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JoepVanlier committed Sep 17, 2024
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4 changes: 2 additions & 2 deletions docs/theory/force_calibration/surface.rst
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.. _surface_models:

Surface models
--------------

.. _surface_models:

When doing experiments near the surface, the surface has an effect on the friction felt by the bead.
This effect is due to the bead being close to the surface, which changes the drag experienced by the bead.
Shown below is the drag coefficient at frequency zero (constant flow).
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16 changes: 9 additions & 7 deletions docs/tutorial/force_calibration/force_calibration.rst
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Expand Up @@ -31,9 +31,10 @@ The most important parameters are:
Hydrodynamically correct model
""""""""""""""""""""""""""""""

For lateral calibration, it is recommended to use the
For lateral calibration (e.g. `x` and `y`), it is recommended to use the
:ref:`hydrodynamically correct theory<hydro_model_theory>` by setting `hydrodynamically_correct` to
`True`) as it provides an improved model of the underlying physics.
It takes into account inertial and hydrodynamical effects of the fluid and bead(s).
For small beads (< 1 micron) the differences will be small, but for larger beads substantial
differences can occur. There is only one exception to this recommendation, which is when the beads
are so close to the flowcell surface (0.75 x diameter) that this model becomes invalid.
Expand All @@ -45,7 +46,8 @@ Pylake uses values for water and polystyrene for the sample and bead density res
Experiments near the surface
""""""""""""""""""""""""""""

When doing experiments near the surface, it is recommended to provide a `distance_to_surface`.
When doing experiments near the surface, it is recommended to provide a `distance_to_surface` since
the surface has an :ref:`effect on the friction<surface_models>` felt by the bead.
This distance should be the distance from the center of the bead to the surface of the flowcell.
Since it can be challenging to determine this distance, it is recommended to use active calibration
when calibrating near the surface, since active calibration makes calibration far less sensitive
Expand All @@ -60,18 +62,18 @@ to mis-specification of the bead diameter, viscosity and distance to the surface
Technical considerations
************************

Sensor
""""""
Force detector
""""""""""""""

In addition to the model that describes the bead's motion, it is important to take into account the
:ref:`characteristics of the sensor<diode_theory>` used to measure the data.
:ref:`characteristics of the force detector<diode_theory>` used to measure the data.

A silicon diode sensor is characterized by two parameters, a "relaxation factor" `alpha` and
frequency `f_diode`.
These parameters can either be fitted along with the other parameters or characterized independently.

You can check whether a Bluelake calibration item had to estimate parameters from the calibration
data by checking the property `recalibrated.fitted_diode`.
You can check whether Bluelake had to estimate parameters from the calibration
data by checking the property :attr:`~lumicks.pylake.calibration.ForceCalibrationItem.fitted_diode`.
When this property is set to true, it means that the diode parameters were not fixed during the fit.

When fitted, care must be taken that the corner frequency of the power spectrum `fc` is
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