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This repository has been archived by the owner on Apr 19, 2023. It is now read-only.
Describe the bug
Applying the scanpynormalize_per_cell function 2 times on the same expression matrix (containing 10x data and RPK SMART-seq2 data) lead to 2 different but very similar results. This bug has also been described more thoroughly here: scverse/scanpy#1612.
nextflow -C nextflow.config run vibsinglecellnf/vsn-pipelines -entry harmony
See error:
No log error. The problem is that the X slot in the AnnData is not reproducible after the normalization step normalize_per_cell between 2 runs. This leads to minor differences into downstream steps (embeddings, ...)
Expected behavior
Both should equal. Interestingly, this seems to happen for smaller datasets like PBMC 1k, 3k datasets from 10x.
Screenshots
If applicable, add screenshots to help explain your problem.
Please complete the following information:
OS: CentOS
Nextflow Version: 20.11.0-edge.5448
vsn-pipelines Version: 0.25.0
Additional context
Add any other context about the problem here.
The text was updated successfully, but these errors were encountered:
dweemx
changed the title
[BUG] normalize_per_cell not reproducible with np.float32
[BUG] normalize_per_cell can lead to not exact reproducible results with mixed data
Jan 27, 2021
Describe the bug
Applying the
scanpy
normalize_per_cell
function 2 times on the same expression matrix (containing 10x data and RPK SMART-seq2 data) lead to 2 different but very similar results. This bug has also been described more thoroughly here: scverse/scanpy#1612.To Reproduce
Steps to reproduce the behavior:
No log error. The problem is that the
X
slot in the AnnData is not reproducible after the normalization stepnormalize_per_cell
between 2 runs. This leads to minor differences into downstream steps (embeddings, ...)Expected behavior
Both should equal. Interestingly, this seems to happen for smaller datasets like PBMC 1k, 3k datasets from 10x.
Screenshots
If applicable, add screenshots to help explain your problem.
Please complete the following information:
Additional context
Add any other context about the problem here.
The text was updated successfully, but these errors were encountered: