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MetaSTAARlite_worker_Gene_Centric_Coding_Long_Masks.r
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MetaSTAARlite_worker_Gene_Centric_Coding_Long_Masks.r
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rm(list=ls())
gc()
## load required packages
library(gdsfmt)
library(SeqArray)
library(SeqVarTools)
library(STAAR)
library(STAARpipeline)
library(MetaSTAAR)
library(MetaSTAARlite)
###########################################################
# User Input
###########################################################
## aGDS directory
agds_dir <- get(load("/path_to_the_file/agds_dir.Rdata"))
## Null model
obj_nullmodel <- get(load("/path_to_the_file/obj_nullmodel.Rdata"))
## QC_label
QC_label <- "annotation/filter"
## variant_type
variant_type <- "SNV"
## Annotation_dir
Annotation_dir <- "annotation/info/FunctionalAnnotation"
## Annotation channel
Annotation_name_catalog <- get(load("/path_to_the_file/Annotation_name_catalog.Rdata"))
# Or equivalently
# Annotation_name_catalog <- read.csv("/path_to_the_file/Annotation_name_catalog.csv")
## Use_annotation_weights
Use_annotation_weights <- TRUE
## Annotation name
Annotation_name <- c("CADD","LINSIGHT","FATHMM.XF","aPC.EpigeneticActive","aPC.EpigeneticRepressed","aPC.EpigeneticTranscription",
"aPC.Conservation","aPC.LocalDiversity","aPC.Mappability","aPC.TF","aPC.Protein")
## output path
output_path <- "/path_to_the_output_file/"
## output file name
output_file_name <- "JHS_coding"
## input array id from batch file
arrayid_longmask <- as.numeric(commandArgs(TRUE)[1])
###########################################################
# Main Function
###########################################################
## gene number in job
gene_num_in_array <- 50
group.num.allchr <- ceiling(table(genes_info[,2])/gene_num_in_array)
sum(group.num.allchr)
## analyze large coding masks
arrayid <- c(57,112,112,113,113,113,113,113,113,113)
sub_seq_id <- c(840,543,544,575,576,577,578,579,580,582)
region_spec <- data.frame(arrayid,sub_seq_id)
sub_seq_id <- ((arrayid_longmask-1)*5+1):min(arrayid_longmask*5,length(arrayid))
genes <- genes_info
coding_sumstat <- list()
coding_cov <- list()
for(kk in sub_seq_id)
{
print(kk)
arrayid <- region_spec$arrayid[kk]
sub_id <- region_spec$sub_seq_id[kk]
chr <- which.max(arrayid <= cumsum(group.num.allchr))
## aGDS file
agds.path <- agds_dir[chr]
genofile <- seqOpen(agds.path)
genes_info_chr <- genes_info[genes_info[,2]==chr,]
gene_name <- genes_info_chr[sub_id,1]
results_temp <- coding_MetaSTAARlite_worker(chr=chr,gene_name=gene_name,genofile=genofile,obj_nullmodel=obj_nullmodel,genes=genes,
cov_maf_cutoff=0.05,signif.digits=NULL,
QC_label=QC_label,check_qc_label=TRUE,variant_type=variant_type,
Annotation_dir=Annotation_dir,Annotation_name_catalog=Annotation_name_catalog,
Use_annotation_weights=Use_annotation_weights,Annotation_name=Annotation_name)
coding_sumstat[[gene_name]] <- results_temp$summary_stat_list
coding_cov[[gene_name]] <- results_temp$GTSinvG_rare_list
seqClose(genofile)
}
save(coding_sumstat,file=paste0(output_path,output_file_name,"_sumstat_",arrayid_longmask+379,".Rdata"),compress = "xz")
save(coding_cov,file=paste0(output_path,output_file_name,"_cov_",arrayid_longmask+379,".Rdata"),compress = "xz")