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Minor wording and format changes to README
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lh3 authored Oct 22, 2018
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Expand Up @@ -55,27 +55,28 @@ k-mers by their hashcodes. For data of relatively low coverage, you may
increase this sampling rate by reducing `-S`. This will greatly increase the
peak memory as a cost, though. Option `-e`, which defaults to 3, specifies the
minimum read coverage of an edge in the assembly graph. You may adjust this
option according to the overall sequencing depth, too. For PacBio data,
option according to the overall sequencing depth, too. Option `-A` also helps
relatively low coverage data at the cost of performance. For PacBio data,
`-L5000` often leads to better assemblies emperically, so is recommended.
Please run `wtdbg2 --help` for a complete list of available options or consult
[README-ori.md](README-ori.md) for more help.

The following table shows various command lines and their resource usage for
the assembly step:

|Dataset |Genome|Coverage|Asm options |CPU asm |CPU cns |Real |Peak RAM|
|:-----------------------|-----:|-------:|:------------------|-------:|-------:|------:|-------:|
|[E. coli][pbcr] |4.6Mb |PB x20 |-t32 -L5000 | 39s| 10m34s| 29s| 1.1G|
|[C. elegans][ce] |100Mb |PB x80 |-t32 -L5000 -e4 | 1h00m| 5h06m| 16m16s| 9.5G|
|[Human NA12878][na12878]|3Gb |ONT x36 |-t36 -p19 -AS2 -e2 -L5000|822h28m|115h59m|27h42m|182.1G|
|[Human NA19240][na19240]|3Gb |ONT x35 |-t32 -p19 -AS2 -e2 | 706h30m| 114h45m| 27h33m| 177.5G|
|[C. elegans][ce] |100Mb |PB x80 |-t64 -L5000 | 1h46m| 5h27m| 14m17s| 10.1G|
|[Human CHM1][chm1] |3Gb |PB x60 |-t64 -L10000 | 186h15m| 131h52m| 7h41m| 265.2G|
|[Axolotl][axosra] |32Gb |PB x32 |-t96 -L5000 -AS2 | 3189h| | | 1593.6G|

The first four lines were assembled with 32 threads on a server with Xeon
E5-2683 CPUs at 2GHz. The rest were assembled on a server with Xeon E7-8857
CPUs at 3GHz.
|Dataset |GSize |Cov |Asm options |CPU asm |CPU cns |Real tot| RAM|
|:-----------------------|-----:|-------:|:------------------|-------:|-------:|-------:|-------:|
|[E. coli][pbcr] |4.6Mb |PB x20 |-t32 -L5000 | 39s| 10m34s| 29s| 1.1G|
|[C. elegans][ce] |100Mb |PB x80 |-t32 -L5000 -e4 | 1h00m| 5h06m| 16m16s| 9.5G|
|[Human NA12878][na12878]|3Gb |ONT x36 |-t36 -p19 -AS2 -e2<br/>-L5000|822h28m|115h59m|27h42m|182.1G|
|[Human NA19240][na19240]|3Gb |ONT x35 |-t32 -p19 -AS2 -e2 | 706h30m| 114h45m| 27h33m| 177.5G|
|[C. elegans][ce] |100Mb |PB x80 |-t64 -L5000 | 1h46m| 5h27m| 14m17s| 10.1G|
|[Human CHM1][chm1] |3Gb |PB x60 |-t64 -L10000 | 186h15m| 131h52m| 7h41m| 265.2G|
|[Axolotl][axosra] |32Gb |PB x32 |-t96 -L5000 -AS2 | 3189h| | | 1593.6G|

The timing was obtained on three local servers with different hardware
configurations. There are also run-to-run fluctuations. Exact timing on your
machines may differ.

## Limitations

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