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v4.2.0 Evanesco

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@ggabernet ggabernet released this 11 Dec 00:21
d91dd84

Added

  • #334 Added TRUST4 support.
  • #344 Added option to remove V and C primer region when sequence is unknown.
  • #344 Added option to save non-productive sequences
  • #344 Allow for maskprimers align with UMI on both V gene and C gene primers.
  • #344 Update pipeline template to nf-core tools v3.0.2.
  • 357 Deprecate isotype_column parameter in favor of annotating C region from internal c_region alignment or c_primer alignment into the c_call column.

Fixed

  • #344 Avoid saving pRESTO intermediate fastq files in results directory.
  • #344 Simplified pRESTO Maskprimers score and Maskprimers extract processes.
  • #344 Fix clustersets approach, consider both reads clusters annotation.
  • #344 Allow for partial alignments in MakeDB for mouse TCR data, to bypass junction check for TRAV germlines with additional positions (e.g. 84A).
  • #344 Fix parse logs merging dataframes using pandas join to produce logs even when there are failing samples.
  • #344 Use pRESTO Collapseseq with n=0 by default due to runtime issues.

Dependencies

Dependency Old version New version
enchantr 0.1.16 0.1.19
presto 0.7.1 0.7.2

Deprecated parameters

  • isotype_column: if c_call column value is NA, now c_region and c_primer column values respectively are annotated into the c_call column.