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Supporting code for motor neuron maturation manuscript

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Scripts for basic bioinformatics submissions to sge scheduler


Making TrackHubs


python make-trackhub.py examples/trackhub-template.txt trackFolder

usage: make-trackhub.py [-h] [-norm] [-bs BINSIZE] experiment_template trackname

trackFolder can be an existing or new folder in 00_UCSC_tracks

If trackFolder doesn’t exist, it will make one with all the appropriate stuff for a UCSC trackhub

If trackFolder does exist, it will append the lines for the new data to the trackDB.txt

This works for bed, bedpe, and bam.


Alignment


python align.py examples/rna-seq-template.txt START -rsem -rmdup -trim_adaptors

usage: align.py [-h] [-t NTHREADS] [-trim_adaptors] [-rmdup] [-quality QUALITY] [-rsem] [-cufflinks] experiment_template {STAR,bwa,bowtie2}


Peak calling


python call_accessible_regions.py examples/peaks-template.txt

usage: call_accessible_regions.py [-h] [-t NTHREADS] [-p PVAL] [-mapq MAPQ] experiment_template

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Supporting code for motor neuron maturation manuscript

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