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# wdl-cellranger-gex | ||
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WDLized Cell Ranger Gene Expression | ||
WDLized Cell Ranger Gene Expression Pipeline | ||
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## Setup | ||
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```bash | ||
aws s3 cp s3://dp-lab-home/software/install-CellRangerGex-6.0.2.sh - | bash | ||
``` | ||
The pipeline is a part of SCING (Single-Cell pIpeliNe Garden; pronounced as "sing" /siŋ/). For setup, please refer to [this page](https://github.com/hisplan/scing). All the instructions below is given under the assumption that you have already configured SCING in your environment. | ||
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```bash | ||
conda create -n cromwell python=3.7.6 pip | ||
conda activate cromwell | ||
pip install cromwell-tools | ||
``` | ||
## Create Job Files | ||
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Update `secrets.json` with your Cromwell Server address and credentials: | ||
You need two files for processing a V(D)J sample - one inputs file and one labels file. Use the following example files to help you create your configuration file: | ||
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```bash | ||
$ cat secrets.json | ||
{ | ||
"url": "http://ec2-100-26-170-43.compute-1.amazonaws.com", | ||
"username": "****", | ||
"password": "****" | ||
} | ||
``` | ||
- `configs/template.inputs.json` | ||
- `configs/template.labels.json` | ||
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### Reference | ||
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Use one of the URLs below for the reference genome: | ||
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## Running Workflow | ||
Type | `CellRangerGex.reference` | ||
---------- | ----------------------------------------------------------------------------- | ||
GRCh38 | `https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-GRCh38-2020-A.tar.gz` | ||
mm10 | `https://cf.10xgenomics.com/supp/cell-exp/refdata-gex-mm10-2020-A.tar.gz` | ||
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Submit your job: | ||
## Submit Your Job | ||
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```bash | ||
conda activate cromwell | ||
conda activate scing | ||
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./submit.sh \ | ||
-k secrets-aws.json \ | ||
-i configs/sample.inputs.aws.json \ | ||
-l configs/sample.labels.aws.json \ | ||
-k ~/keys/cromwell-secrets.json \ | ||
-i configs/your-sample.inputs.json \ | ||
-l configs/your-sample.labels.json \ | ||
-o CellRangerGex.options.aws.json | ||
``` |
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