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Draft PR that targets the work done towards TrackMate v9. #263

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Commits on Jul 7, 2024

  1. The Labkit editor can be launched on all time-points at once.

    Shift-click on the button.
    tinevez committed Jul 7, 2024
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  2. Add tooltip for the editor button.

    And remove debug code.
    tinevez committed Jul 7, 2024
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  3. In Labkit editor, labels receive name and color from spots.

    I am not so sure it is a good idea for the color...
    tinevez committed Jul 7, 2024
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  4. Better editor frame title.

    tinevez committed Jul 7, 2024
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  6. Always start the editor in FUSED mode,

    and use the white LUT when the imp is not displayed as a Composite.
    tinevez committed Jul 7, 2024
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  7. Don't crash when editing on images with 1 channel.

    Noticed by @MiniMiette
    tinevez committed Jul 7, 2024
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  9. Store labels on ints, not on shorts.

    Otherwise we get crashes when we have more than 4k labels. Which is not
    what Labkit is optimized for but we will see that in a second time.
    In case we change our minds on the backing integer type, right now
    the labkit launcher and importer classes are generic.
    tinevez committed Jul 7, 2024
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  10. Fix reimporting labels with large IDs in 2D.

    The re-importing of labels from Tabkit to TrackMate could fail for
    2D images and labels with a large index. For instance, it failed
    consistently when trying to re-import labels with an index larger
    than 65643.
    
    This problem roots in the getSpots() method of LabkitImporter. It
    relies on a trick: We get the new label image, and create spots from
    this label image. But we want the new spots to keep track of the index
    in the label image they were generated from.
    
    For this, in 2D, we use the MaskUtils.fromLabelingWithRoi()
    method. These methods accept an image as last argument used to
    read a value in the label image within the spot, that is normally
    used for the quality value of the new spot.
    
    But the SpotRoiUtils.from2DLabelingWithRoi() method converted the
    extra image to ImagePlus (because I was lazy). So the label image
    was effectively cast on ushort for an IntegerType image, hence
    the problem with the max label being 65453.
    
    The solution is to rewrite the from2DLabelingWithRoi() so that
    it does not rely on converting to ImagePlus, but on good old
    iteration with imglib2.
    tinevez committed Jul 7, 2024
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  11. Fix javadoc errors.

    tinevez committed Jul 7, 2024
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Commits on Jul 9, 2024

  1. setFont() utility.

    tinevez committed Jul 9, 2024
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  2. The detection preview is cancelable.

    Provided that the detector that is called is cancelable.
    tinevez committed Jul 9, 2024
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  4. Detection preview panel tweak.

    Make sure the detection preview panel is slanted at the bottom of its display.
    tinevez committed Jul 9, 2024
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Commits on Jul 10, 2024

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  2. Fixed a difficult bug with the LabKit editor reimporter.

    The bug was causing weird issues with unedited spots being deleted,
    unedited spots being duplicated etc.
    
    It took me really long to understand the cause. It was hidden in the
    step where we go from a label image to a collection of spots. Because
    spots are polygons, with simplified contours, there might be some pixels
    on the edges of the object that are not strictly inside the label.
    
    In this importer, we read the label value in one go, by storing it
    in the QUALITY value of the spot, in the MaskUtils class. But since
    the spots have simplified contours, and since the QUALITY value is
    the maximal value iterated over, our approach might fail on border cases:
    - when the contout is approximated and include pixels from another
    object
    - and when this object has a label value higher than the lael of
    the spot.
    
    This commit include a temporary fix: we reiterate over the spot
    but takes the median value iterated over, to make sure we read
    the correct value for the label.
    
    Other attempts will follow, for reference. But a true fix involves
    making a method that returns a map from label value to spot.
    tinevez committed Jul 10, 2024
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  3. Another fix for the LabKit reimporter bug.

    This time we fix it by creating spots that do not have a simplified
    contours. In that case we strictly iterate over the pixels inside
    label and get the correct value.
    However the created spots have a pixelated aspect (contours are
    not simplified), which might not be what the user wants. We should
    let them choose.
    
    Still not the perfect solution, as mentionned in the previous
    commmit.
    tinevez committed Jul 10, 2024
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Commits on Jul 11, 2024

  1. Simplify the LabKit importer.

    Use the new label immg to spot method to get spots AND the
    label they come from.
    tinevez committed Jul 11, 2024
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  2. In LabKit editor, offer to simplify the contour of modified spots.

    Also better message when closing the editor.
    tinevez committed Jul 11, 2024
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Commits on Jul 12, 2024

  1. Let the user edit a ROI in the image with the LabKit editor.

    If a ROI exists in the input image when launching the LabKit editor,
    only the image and the spots present in the ROI are sent to the
    editor. Spots touching the borders are not imported.
    tinevez committed Jul 12, 2024
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Commits on Jul 17, 2024

  1. Fix panning not working when the TrackMate tool is selected.

    Normally pressing space and dragging should move the view, as
    for normal ImageJ tools. The removed line was preventing it.
    tinevez committed Jul 17, 2024
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Commits on Jul 18, 2024

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  2. Fix bug in LabKit editor: avoid spot ID and label conflicts.

    When editing a sub-portion of the image, the spots outside the ROI
    are not imported in the editor. It is possible that the user creates
    a new label in LabKit that will have the same id that an existing
    spot outside the ROI.
    Before this fix, the spots in that case were replaced by the new ones,
    creating a mess.
    The fix consists in reminding what spots are imported in the LabKit
    editor, then compariing to this list the new ids when reimporting
    from the editor.
    tinevez committed Jul 18, 2024
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Commits on Jul 19, 2024

  1. Custom LabKit frame and segmentation component.

    Simplified, removing the menu, the segmentation features and the
    segmenter features.
    Somply done by copy-pasting Matthias classes and removing the lines
    with the features to prune.
    tinevez committed Jul 19, 2024
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  2. Fix another bug with the LabKit importer.

    This time it was due to confusion between a map from spot ID to spots,
    and a map from label value to spot.
    I fixed it by rewriting everything in terms of label values in the
    labeling. So we do not need to keep track of the spot ID anymore. The
    labels could be anything. They are still equal to spot ID + 1, because
    it is convenient to debug.
    tinevez committed Jul 19, 2024
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Commits on Jul 22, 2024

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  4. WIP: Iterative intersection with a plane.

    Still not good enough, the vertices are not iterated in a monotonic
    manner.
    tinevez committed Jul 22, 2024
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  5. Unother attempt, using an edge and a face map.

    Better but still not good enough: some points on the contours
    are repeated.
    tinevez committed Jul 22, 2024
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  6. We actually don't need the vertex list. The edge list is sufficient.

    Also do not add duplicate points on the contour (happens often
    when there is no smoothing).
    Still not good: there are some cases where the loop breaks
    too early.
    tinevez committed Jul 22, 2024
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  8. Rework the demo of mesh intersection.

    Much cleaner. Still does not work for not simple meshes.
    Probably because of border cases where we have vertices that lie
    exactly on the plane we are interesecting with.
    tinevez committed Jul 22, 2024
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  9. A simple data structure to store a 3D mesh in a Spot object.

    This class is the 3D counterpart of SpotRoi. It stores the object
    shape as a mesh, and has (for now) a few methods to facilitate
    painting it and creating it.
    The mesh are stored with coordinates relative to the spot center
    (mesh center is at 0,0,0). The same for the bounding box. The
    mesh coordinates are expected to be in physical coordinates, not
    pixel coordinates.
    tinevez committed Jul 22, 2024
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  11. Utility method for SpotRoi: generate XY coordinates

    and write them in holders provided by the user.
    tinevez committed Jul 22, 2024
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  14. Paint 3D meshes in the HyperStackViewer.

    We simply reslice the mesh at the Z slice currently displayed,
    and paint the intersection as a collection of segments.
    
    I also took the opportunity to refactor a bit the SpotOverlay.
    
    Right now this works but is not optimal:
    1/ There are weird stuff happening at the *top* of the mesh: it's
    like we miss some part of it.
    2/ The slice routine generates a list of disconnected segments. It
    does not show when we paint them, but maybe would be nice to
    reconstruct the collection of contours resulting from the intersection
    of a mesh with a plane.
    3/ We could optimize the slice() routinemaybe  by having an index
    that sorts triangles by their minZ value, and another index that
    sorts them by their maxZ value. This way we could quickly retrieve
    the triangles to sort by two binary-search and one set intersection.
    tinevez committed Jul 22, 2024
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  15. Outdated interactive tests.

    tinevez committed Jul 22, 2024
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  16. Fix javadoc.

    tinevez committed Jul 22, 2024
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  17. Temporary store meshes in absolute physical coordinates.

    While we try to make better slices.
    tinevez committed Jul 22, 2024
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  19. Non working version of a pixel iterator.

    It is working for simple meshes but the ones we have have too
    many border cases and make it fail.
    tinevez committed Jul 22, 2024
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  22. Utility to sort Trove arrays.

    tinevez committed Jul 22, 2024
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  23. Demo simple mesh.

    tinevez committed Jul 22, 2024
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  25. Update the demo with Toby + JY ideas.

    Not good enough yet.
    tinevez committed Jul 22, 2024
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  26. Yeaaahh! Working version of a pixel iterator.

    At least on star-convex objects, but does not assume they are.
    Still very tiny final border cases, where the iteration stops
    early at the poles of an object, but it's nothing unsurmontable.
    Tomorrow.
    tinevez committed Jul 22, 2024
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  29. Update the interactive demo.

    tinevez committed Jul 22, 2024
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  31. Implement quality measurement on the 3D object.

    Without using the mesh actually, we use the bitmask provided.
    tinevez committed Jul 22, 2024
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  33. Iterable for a spot mesh.

    tinevez committed Jul 22, 2024
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  34. Add methods to SpotUtil to return suitable iterables in 2D and 3D.

    With this simple change we get intensity measurements in 3D in a
    mesh for free.
    tinevez committed Jul 22, 2024
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  37. Temporary couple to imagej-mesh-io 0.1.3-SNAPSHOT.

    So that we can have reading from an input stream.
    tinevez committed Jul 22, 2024
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  38. Serialization and deserialization of spot meshes to file.

    The meshes are saved in a ZIP file next to the TrackMate XML file.
    If the TrackMate file is called: trackmate-file.xml, then the file
    that stores the meshes for the model are in a zip file called
    trackmate-file.xml.meshes
    
    This zip file just contains the meshes as PLY files, named with the
    spots ID. If a spot has an ID equal to 1234, then the PLY file that
    stores its mesh is named 1234.ply in the zip file. If the zip file
    does not contain such a file then it means that this spot does not
    have a mesh. If there is not .meshes file, it means that no spot
    have a mesh.
    
    Possible improvement: read all meshes at once, instead of opening and
    closing the zip file for every spot.
    tinevez committed Jul 22, 2024
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  40. Recompute face normals after loading mesh.

    They are not saved not retrieved by the PLY file reader. Should they?
    tinevez committed Jul 22, 2024
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  42. WIP: Rework the Z-slicing of meshes.

    Try to build actual contours across Z sections of the object,
    reusing the ray casting things we have for the iteration.
    Very preliminary and has cases where it does not work.
    Also has a lot of room for optimization and refactoring.
    tinevez committed Jul 22, 2024
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  43. Tweak interactive tests.

    When everything is bugged, they are important.
    tinevez committed Jul 22, 2024
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  47. Rework painting meshes a bit.

    tinevez committed Jul 22, 2024
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  48. Update interactive tests.

    tinevez committed Jul 22, 2024
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  51. Tweak painting of meshes.

    Not important, will go away.
    tinevez committed Jul 22, 2024
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  52. Update interactive tests.

    tinevez committed Jul 22, 2024
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  54. Update interactive tests.

    tinevez committed Jul 22, 2024
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  55. Rework spot mesh iteration.

    Use the contours we got from the ZSlicer. Perform ray cast along
    the X axis. Count how many intersection we crosses to determine
    whether we are inside or outside.
    Works when the slice has several disjoint contours and when some
    contours are surrounding the exterior of the mesh (holes inside
    the slice).
    
    Relatively optimized:
    - Once per spot: get all the Z slices. Involves iterating once through
    all the triangles, sorting 2 arrays and a few binary search.
    - Once per X line: compute intersections of a ray along the X axis
    with all the contours.
    - Once per pixel: binary search against intersection to know how
    many of them we crossed.
    tinevez committed Jul 22, 2024
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  56. Remove unused classes.

    tinevez committed Jul 22, 2024
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  57. Update mesh demos.

    tinevez committed Jul 22, 2024
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  58. Meshes are stored centered at (0,0,0)

    They and translated on the fly when iterating over pixels,
    saving and diplaying.
    tinevez committed Jul 22, 2024
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  59. Create a proper mask from the input before using the mask detector.

    A small routine builds a view of the input (that should be a mask)
    where all pixels greater than 0 are set to 1 and to 0 otherwise.
    In the case of meshes, this allows using the marching cube algorithm
    on real type, interpolating at 0.5 between 0 and 1, and having
    smoother meshes than when using the marching cube on boolean types.
    tinevez committed Jul 22, 2024
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  61. Mesh slices can be painted filled.

    Holes in slices are properly handled.
    But only in the case of contours generated by meshes that are
    manifold and two-manifold.
    tinevez committed Jul 22, 2024
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  62. Code style changes.

    tinevez committed Jul 22, 2024
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  63. Precompute and store a cache of Z-slices for meshes.

    We don't want to recompute them every time we have to paint the
    spot-meshes, or iterate through their pixel. And we have an
    optimized version of the ZSlicer that computes Z-slices in batch
    with several Zs. This makes the display and iteration in the
    TrackMate app much much faster for large meshes.
    
    The difficulty is that we need to specify a scale in XY and in
    Z (to simplify the  contours, and compute the Z positions of
    the intersections with the image stack slices). But we don't
    want to store these scales - which are the pixel size typically -
    in the model, that should be independent of a reference to an image.
    
    The solution in this commit consists in receiving these
    scales when we require the precomputed Z-slices: the cache is
    then recomputed if needed, and we have these scales in the code
    when we require the Z-slices
    tinevez committed Jul 22, 2024
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  65. Can manually scale meshes in size.

    Not sure it is useful, but we can do it for normal spots, rois
    and now meshes.
    tinevez committed Jul 22, 2024
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  67. Fix mistake with mesh cursor.

    tinevez committed Jul 22, 2024
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  70. Support making single spots with possibly hollow meshes.

    That is: we do not rely on connected components to separate mesh,
    but instead simply use the ImgLib2 regions.
    tinevez committed Jul 22, 2024
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  72. Update demo.

    tinevez committed Jul 22, 2024
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  74. Recompute the Z-slices when changing the radius.

    Nevermind the XY changes optimization.
    tinevez committed Jul 22, 2024
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  75. Rename 2D morphology analyzers.

    To make room for the 3D analyzers.
    tinevez committed Jul 22, 2024
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  76. Add flag for detectors that can return 3D shape,

    and interfaces for analyzers that can exploit it.
    tinevez committed Jul 22, 2024
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  77. Add ops as a dependency.

    tinevez committed Jul 22, 2024
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  78. Add VOLUME as a dimension.

    tinevez committed Jul 22, 2024
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  79. Add basic 3D shape analyzers.

    TODO: Ellipsoid fitting, boxity.
    tinevez committed Jul 22, 2024
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  80. Remove unused class.

    tinevez committed Jul 22, 2024
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  81. WIP: Ellipsoid fitter.

    Fit an ellipsoid to the convex-Hull of a 3D mesh.
    Adapted from Yury Petrov's EllipsoidFit MATLAB function
    and KalebKE ellipsoidfit (Apache license)
    TODO: Test!
    tinevez committed Jul 22, 2024
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  83. JUnit test for the ellipsoid fitter.

    Contains a routine to generate the mesh of an ellipsoid
    programmatically.
    tinevez committed Jul 22, 2024
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  86. Spot is now an interface, with 3 derived class.

    Spot -> the main interface, used by default in trackers. Define
    basic methods to get and store feature values.
    SpotBase -> Plain spots, like for TrackMate v<7
    SpotRoi -> spot has a polygon as a contour in 2D
    SpotMesh -> spot has a 3D mesh
    
    More elegant and extensible to app consuming TrackMate trackers
    with special objects.
    tinevez committed Jul 22, 2024
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  87. Fix some javadoc errors.

    tinevez committed Jul 22, 2024
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  89. Remove unused interfaces.

    tinevez committed Jul 22, 2024
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  92. Rework the SpotRoi class.

    - Make fields private, replace by public methods.
    - Review iteration over coordinates.
    - More sensible convenience methods.
    tinevez committed Jul 22, 2024
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  94. Rework the SpotMesh class.

    Simply make the mesh field private, accessible via a public method.
    tinevez committed Jul 22, 2024
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  95. WIP: An action to export all the meshes in a model to a PLY file series.

    So that they can be opened as a time series in ParaView.
    tinevez committed Jul 22, 2024
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  96. Fix loading of SpotMeshes.

    tinevez committed Jul 22, 2024
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  99. Fix meshh series exporter.

    tinevez committed Jul 22, 2024
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  100. Make a utility method public.

    tinevez committed Jul 22, 2024
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  101. Rework the 2D and 3D detection utils.

    We have now 3D meshes that we get via the marching cubes. This
    algorithm behaves slightly differently for grayscale + threshold
    and mask images, so we have to implement that in TrackMate.
    
    First: split the shape-related methods of MaskUtils in two
    utility classes SpotRoiUtils and SpotMeshUtils so as to avoid
    having a single gigantic class.
    
    With grayscale thresholded image, the marching cube algorithm can
    return nice, smooth, interpolated meshes, which is what we want
    in a biological context. So there is now in SpotMeshUtils a method
    that exploits this in the following manner:
    
    The grayscale marching-cube algorithm is used to create one big mesh
    from the source image. It is then split in connected-components to
    create single spot objects. However, to deal with possible holes
    in objects, meshes are possibly re-merged based on full inclusion
    of their bounding-box. For instance, a hollow sphere would be
    represented by two connected-components, yielding two meshes.
    But because the small one isincluded in the big one, they are
    merged in this method so that the hole is properly included in the
    shape representation.
    
    When dealing with masks as inputs, we want to generate a mesh that
    can retrieve the exact pixel content of the mask when iterated. So
    a separate method convert the mask into a label image, and each
    of its connected-component is treated separately to generate a mesh.
    We will use the grayscale marching-cube algorithm, using a threshold
    value of 0.5 on the bit-masks resulting from connected-component
    analysis of the label image generated from the mask.
    tinevez committed Jul 22, 2024
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  104. Two new icons.

    tinevez committed Jul 22, 2024
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  106. A UI tool to build a selection of feature analyzers and save it to disk.

    Later we will make the GUI sessions use the saved selection, so that
    users can choose what they want to compute or not.
    Might be handy for some features that are long to compute.
    Contrast and SNR I am looking at you. And actually you are deselected
    by default now.
    tinevez committed Jul 22, 2024
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  110. Also abides to user spot analyser selection after detection step.

    Because morphology analyzers (2D or 3D) are added only after detection
    step (they are added at this moment, because we need to know whether
    the detector can return the spot shape).
    tinevez committed Jul 22, 2024
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  112. Convenience class to run 2D+Z segmentation algos in 3D.

    A SpotDetector for 3D images that work by running a spot
    segmentation algorithm on 2D slices, and merging results
    using a tracker. This yield a label image that is then
    converted to 3D meshes using the LabelImageDetector.
    
    This is a convenience class, made to be used in
    specialized SpotDetectorFactory with specific choices
    of detector and merging strategy.
    tinevez committed Jul 22, 2024
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  113. Fix bug in analyzer selection.

    When using the configure(Settings) method, the morphology analyzers
    were not added to the Settings object, even when selected.
    tinevez committed Jul 22, 2024
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  114. The GUI honors the user selection when computing spot features.

    The spot feature analyzer factories are directly read from
    the user selection.
    tinevez committed Jul 22, 2024
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  115. Use again the custom 2D iteration routine for SpotRoi.

    I was using it before and trash it in the overhaul. But this one
    is about 30x faster. So let's put it back, with some adapting.
    Noticed by @Mini-Miette
    tinevez committed Jul 22, 2024
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  116. Tweak javadoc.

    tinevez committed Jul 22, 2024
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  118. Make sure the user selection of analyzers are added in priority order.

    This is crucial, as some analyzers depend on others to have been
    computed. This is implemented following the priority of the analyzer
    factories, which is known by the provider. The provider returns a list
    of keys, in priority order, so we must make sure to reproduce this
    order with the user selection.
    Hence the gymnastic in the configure(Settings) method.
    tinevez committed Jul 22, 2024
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  119. Implement quality measurement on the 3D object.

    Without using the mesh actually, we use the bitmask provided.
    tinevez committed Jul 22, 2024
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  120. Spot is now an interface, with 3 derived class.

    Spot -> the main interface, used by default in trackers. Define
    basic methods to get and store feature values.
    SpotBase -> Plain spots, like for TrackMate v<7
    SpotRoi -> spot has a polygon as a contour in 2D
    SpotMesh -> spot has a 3D mesh
    
    More elegant and extensible to app consuming TrackMate trackers
    with special objects.
    tinevez committed Jul 22, 2024
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  121. simplify TMUtils.rawWraps

    tinevez committed Jul 22, 2024
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  123. Add jogl deps.

    tinevez committed Jul 22, 2024
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  125. WIP: A new viewer for TrackMate based on the BVV.

    Adapting the demo from Tobias. This currently requires the 'mesh' branch
    in the bvv repo to work. Right now it can display the 3D image (possibly
    multi-channel) over time, along with the meshes in spots (if there is
    no mesh, nothing is shown).
    There is still so much to do but this is cool!
    tinevez committed Jul 22, 2024
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  126. Copy of Tobias 'StupidMesh'.

    Modified so that the color of individual meshes can be changed at
    runtime. We also add a white halo so that object contours are visible
    over the black background.
    tinevez committed Jul 22, 2024
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  127. The BVV view abides to the spot color settings.

    Can be changed live using TrackMate display settings panels.
    tinevez committed Jul 22, 2024
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  131. Fix OverlapTracker not working if the first frame was devoid of visib…

    …le spots.
    
    Noticed by Laura Xénard  @Mini-Miette
    Fix #260
    tinevez committed Jul 22, 2024
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  132. Put a test drive in a try/catch block so that we can get exceptions.

    One bug of my Eclipse installation results in not having exceptions
    shown in the console.
    tinevez committed Jul 22, 2024
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  133. Minor tweak of the demo.

    tinevez committed Jul 22, 2024
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  138. Tweak error messages.

    tinevez committed Jul 22, 2024
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  142. WIP: on Process2DZ, assign quality, work well with ROIs.

    Does not work yet, if the zmin is not 0.
    tinevez committed Jul 22, 2024
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  144. Simplify StupidMesh.

    Remove unneeded fields.
    tinevez committed Jul 22, 2024
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  151. Update to non-SNAPSHOT version of bigvolumeviewer.

    This version is deployed on maven.imagej.net.
    Tobias made a special branch for TrackMate, and the difference I
    could identify is the presence of a method that returns the camera
    view. See the diff in TrackMateBVV.
    tinevez committed Jul 22, 2024
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  152. WIP: Trying to implement transparency of meshes.

    Right now we don't "see through" the meshes. They simply get darker.
    tinevez committed Jul 22, 2024
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  153. Fix cast error

    ?! How did it get there unnoticed?
    tinevez committed Jul 22, 2024
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  154. Spot is now an interface, with 3 derived class.

    Spot -> the main interface, used by default in trackers. Define
    basic methods to get and store feature values.
    SpotBase -> Plain spots, like for TrackMate v<7
    SpotRoi -> spot has a polygon as a contour in 2D
    SpotMesh -> spot has a 3D mesh
    
    More elegant and extensible to app consuming TrackMate trackers
    with special objects.
    tinevez committed Jul 22, 2024
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  155. Rework the 2D and 3D detection utils.

    We have now 3D meshes that we get via the marching cubes. This
    algorithm behaves slightly differently for grayscale + threshold
    and mask images, so we have to implement that in TrackMate.
    
    First: split the shape-related methods of MaskUtils in two
    utility classes SpotRoiUtils and SpotMeshUtils so as to avoid
    having a single gigantic class.
    
    With grayscale thresholded image, the marching cube algorithm can
    return nice, smooth, interpolated meshes, which is what we want
    in a biological context. So there is now in SpotMeshUtils a method
    that exploits this in the following manner:
    
    The grayscale marching-cube algorithm is used to create one big mesh
    from the source image. It is then split in connected-components to
    create single spot objects. However, to deal with possible holes
    in objects, meshes are possibly re-merged based on full inclusion
    of their bounding-box. For instance, a hollow sphere would be
    represented by two connected-components, yielding two meshes.
    But because the small one isincluded in the big one, they are
    merged in this method so that the hole is properly included in the
    shape representation.
    
    When dealing with masks as inputs, we want to generate a mesh that
    can retrieve the exact pixel content of the mask when iterated. So
    a separate method convert the mask into a label image, and each
    of its connected-component is treated separately to generate a mesh.
    We will use the grayscale marching-cube algorithm, using a threshold
    value of 0.5 on the bit-masks resulting from connected-component
    analysis of the label image generated from the mask.
    tinevez committed Jul 22, 2024
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  157. Depend on imglib2-mesh preview, not on net-imagej-mesh.

    Still a WIP: several utility methods in TrackMate about meshes are now
    in the imglib2-mesh dep.
    tinevez committed Jul 22, 2024
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  158. Use the scale method of the spot interface.

    We don't have SpotShape anymore.
    tinevez committed Jul 22, 2024
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  160. Try to do without deprecated method.

    Given the message, we might see an error depending on the
    java JRE that runs. Using the Zulu one, this passes.
    tinevez committed Jul 22, 2024
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  161. Remove debug main method.

    tinevez committed Jul 22, 2024
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  164. Fix all javadoc warnings.

    tinevez committed Jul 22, 2024
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  166. Make the mesh of a SpotMesh setable.

    Also stop exposing the bounding-box.
    tinevez committed Jul 22, 2024
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  167. Return the actual BufferMesh class when we expose the mesh of a SpotM…

    …esh.
    
    TODO: use this in dependent methods.
    tinevez committed Jul 22, 2024
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  171. Improve smoothing action.

    - Actually run the tasks in multithreaded fashion (forgot to
    shutdown the executor service).
    - Notify the views that the spots have changed, including the 3D
    view. Takes a little delay but it's fine.
    tinevez committed Jul 22, 2024
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  176. Add a 'basic' setting panel to the mesh smoother.

    It filters with mu = 1., lambda -0, which is like applying heavy
    Laplace smoothing.
    tinevez committed Jul 22, 2024
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  178. WIP: Smooth mask or label before generating mesh to smooth it.

    Incomplete: miss the 2D case.
    tinevez committed Jul 22, 2024
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  180. Optionally check for the presence of a parameter in a settings map.

    Does not return an error if the parameter is not there, but
    if it is, checks that it is of the expected class.
    tinevez committed Jul 22, 2024
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  181. Tweak the slider panels.

    - Mouse wheel listener.
    - Method to dis/enable all sub component.
    tinevez committed Jul 22, 2024
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  182. fix the PanelSmoothContour.

    tinevez committed Jul 22, 2024
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  183. Implement smoothing of images before segmentation.

    Changes in the core methods in MaskUtils, SpotMeshUtils and
    SpotRoiUtils:
    
    - They take a smoothingScale double parameter.
    
    - If negative, it is ignored, and the segmentation (by mask, threshold
    and label image) processes directly on the input image.
    
    - Otherwise, the inputs are filtered by a Gaussian filter with sigmas
    derived from the specified scale. In 2D and 3D. Even for the bitmasks
    of the label image. This yields much smoother meshes and contour,
    of a smoothness controlled by the scale.
    
    - This is different from the 'simplify' flag, that prunes the contour
    and the mesh, but still 'sticks' to the shape in the input without
    filtering.
    tinevez committed Jul 22, 2024
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  186. Fix incorrect documentation.

    tinevez committed Jul 22, 2024
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  188. Move the shader files to the resources folder.

    So that they are properly included when compiling with maven.
    tinevez committed Jul 22, 2024
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  192. Add a button to launch LabKit in the configure views panel of the wiz…

    …ard.
    
    But because the other table, trackscheme and bvv buttons take
    too much place, we don't see it without resizing the window.
    tinevez committed Jul 22, 2024
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  193. Rework and improve the import method of the spot editor.

    - We don't depend on labels anymore, but directly operate and compare
    the index images (before modification and after). Because the index
    is directly related to the spot ID, we can get a match from previous
    spot to novel spot in an easy manner.
    
    - The spots from the edited version are created directly from the
    novel index image, using something adapted from the label image
    detector code, so again, just one pass. We use the fact that we can
    provide it with a 'quality' image, and read the index of the label
    image 'under' the spot and write it into its quality value.
    This way we can retrieve the id of the matching previous
    spot in an easy manner.
    
    - The price to pay for not working with labels anymore
    is that we don't have access to the label name, but that's life.
    
    - We make only one pass over the image to collect the ids of the spots
    that have been modified, instead of one pass per spot. Also, this
    pass is multithreaded (thanks LoopBuilder).
    
    - I have also learned that I should not use weakListeners() if I
    am doing something with threads inside the listener. Using listeners()
    instead works, but I do not know why the other one does not.
    Probably something arcane with Java WeakReferences being collected.
    
    - As a result of all this the performance is much better than before
    and the 'return to TrackMate' should happen without the user noticing
    the process.
    tinevez committed Jul 22, 2024
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  194. Try to fix LegacyService error.

    This test fails because of the LegacyService, see the stack trace
    below.
    
    If I add the
    
    static
    {
    	net.imagej.patcher.LegacyInjector.preinit();
    }
    
    initializer block as suggested here:
    https://forum.image.sc/t/imagej-legacy-error/23013
    
    then the test passes in Eclipse, but still fails in Maven.
    
    So this commit at least adds the initializer so that it works
    in Eclipse. Note that it still fails on Maven, and proably
    with deploy actions too.
    
    
    
    
    [INFO] Running fiji.plugin.trackmate.TrackMatePluginTest
    [ERROR] Tests run: 1, Failures: 0, Errors: 1, Skipped: 0, Time elapsed: 0.787 s <<< FAILURE! -- in fiji.plugin.trackmate.TrackMatePluginTest
    [ERROR] fiji.plugin.trackmate.TrackMatePluginTest.testTrackMateRegistration -- Time elapsed: 0.787 s <<< ERROR!
    java.lang.ExceptionInInitializerError
    	at sun.reflect.NativeConstructorAccessorImpl.newInstance0(Native Method)
    	at sun.reflect.NativeConstructorAccessorImpl.newInstance(NativeConstructorAccessorImpl.java:62)
    	at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:45)
    	at java.lang.reflect.Constructor.newInstance(Constructor.java:423)
    	at org.junit.runners.BlockJUnit4ClassRunner.createTest(BlockJUnit4ClassRunner.java:250)
    	at org.junit.runners.BlockJUnit4ClassRunner.createTest(BlockJUnit4ClassRunner.java:260)
    	at org.junit.runners.BlockJUnit4ClassRunner$2.runReflectiveCall(BlockJUnit4ClassRunner.java:309)
    	at org.junit.internal.runners.model.ReflectiveCallable.run(ReflectiveCallable.java:12)
    	at org.junit.runners.BlockJUnit4ClassRunner.methodBlock(BlockJUnit4ClassRunner.java:306)
    	at org.junit.runners.BlockJUnit4ClassRunner$1.evaluate(BlockJUnit4ClassRunner.java:100)
    	at org.junit.runners.ParentRunner.runLeaf(ParentRunner.java:366)
    	at org.junit.runners.BlockJUnit4ClassRunner.runChild(BlockJUnit4ClassRunner.java:103)
    	at org.junit.runners.BlockJUnit4ClassRunner.runChild(BlockJUnit4ClassRunner.java:63)
    	at org.junit.runners.ParentRunner$4.run(ParentRunner.java:331)
    	at org.junit.runners.ParentRunner$1.schedule(ParentRunner.java:79)
    	at org.junit.runners.ParentRunner.runChildren(ParentRunner.java:329)
    	at org.junit.runners.ParentRunner.access$100(ParentRunner.java:66)
    	at org.junit.runners.ParentRunner$2.evaluate(ParentRunner.java:293)
    	at org.junit.runners.ParentRunner$3.evaluate(ParentRunner.java:306)
    	at org.junit.runners.ParentRunner.run(ParentRunner.java:413)
    	at org.apache.maven.surefire.junit4.JUnit4Provider.execute(JUnit4Provider.java:316)
    	at org.apache.maven.surefire.junit4.JUnit4Provider.executeWithRerun(JUnit4Provider.java:240)
    	at org.apache.maven.surefire.junit4.JUnit4Provider.executeTestSet(JUnit4Provider.java:214)
    	at org.apache.maven.surefire.junit4.JUnit4Provider.invoke(JUnit4Provider.java:155)
    	at org.apache.maven.surefire.booter.ForkedBooter.runSuitesInProcess(ForkedBooter.java:385)
    	at org.apache.maven.surefire.booter.ForkedBooter.execute(ForkedBooter.java:162)
    	at org.apache.maven.surefire.booter.ForkedBooter.run(ForkedBooter.java:507)
    	at org.apache.maven.surefire.booter.ForkedBooter.main(ForkedBooter.java:495)
    Caused by: java.lang.RuntimeException: Found incompatible ImageJ class
    	at net.imagej.patcher.LegacyEnvironment.initialize(LegacyEnvironment.java:112)
    	at net.imagej.patcher.LegacyEnvironment.applyPatches(LegacyEnvironment.java:494)
    	at net.imagej.patcher.LegacyInjector.preinit(LegacyInjector.java:400)
    	at net.imagej.patcher.LegacyInjector.preinit(LegacyInjector.java:379)
    	at fiji.plugin.trackmate.TrackMatePluginTest.<clinit>(TrackMatePluginTest.java:40)
    	... 28 more
    Caused by: java.lang.RuntimeException: Cannot load class: ij.gui.ImageWindow (loader: sun.misc.Launcher$AppClassLoader@18b4aac2)
    It appears that this class was already defined in the class loader!
    Please make sure that you initialize the LegacyService before using
    any ImageJ 1.x class. You can do that by adding this static initializer:
    
    	static {
    		LegacyInjector.preinit();
    	}
    
    To debug this issue, start the JVM with the option:
    
    	-javaagent:/Users/tinevez/.m2/repository/net/imagej/ij1-patcher/1.2.6/ij1-patcher-1.2.6.jar
    
    To enforce pre-initialization, start the JVM with the option:
    
    	-javaagent:/Users/tinevez/.m2/repository/net/imagej/ij1-patcher/1.2.6/ij1-patcher-1.2.6.jar=init
    
    	at net.imagej.patcher.CodeHacker.javaAgentHint(CodeHacker.java:826)
    	at net.imagej.patcher.CodeHacker.loadClass(CodeHacker.java:805)
    	at net.imagej.patcher.CodeHacker.loadClasses(CodeHacker.java:853)
    	at net.imagej.patcher.LegacyInjector.injectHooks(LegacyInjector.java:114)
    	at net.imagej.patcher.LegacyEnvironment.initialize(LegacyEnvironment.java:100)
    	... 32 more
    Caused by: java.lang.ClassFormatError: loader (instance of  sun/misc/Launcher$AppClassLoader): attempted  duplicate class definition for name: "ij/gui/ImageWindow"
    	at javassist.util.proxy.DefineClassHelper$Java7.defineClass(DefineClassHelper.java:182)
    	at javassist.util.proxy.DefineClassHelper.toClass(DefineClassHelper.java:260)
    	at javassist.ClassPool.toClass(ClassPool.java:1240)
    	at javassist.CtClass.toClass(CtClass.java:1392)
    	at net.imagej.patcher.CodeHacker.loadClass(CodeHacker.java:799)
    	... 35 more
    tinevez committed Jul 22, 2024
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  195. Fix reimporting labels with large IDs in 2D.

    The re-importing of labels from Tabkit to TrackMate could fail for
    2D images and labels with a large index. For instance, it failed
    consistently when trying to re-import labels with an index larger
    than 65643.
    
    This problem roots in the getSpots() method of LabkitImporter. It
    relies on a trick: We get the new label image, and create spots from
    this label image. But we want the new spots to keep track of the index
    in the label image they were generated from.
    
    For this, in 2D, we use the SpotRoiUtils.from2DLabelingWithRoi()
    method. These methods accept an image as last argument used to
    read a value in the label image within the spot, that is normally
    used for the quality value of the new spot.
    
    But the SpotRoiUtils.from2DLabelingWithRoi() method converted the
    extra image to ImagePlus (because I was lazy). So the label image
    was effectively cast on ushort for an IntegerType image, hence
    the problem with the max label being 65453.
    
    The solution is to rewrite the from2DLabelingWithRoi() so that
    it does not rely on converting to ImagePlus, but on good old
    iteration with imglib2.
    tinevez committed Jul 22, 2024
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  197. Fix the legacy error issue?

    Moving the plugin implementation out of the test class and
    removing the legacy injector make the test pass in maven.
    tinevez committed Jul 22, 2024
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  198. Configuration menu
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  199. All labeling methods now use the double[] origin to reposition spots.

    Instead of the interval, so that this is consistent across
    TrackMate.
    tinevez committed Jul 22, 2024
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  200. Update license blurbs.

    tinevez committed Jul 22, 2024
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  201. Temporarily disable enforcer checks.

    For the beta phase.
    tinevez committed Jul 22, 2024
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  202. Configuration menu
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  203. Keep imagej-legacy out of the dependency tree

    This works around a test failure in TrackMatePluginTest caused by the
    original ImageJ classes being loaded too soon. There are other ways of
    working around this problem, such as creating a SciJava context more
    eagerly, but it's a distraction from the business of testing TrackMate,
    and anyway we don't need imagej-legacy on the classpath.
    
    The only reason labkit-ui has imagej-legacy as a dependency is for one
    line of code, which can be refactored to avoid it, so hopefully the
    exclusion will be able to disappear later after labkit-ui is updated.
    ctrueden authored and tinevez committed Jul 22, 2024
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  204. POM: fix the dependencies

    * Avoid SNAPSHOT versions.
    * Factor out version pins to properties.
    * Avoid jogamp *-main uber-JARs.
    ctrueden authored and tinevez committed Jul 22, 2024
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  205. Fix Javadoc errors

    ctrueden authored and tinevez committed Jul 22, 2024
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  206. Configuration menu
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  207. Fix minor Eclipse warnings.

    tinevez committed Jul 22, 2024
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  208. Configuration menu
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  209. Clamp slider widget values.

    tinevez committed Jul 22, 2024
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  211. Add required methods in SpotRoiUtils.

    We want to retrieve spots as a map feom labels to corresponding
    spots.
    tinevez committed Jul 22, 2024
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  214. Fixed one more bug with the labkit importer.

    When editing the whole movie, if the label of a new spot was using
    the label of an existing spot in another time-point, the existing
    one was removed. Because the new spot was identified as a modification
    of the existing one.
    The solution is to pass to the importer only the list of existing
    spots in the current time-frame.
    tinevez committed Jul 22, 2024
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Commits on Jul 23, 2024

  1. Add a methods to return the map of label to spots in a labeling image.

    Counterpart to the same one in SpotRoiUtils.
    Nota: the methods signature order should be harmonized between the
    two utility classes.
    tinevez committed Jul 23, 2024
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  2. Support editing in 3D in the LabKit editor.

    There is one discrepancy with the 2D part, linked to TrackMate:
    In 2D, one connected components will give one spot, even if several
    connected components belong to the same label.
    In 3D, it is possible to have one spot made of several disconnected
    components. Will have to document this.
    tinevez committed Jul 23, 2024
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  3. Minor typo.

    tinevez committed Jul 23, 2024
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  4. Add the smoothing scale param to the 2D labeling methods as well.

    Not implemented yet, but at least symmetric with the 2D case.
    tinevez committed Jul 23, 2024
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  5. Remove unused methods.

    tinevez committed Jul 23, 2024
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