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* Add methylation calls to bedmethyl * Output whatshap stats, and add pipeline presets * Fix mosdepth not running for all samples * Fix methylation sample mix * fix dipcall not working * Add fastq and bam stats * fix dipcall not working * Rework modules and subworkflows to match new-found knowledge about single element channels * Added TRGT for pacbio-data * Fix repeat versions * Fix samtools index publishdirs * Add back --secondary=no to minimap2 align * Fix cramino phased and unphased * Update README.md * Fix dipcall sample name in vcf * Fix trgt container adress * try again to fix trgt container adress * remove TRVZ because it will create too many processes * completely remove TRVZ * update modules conf, missing output files, index bams and fix containers * Fix minimap2 index always using default params * minimap index needs to be built separate for dipcall as well * tweak process requirements * Improve whatshap phasing * Add hiphase * add missing bcftools modules * Add samplesheet, extra_gcvf and extra_snfs validation * update hifiasm * completely remove PED and rely on samplesheet * fix methylation bug * update modkit, removes now unneccesary creation of haplotype bams * Formatting, remove unneccessary inputs * fix leftover flag in pileup * Add skip for phasing and remove more PED references * Simplify sniffles, and put back dipcall par * Update README.md * Add skip for QC * Update hiphase output files * Add hificnv to workflow * nf-download not working.. * add config * Add missing file column to snfs schema * Add hificnv to workflow * nf-download not working.. * add config * Update README.md Remove reference to ped-file * Update README.md * Update align.nf If using BAM-files as input for pbmm2, then don't output those. * Replaces pbmm2 with minimap2, using the nf-core modules * Fix typo * Add missing diff..linting fails because of the container URL, not sure how to fix? * linting working for all but samtools/faidx * Update README.md * Added DeepTrio support (incl. GPU for both DV and DT), trios extractedfrom PED-file * Update README.md * Small clean up. * Add deeptrio, GPU support and fastqc (should get better at keeping features separate) * fix minimap2 singularity version * Added dipcall * Add dipcall as process * Might have messed up git stash * Rewrite fix_trio process in Groovy, add hifiasm-trio and dipcall * Merge updated TEMPLATE * Update modules after TEMPLATE upgrade * More modules fixes to bring it closer to nf-core * Prettier * Replace fix_trio process for deeptrio input * remove nf-core images and add pipeline summary * Update README.md * fix color * Allow to skip either assembly or read_map calling * Update docs * Remove references to non-existing slack * Add tandem-regions and reference back to sniffles + skip snv-calling * reorganise publishdirs * move yak-versions into trio workflow * Fix non-trio hifiasm and add more rememberable skips * Fix dipcall module? * Update genome_assembly.nf * See if this way works * See if this way works-2 * See if this way works-3 * nf-core download would not pull docker image when the container is specified inside single quotes * Update dipcall to use PED-file in non-trio mode * Update base config to better represent needs * Change hifiasm to nf-core module, but make it output lowQ beds as well * add mosdepth * fix dipcall bug * Revert "fix dipcall bug" This reverts commit 36b9768. * Revert "Revert "fix dipcall bug"" - sorry git log This reverts commit 713272e. * Hope to have fixed all dipcall bugs - but need more testing * Change local GLNexus to nf-core module * Make tandem_repeats optional, since it might not be that great? * Update README.md * Add methylation calls to bedmethyl * Output whatshap stats, and add pipeline presets * Fix mosdepth not running for all samples * Fix methylation sample mix * fix dipcall not working * Add fastq and bam stats * fix dipcall not working * Rework modules and subworkflows to match new-found knowledge about single element channels * Added TRGT for pacbio-data * Fix repeat versions * Fix samtools index publishdirs * Add back --secondary=no to minimap2 align * Fix cramino phased and unphased * Fix dipcall sample name in vcf * Update README.md * Fix trgt container adress * try again to fix trgt container adress * remove TRVZ because it will create too many processes * completely remove TRVZ * update modules conf, missing output files, index bams and fix containers * Fix minimap2 index always using default params * minimap index needs to be built separate for dipcall as well * tweak process requirements * Improve whatshap phasing * Add hiphase * add missing bcftools modules * Add samplesheet, extra_gcvf and extra_snfs validation * update hifiasm * completely remove PED and rely on samplesheet * fix methylation bug * update modkit, removes now unneccesary creation of haplotype bams * Formatting, remove unneccessary inputs * fix leftover flag in pileup * Add skip for phasing and remove more PED references * Simplify sniffles, and put back dipcall par * Update README.md * Add skip for QC * Update hiphase output files * Add missing file column to snfs schema * Update README.md Remove reference to ped-file * Annotate variants with one database * Change to list of dbs * Fix anno overwriting input with output * Fix anno output file * Add VEP annotation * Update README and schema etc. * Add 'hack' to be able to run ONT-files in TRGT * Update README * Update README.md * Update README.md * Split alignment for faster processing * Call variants per regions in bed/fai * Extracting regions from extra gVCFs takes too long * Singularity image does not contain jemalloc * Grab correct one * glnexus will load full files into db even with bed * split DV input in 13 instead of n regions in BED * forgot wf * Clean up, remove PMD/DT as snv-callers, and ONT_R9 as preset * Remove index split alignments & update README * Update README * Update README.md * Update README.md * Add grouptuple size so downstream wf's don't have to wait for all samples to complete * pipeline would freeze depending on number of regions specified * better outputdirs for aligned reads * Update docs with pipeline parameters * Update README * Update README.md * Remove nf-core reference * Split module configs * Fix most process outdirs * minimap2 soft-clips supplementary reads by default * update hificnv config * Add expected CN-files (for XX/XY), MAF-vcf and option to use an exclude regions file. Also update HiFiCNV version to v.0.1.7 * Use BED file for mosdepth * Fix mosdepth running without BED * Update samtools_cat_sort_index.nf Fix args bug * Fix sniffles args bug * Update short_variant_calling.config Merged SNV-vcfs output files were misssing * Fix actually skipping CNV workflow is specifying so * Update qc_aligned_reads.nf Fix mosdepth bed input * Update qc_aligned_reads.nf * Update split_bed_chunks.py Sometimes, the BED-output was with decimals. Difference between system? * Update split_bed_chunks.py * Update split_bed_chunks.py Check that BED-file does not contain any spaces instead. * Update README.md * adding codeowners * Add test profile and data (#33) Add small test profile and data, hg38 asset files and code formatting. * Update pipeline to run with a small test dataset (#35) Reduce memory requirements, allow gzipped reference, single sample run and add BED file in modkit. * Template merge 2.13.1 (#38) Merge template version 2.13.1 * Fix bcftools merge (#43) Fix bcftools merge --------- Co-authored-by: Anders Jemt <jemten@users.noreply.github.com> * Add Revio BAM test data and a multisample-BAM samplesheet (#50) Add BAM test data and a multisample-BAM samplesheet * Add support for uBAM and multisample test (#51) Add ubam support and multisample test * Update usage docs (#53) update usage docs --------- Co-authored-by: Anders Jemt <jemten@users.noreply.github.com> * use biocontainer for glnexus * change to pacbio registry * run pre-commit * Update CHANGELOG * Fixed mosdepth input channel bug when bedfile is not provided * Update module configs (#65) Update config output directories and add minimal documentation * Fix checking whether input files exist (#67) * Add back files exist check, and fromSamplesheet * Add CNV calling workflow to test profile (#68) * Don't skip cnv calling workflow * Updated CHANGELOG.md * Add SNV annotation test data (#76) * Fix path in snp_dbs.csv (#77) * Refactor parameters validation (#69) * Refactor parateters validation --------- Co-authored-by: Peter Pruisscher <57712924+peterpru@users.noreply.github.com> * Add SNV annotation to test profile (#75) * fix version reporting (#84) * Run a single phasing software instead of three in parallel (#83) * Remove leftover print (#88) * Release prep v.0.1.0 (#89) * bump versions * clear changelog * Name change: Skierfe -> Nallo (#39) Co-authored-by: Anders Jemt <jemten@users.noreply.github.com> * Change testdata location (#91) * move test data * add dummy samplesheet * Update sniffles module (#92) Co-authored-by: Anders Jemt <jemten@users.noreply.github.com> * update deepvariant module (#93) * Add stubs (#94) * update download pipeline (#95) * Update README.md * Update README.md * Update docs/usage.md * Update docs/usage.md * Update docs/usage.md * Update docs/usage.md * Update docs/usage.md * Delete annotations directory (#97) * Delete assets/test_data directory (#99) * Update download_pipeline.yml (#96) * Update snv_annotation.config (#98) Full size test fails because only `BCFTOOLS_NORM_SINGLESAMPLE` and not `BCFTOOLS_NORM` was actually decomposing variants. * Update conf/modules/structural_variant_calling.config Co-authored-by: Anders Jemt <jemten@users.noreply.github.com> * Apply suggestions from code review and full test issues (#107) * Add review suggestions + full test changes * Update CODEOWNERS (#112) * Update CODEOWNERS Change the code-owners to the GitHub team. This way we can more easily change the team and not having to update the CODEOWNERS * Fixed org * Update whatshap stats version to avoid ZeroDivisionError (#133) * Update whatshap stats version to avoid ZeroDivisionError * Update release date * Update bcftools merge (#134) * Bump date in changelog * Since sex is now numberic, update branching criteria * Was fixed before, but was overwritten --------- Co-authored-by: Anders Jemt <jemten@users.noreply.github.com> Co-authored-by: Ram Nanduri <Ram.Nanduri@skane.se> Co-authored-by: Peter Pruisscher <57712924+peterpru@users.noreply.github.com> Co-authored-by: Anders Lind <64251477+pbiology@users.noreply.github.com>
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