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This repository contains a basic tutorial on performing differential expression analysis using r and the Bioconductor package.

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shehbeel/DE-Analysis-Basic-Tutorial

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DE-Analysis-Basic-Tutorial

This repository contains a very basic tutorial on performing differential expression analysis using the statistical programming language r. This tutorial is meant for anyone who is interested in learning r in order to do basic gene expression analyses using minimal lines of code.

Libraries used:

  • DESeq2
  • ggplot2
  • pheatmap

Dataset used:

  • Subset of GeneLab's GLDS-202 Dataset (Effect of Radiation and Microgravity on Brain Gene Expression [1 month treatment])

Plots made:

  • MA-plot
  • Volcano Plot
  • Heatmap

Sample MA-plot:

GLDS-202_rad_1month_DE_MA-Plot

Sample Heatmap plot:

sig_de_genes_heatmap all_de_genes_heatmap

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This repository contains a basic tutorial on performing differential expression analysis using r and the Bioconductor package.

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