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Software to process single-molecule and diffraction-limited polarisation camera image data.

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DOI

RRID:SCR_025343

POLCAM-SR

Software for processing single-molecule and diffraction limited polarisation camera image data.

The POLCAM-SR software is described in our bioRxiv preprint. Documentation can be found on the POLCAM website.

Requirements

  1. MATLAB license (tested and developed using MATLAB R2023a)
  2. MATLAB Toolboxes: Parallel Computing, Optimization, Image processing, Curve fitting, Statistics and Machine Learning
  3. Mac or Windows

Installation of MATLAB plugin version

You can install POLCAM-SR as a MATLAB plugin. Click here to download the installer.

Installation of full version

  1. Clone the repository
  2. Run the file POLCAM-SR/POLCAM-SR.mlapp

Issues

If you encounter any problems, please file an issue along with a detailed description. Please also do not hesitate to contact us at eb758@cam.ac.uk with any questions about the installation and/or use of the software.

Citing POLCAM-SR

If you use POLCAM-SR in your research, please cite our preprint describing the software.

E. Bruggeman, O. Zhang, L-M. Needham, M. Körbel, S. Daly, M. Cheetham, R. Peters, T. Wu, A.S. Klymchenko, S.J. Davis , E.K. Paluch , D. Klenerman , M.D. Lew , K. O’Holleran and S.F. Lee
POLCAM: Instant molecular orientation microscopy for the life sciences
bioRxiv (2023). https://doi.org/10.1101/2023.02.07.527479