then, is just the 1st rule of Installation Guide, it means...Import>>Next>>Next>>...>>Accept at the end of the proccess it can take some time to import the image. Keep Calm

Image 003

The last step is to run the image. Select the image and click on Start and it will run and popup a new windows with all the enviroment and software ready to use.

Image 004

Software Included

  • Nextflow (ver. 0.26.4.4741)
  • Python (ver. 2.7.12)
    • BioPython
  • Blast (ver. 2.2.24)
  • SeqAnn Package
  • T_Coffee (dev@20171228_16:49)
  • ViennaRNA Package (ver. 2.4.3)

We need to add some extra software in our image.

Open a text editor and copy this code:

wget http://evolution.gs.washington.edu/phylip/download/phylip-3.697.tar.gz
tar -zxvf phylip-3.697.tar.gz
cd phylip-3.697/src
make -f Makefile.unx install
cd ..
cd exe
export PATH=$PATH:$PWD
cd ../../
rm phylip-3.697.tar.gz

Save the file as installPhy.sh then change the permission of the file with this command chmod +x installPhy.sh and now run the script with ./installPhy.sh. It will install and configure the software PHYLIP

You can find the course material here: http://edgargarriga.com/2018_upf_bioinfo.zip

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