Support Serology Associated Antigens

Dockerfile

@@ -4,7 +4,7 @@ LABEL MAINTAINER="Pradeep Bashyal"
 
 WORKDIR /app
 
-ARG PY_ARD_VERSION=1.0.11
+ARG PY_ARD_VERSION=1.1.0
 
 COPY requirements.txt /app
 RUN pip install --no-cache-dir --upgrade pip && \

Makefile

@@ -98,10 +98,10 @@ dist: clean ## builds source and wheel package
 	ls -l dist
 
 docker-build: ## build a docker image for the service
-	docker build -t pyard-service:latest .
+	docker build --platform=linux/amd64 -t nmdpbioinformatics/pyard-service:latest .
 
 docker: docker-build ## build a docker image and run the service
-	docker run --rm --name pyard-service -p 8080:8080 pyard-service:latest
+	docker run --platform=linux/amd64 --rm --name pyard-service -p 8080:8080 nmdpbioinformatics/pyard-service:latest
 
 install: clean ## install the package to the active Python's site-packages
 	pip install --upgrade pip

api-spec.yaml

@@ -2,7 +2,7 @@ openapi: 3.0.3
 info:
   title: ARD Reduction
   description: Reduce to ARD Level
-  version: "1.0.11"
+  version: "1.1.0"
 servers:
   - url: 'http://localhost:8080'
 tags:

pyard/__init__.py

@@ -22,12 +22,11 @@
 #    > http://www.opensource.org/licenses/lgpl-license.php
 #
 from .blender import blender as dr_blender
-from .broad_splits import find_splits as find_broad_splits
 from .constants import DEFAULT_CACHE_SIZE
 from .misc import get_imgt_db_versions as db_versions
 
 __author__ = """NMDP Bioinformatics"""
-__version__ = "1.0.11"
+__version__ = "1.1.0"
 
 
 def init(

pyard/ard.py

@@ -113,10 +113,16 @@ def __init__(
         )
 
         # Load Serology mappings
-        broad_splits.broad_splits_ser_mapping = (
-            dr.generate_serology_broad_split_mapping(self.db_connection, imgt_version)
+        broad_splits_mapping, associated_mapping = dr.generate_broad_splits_mapping(
+            self.db_connection, imgt_version
+        )
+        self.serology_mapping = broad_splits.SerologyMapping(
+            broad_splits_mapping, associated_mapping
+        )
+
+        dr.generate_serology_mapping(
+            self.db_connection, self.serology_mapping, imgt_version
         )
-        dr.generate_serology_mapping(self.db_connection, imgt_version)
         # Load V2 to V3 mappings
         dr.generate_v2_to_v3_mapping(self.db_connection, imgt_version)
         # Save IMGT database version
@@ -608,6 +614,12 @@ def is_exp_allele(self, allele):
         """
         return allele in self.allele_group.exp_alleles
 
+    def find_broad_splits(self, allele) -> tuple:
+        return self.serology_mapping.find_splits(allele)
+
+    def find_associated_antigen(self, serology) -> str:
+        return self.serology_mapping.serology_associated_map.get(serology, serology)
+
     def _get_alleles(self, code, locus_antigen) -> Iterable[str]:
         """
         Look up allele code in database and generate alleles

pyard/broad_splits.py

@@ -20,7 +20,8 @@
 #    > http://www.fsf.org/licensing/licenses/lgpl.html
 #    > http://www.opensource.org/licenses/lgpl-license.php
 #
-import re
+
+from pyard.constants import HLA_regex
 
 #
 # Broad, Splits and Associated Antigens
@@ -46,35 +47,35 @@
     "DRB1*06": ["DRB1*13", "DRB1*14"],
 }
 
-# Loaded at runtime
-broad_splits_ser_mapping = None
-
-HLA_regex = re.compile("^HLA-")
-
 
-def find_splits(allele: str) -> tuple:
-    if HLA_regex.search(allele):
-        prefix = True
-        allele_name = allele.split("-")[1]
-    else:
-        prefix = False
-        allele_name = allele
+class SerologyMapping:
+    def __init__(self, broad_splits_mapping, associated_mapping):
+        self.broad_splits_map = broad_splits_mapping
+        self.serology_associated_map = associated_mapping
 
-    if "*" in allele_name:
-        mapping = broad_splits_dna_mapping
-    else:
-        mapping = broad_splits_ser_mapping
+    def find_splits(self, allele: str) -> tuple:
+        if HLA_regex.search(allele):
+            prefix = True
+            allele_name = allele.split("-")[1]
+        else:
+            prefix = False
+            allele_name = allele
 
-    if allele_name in mapping:
-        return _get_mapping(allele_name, mapping, prefix)
+        if "*" in allele_name:
+            mapping = broad_splits_dna_mapping
+        else:
+            mapping = self.broad_splits_map
 
-    for broad in mapping:
-        if allele_name in mapping[broad]:
-            return _get_mapping(broad, mapping, prefix)
+        if allele_name in mapping:
+            return self._get_mapping(allele_name, mapping, prefix)
 
+        for broad in mapping:
+            if allele_name in mapping[broad]:
+                return self._get_mapping(broad, mapping, prefix)
 
-def _get_mapping(broad, mapping, prefix):
-    if prefix:
-        return "HLA-" + broad, list(map(lambda x: "HLA-" + x, mapping[broad]))
-    else:
-        return broad, mapping[broad]
+    @staticmethod
+    def _get_mapping(broad, mapping, prefix):
+        if prefix:
+            return "HLA-" + broad, list(map(lambda x: "HLA-" + x, mapping[broad]))
+        else:
+            return broad, mapping[broad]

pyard/data_repository.py

@@ -26,7 +26,8 @@
 
 import pyard.load
 from pyard.smart_sort import smart_sort_comparator
-from . import db, broad_splits
+from . import db
+from .broad_splits import broad_splits_dna_mapping
 from .load import (
     load_g_group,
     load_p_group,
@@ -216,7 +217,7 @@ def generate_alleles_and_xx_codes_and_who(
     xx_codes = xx_df.groupby(["1d"]).apply(lambda x: list(x["Allele"])).to_dict()
 
     # Update xx codes with broads and splits
-    for broad, splits in broad_splits.broad_splits_dna_mapping.items():
+    for broad, splits in broad_splits_dna_mapping.items():
         for split in splits:
             if broad in xx_codes:
                 xx_codes[broad].extend(xx_codes[split])
@@ -354,7 +355,9 @@ def to_serological_name(locus_name: str):
     return sero_name
 
 
-def generate_serology_mapping(db_connection: sqlite3.Connection, imgt_version):
+def generate_serology_mapping(
+    db_connection: sqlite3.Connection, serology_mapping, imgt_version
+):
     if not db.table_exists(db_connection, "serology_mapping"):
         df_sero = load_serology_mappings(imgt_version)
 
@@ -396,7 +399,7 @@ def generate_serology_mapping(db_connection: sqlite3.Connection, imgt_version):
 
         # map alleles for split serology to their corresponding broad
         # Update xx codes with broads and splits
-        for broad, splits in broad_splits.broad_splits_ser_mapping.items():
+        for broad, splits in serology_mapping.broad_splits_map.items():
             for split in splits:
                 try:
                     sero_mapping[broad] = "/".join(
@@ -450,15 +453,19 @@ def get_db_version(db_connection: sqlite3.Connection):
     return db.get_user_version(db_connection)
 
 
-def generate_serology_broad_split_mapping(
-    db_connection: sqlite3.Connection, imgt_version
-):
+def generate_broad_splits_mapping(db_connection: sqlite3.Connection, imgt_version):
     if not db.table_exists(db_connection, "serology_broad_split_mapping"):
-        sero_mapping = pyard.load.load_serology_broad_split_mapping(imgt_version)
+        sero_mapping, associated_mapping = pyard.load.load_serology_broad_split_mapping(
+            imgt_version
+        )
         db.save_serology_broad_split_mappings(db_connection, sero_mapping)
-        return sero_mapping
+        db.save_serology_associated_mappings(db_connection, associated_mapping)
+        return sero_mapping, associated_mapping
+
+    sero_mapping = db.load_serology_broad_split_mappings(db_connection)
+    associated_mapping = db.load_serology_associated_mappings(db_connection)
 
-    return db.load_serology_broad_split_mappings(db_connection)
+    return sero_mapping, associated_mapping
 
 
 def generate_cwd_mapping(db_connection: sqlite3.Connection):

pyard/db.py

@@ -609,20 +609,36 @@ def load_v2_v3_mappings(db_connection):
 
 def load_serology_broad_split_mappings(db_connection):
     sero_mapping = load_dict(
-        db_connection, "serology_broad_split_mapping", ("serology", "splits")
+        db_connection, "serology_broad_split_mapping", ("broad", "splits")
     )
     sero_splits = {k: v.split("/") for k, v in sero_mapping.items()}
     return sero_splits
 
 
+def load_serology_associated_mappings(db_connection):
+    associated_mapping = load_dict(
+        db_connection, "serology_associated_mappings", ("associated", "antigen")
+    )
+    return associated_mapping
+
+
 def save_serology_broad_split_mappings(db_connection, sero_mapping):
     # Save the `splits` as a "/" delimited string to db
     sero_splits = {sero: "/".join(splits) for sero, splits in sero_mapping.items()}
     save_dict(
         db_connection,
         table_name="serology_broad_split_mapping",
         dictionary=sero_splits,
-        columns=("serology", "splits"),
+        columns=("broad", "splits"),
+    )
+
+
+def save_serology_associated_mappings(db_connection, associated_mapping):
+    save_dict(
+        db_connection,
+        table_name="serology_associated_mappings",
+        dictionary=associated_mapping,
+        columns=("associated", "antigen"),
     )
 
 

pyard/load.py

@@ -20,7 +20,7 @@
 #    > http://www.opensource.org/licenses/lgpl-license.php
 #
 import sys
-from typing import Dict, List
+from typing import Dict, List, Tuple
 from urllib.error import URLError
 
 from pyard.misc import get_G_name, get_2field_allele, get_3field_allele, get_P_name
@@ -38,7 +38,7 @@ def add_locus_name(locus: str, splits: str) -> List:
 # Derived from rel_ser_ser.txt
 # https://raw.githubusercontent.com/ANHIG/IMGTHLA/Latest/wmda/rel_ser_ser.txt
 #
-def load_serology_broad_split_mapping(imgt_version: str) -> Dict:
+def load_serology_broad_split_mapping(imgt_version: str) -> Tuple[Dict, Dict]:
     import pandas as pd
 
     ser_ser_url = f"{IMGT_HLA_URL}{imgt_version}/wmda/rel_ser_ser.txt"
@@ -47,21 +47,36 @@ def load_serology_broad_split_mapping(imgt_version: str) -> Dict:
             ser_ser_url,
             skiprows=6,
             names=["Locus", "A", "Splits", "Associated"],
-            usecols=[0, 1, 2],
             dtype="string",
             sep=";",
-        ).dropna()
+        )
     except URLError as e:
         print(f"Error downloading {ser_ser_url}", e, file=sys.stderr)
         sys.exit(1)
 
-    df_p["Sero"] = df_p["Locus"] + df_p["A"]
-    df_p["Splits"] = df_p[["Locus", "Splits"]].apply(
+    splits_df = df_p[["Locus", "A", "Splits"]].dropna()
+    associated_df = df_p[["Locus", "A", "Associated"]].dropna()
+
+    splits_df["Sero"] = splits_df["Locus"] + splits_df["A"]
+    splits_df["Splits"] = splits_df[["Locus", "Splits"]].apply(
         lambda x: add_locus_name(x["Locus"], x["Splits"]), axis=1
     )
+    splits_df = splits_df.astype({"A": "int32"}).sort_values(by=["Locus", "A"])
+
+    associated_df["Sero"] = associated_df["Locus"] + associated_df["A"]
+    associated_df["Associated"] = associated_df[["Locus", "Associated"]].apply(
+        lambda x: add_locus_name(x["Locus"], x["Associated"]), axis=1
+    )
+    associated_df = associated_df.astype({"A": "int32"}).sort_values(by=["Locus", "A"])
+
+    splits_mapping = splits_df[["Sero", "Splits"]].set_index("Sero")["Splits"].to_dict()
+    associated_mapping = (
+        associated_df.explode("Associated")[["Associated", "Sero"]]
+        .set_index("Associated")["Sero"]
+        .to_dict()
+    )
 
-    sero_mapping = df_p[["Sero", "Splits"]].set_index("Sero")["Splits"].to_dict()
-    return sero_mapping
+    return splits_mapping, associated_mapping
 
 
 def load_g_group(imgt_version):

scripts/pyard

@@ -33,7 +33,7 @@ from pyard.exceptions import InvalidAlleleError, InvalidTypingError, InvalidMACE
 from pyard.misc import get_data_dir, get_imgt_version
 
 
-def find_similar_alleles(prefix):
+def find_similar_alleles(ard, prefix):
     alleles = ard.similar_alleles(prefix)
     if alleles:
         for allele in alleles:
@@ -62,8 +62,8 @@ def expand_mac_code():
     sys.exit(0)
 
 
-def find_broad_splits():
-    mapping = pyard.find_broad_splits(args.splits)
+def find_broad_splits(ard):
+    mapping = ard.find_broad_splits(args.splits)
     if mapping:
         print(f"{mapping[0]} = {'/'.join(mapping[1])}")
     sys.exit(0)
@@ -166,7 +166,7 @@ if __name__ == "__main__":
 
     # Handle --splits option
     if args.splits:
-        find_broad_splits()
+        find_broad_splits(ard)
 
     # Handle --expand-mac option
     if args.expand_mac:
@@ -178,7 +178,7 @@ if __name__ == "__main__":
 
     # Handle --similar option
     if args.similar_allele:
-        find_similar_alleles(args.similar_allele)
+        find_similar_alleles(ard, args.similar_allele)
 
     try:
         if args.cwd:

setup.cfg

@@ -1,5 +1,5 @@
 [bumpversion]
-current_version = 1.0.11
+current_version = 1.1.0
 commit = True
 tag = True
 

setup.py

@@ -36,7 +36,7 @@
 
 setup(
     name="py-ard",
-    version="1.0.11",
+    version="1.1.0",
     description="ARD reduction for HLA with Python",
     long_description=readme,
     long_description_content_type="text/markdown",

tests/features/broad_splits.feature

@@ -31,3 +31,19 @@ Feature: Broad Splits for DNA/Serology
       | DQB1*05  | DQB1*06     | DQB1*01  |
       | B*55     | B*54/B*56   | B*22     |
       | A25      | A26/A34/A66 | A10      |
+
+
+  Scenario Outline: Associated Serology
+
+    Given the serology antigen is <Serology>
+    When looking for associated serology
+    Then the associated serology is found to be <Associated Serology>
+
+    Examples: Alleles to Serology
+      | Serology | Associated Serology |
+      | A23      | A23                 |
+      | A24      | A24                 |
+      | A2403    | A24                 |
+      | DR1403   | DR14                |
+      | DR1404   | DR14                |
+      | B5       | B5                  |