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R script for assessing the DNA probes made in PaintSHOP (for Drosophila melanogaster)

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PaintSHOP DNA QC

R script for assessing the uniqueness of DNA FISH oligo probes' mapping to dm6 genome. Probes are generated in PaintSHOP and loaded to the 'data' folder. Run the script 'probeQC.R' which will produce these plots in 'plots' directory: karyoplot and genomic probe counts

Requirements

  • conda and mamba
  • conda environment 'align' with bowtie2 and samtools installed
    conda config --add channels defaults
    conda config --add channels bioconda
    conda config --add channels conda-forge
    conda config --set channel_priority strict
    mamba create -n align -c bioconda bowtie2 samtools
    

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R script for assessing the DNA probes made in PaintSHOP (for Drosophila melanogaster)

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