Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Using GATKs mergeVcfs #571

Merged
merged 26 commits into from
Jun 3, 2022
Merged

Using GATKs mergeVcfs #571

merged 26 commits into from
Jun 3, 2022

Conversation

asp8200
Copy link
Contributor

@asp8200 asp8200 commented May 31, 2022

Closes #569

Using GATK4's module mergeVcfs instead of modules/local/concat_vcf/main.nf + bin/concatenateVCFs.sh.

With this PR, the nf-core-module with GATK4's mergeVcfs is now being included in Sarek.

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
    • If you've added a new tool - add to the software_versions process and a regex to scrape_software_versions.py
    • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
    • If necessary, also make a PR on the nf-core/sarek branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@maxulysse
Copy link
Member

<3

@asp8200 asp8200 marked this pull request as ready for review June 2, 2022 12:44
@asp8200 asp8200 requested a review from maxulysse as a code owner June 2, 2022 12:44
@maxulysse
Copy link
Member

some failures in the CI.
I don't care much about gatk4_spark and mutect2 as I know they're difficult, but concerned about haplotypecaller and variantcalling_channel

Copy link
Contributor

@FriederikeHanssen FriederikeHanssen left a comment

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

Great work. Just update the changelog and two indents and then ready to merge 🚀

Edit: Didn't see the failing variantcaller test. Let me see if I can find a reason quickly

subworkflows/nf-core/variantcalling/manta/germline/main.nf Outdated Show resolved Hide resolved
subworkflows/nf-core/variantcalling/manta/somatic/main.nf Outdated Show resolved Hide resolved
@asp8200
Copy link
Contributor Author

asp8200 commented Jun 2, 2022

some failures in the CI. I don't care much about gatk4_spark and mutect2 as I know they're difficult, but concerned about haplotypecaller and variantcalling_channel

@FriederikeHanssen confirmed that there is no problem with the variantcalling_channel. It was apparently just a glitch in the execution of the CI-tests on GitHub. (There seems to be a lot of such glitches with the CI-tests recently.)

The problem with the haplotypecaller-test is that the test expects an output-vcf-file called sample1.vcf.gz but the hyplotypecaller produces a vcf-file called sample1.g.vcf.gz. The problem is also on the dev-branch (6cf0b12). The docs/output.md seem to indicate that the output-vcf-files should be named in another way:

sarek/docs/output.md

Lines 229 to 232 in c60ef1f

**Output directory: `results/VariantCalling/[SAMPLE]/HaplotypeCallerGVCF`**
- `HaplotypeCaller_[SAMPLE].g.vcf.gz` and `HaplotypeCaller_[SAMPLE].g.vcf.gz.tbi`
- `VCF` with Tabix index

CHANGELOG.md Outdated Show resolved Hide resolved
@maxulysse
Copy link
Member

@nf-core-bot fix linting please

@FriederikeHanssen
Copy link
Contributor

Yeah don't worry about haplotypecaller, the PR is already open to fix that subworkflow, just need to finish it. I will fix the CI there

@FriederikeHanssen FriederikeHanssen self-requested a review June 3, 2022 08:19
@FriederikeHanssen FriederikeHanssen merged commit f84f5c6 into nf-core:dev Jun 3, 2022
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

Successfully merging this pull request may close these issues.

3 participants