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Co-authored-by: Anders Sune Pedersen <37172585+asp8200@users.noreply.github.com>
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maxulysse and asp8200 authored Jul 20, 2022
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## Spark related issues
If you have problems running processes that make use of Spark such as `MarkDuplicates`.
You are probably experiencing issues with the limit of open files in your system.
If you have problems running processes that make use of Spark such, for instance, as `MarkDuplicates`, then that might be due to a limit on the number of simultaneously open files on your system.
You can check your current limit by typing the following:
```bash
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Sarek can process UMI-reads, using [fgbio](http://fulcrumgenomics.github.io/fgbio/tools/latest/) tools.
In order to use reads containing UMI tags as your initial input, you need to include `--umi_read_structure [structure]` in your parameters.
In order to use reads containing UMI tags as your initial input, you need to include `--umi_read_structure <UMI_string>` in your parameters.
This will enable pre-processing of the reads and UMI consensus reads calling, which will then be used to continue the workflow from the mapping steps. For post-UMI processing depending on the experimental setup, duplicate marking and base quality recalibration can be skipped with [`--skip_tools`].
This will enable pre-processing of the reads and UMI consensus reads calling, which will then be used to continue the workflow from the mapping steps. For post-UMI processing depending on the experimental setup, duplicate marking and base quality recalibration can be skipped with `--skip_tools`.
### UMI Read Structure
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