E. coli 20kb Size Selected Library with P4 C2

Instrument:  PacBio RS II
Chemistry:  C2
Enzyme: P4

Summary

This dataset includes one SMRT Cell of data gathered with a PacBio RS II System and P4-C2 chemistry on a size selected 20kb library of E. coli K12. Assembling this SMRT Cell results in a single contig at >Q50 accuracy using SMRT Analysis 2.0 or higher.

Modification and Motif analysis may be performed on using the same data as a resequencing job (i.e., against a publicly available K12 reference genome or against a reference created by the de novo assembly) which will find the two 6-mA methyltransferase recognition motifs (one of which is non-palindromic).

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Primary Analysis (Instrument Output) Data

• Input SMRT Cell data files (6.35 GB) including metadata.xml, bas.h5, and bax.h5 files that can be imported into SMRT Analysis.

Secondary Analysis Data

• Final genome assembly in FASTA format (4.7 MB). HGAP was run using default parameters in SMRT Portal.