Introduction A tool developed for analyzing CG methylation from targeted long-read Tet-assisted pyridine borane sequencing (lrTAPS). Steps 0. summarize the details of all sequencing run run_dataSummarize.sh 1. prepare reference sequence & cg position run_reference.sh 2. map reads to reference with minimap2 run_mapping.sh 3. call methylation from bam file run_callMeth.sh $$methylation = \frac{mC}{mC+uC}$$ $$mC = CA + GT$$ $$uC = CG$$