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tombo preprocess annotate_raw_with_fastqs Added sequences to a total of 0 reads. #458

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AlexL-0408 opened this issue Sep 20, 2024 · 0 comments
Open

tombo preprocess annotate_raw_with_fastqs Added sequences to a total of 0 reads. #458

AlexL-0408 opened this issue Sep 20, 2024 · 0 comments

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@AlexL-0408
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Hi, when I was trying to reannotate my fast5 file with my fastq file from Dorado, I ran such command:
tombo preprocess annotate_raw_with_fastqs --fast5-basedir ~/chromatin/fast5_all --fastq-filenames ~/ONT_m6A/chromatin_2.fastq
However, it returned with "Added sequences to a total of 0 reads"
Could you please help me with this problem?
Is there anything to do with how I store my fast5 file?

aa00cee81b5b6b96168b5c2e0d86330
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