H. sapiens 10x Sequence Coverage with PacBio data

Instrument:  PacBio RS II
Chemistry:  C3
Enzyme: P5

Summary

The dataset released in this directory contains the raw sequence data resulting from PacBio® SMRT® Sequencing for CHM1TERT, a human cell line derived from a hydatidiform mole, as a resource for general community exploration. Two shotgun libraries were prepared from the same DNA sample, with average insert sizes of ~20 and ~30 KB, respectively. Size selection was performed using 7.5 KB and 10 KB elution cutoffs, respectively, on a BluePippin™ DNA size-selection system from SAGE Science. The genome was sequenced using P5-C3 chemistry and 3-hour SMRT Cell acquisitions to generate ~32 GB of sequence data.

Sequencing Data Statistics
Total number of reads: 3,679,463
Total number of post-filtered bases: 32,559,803,198

Read length statistics		
Half of sequenced bases in reads greater than: 10,985 bp
5% of reads longer than: 19,060 bp

SMRTbell template statistics
Longest DNA insert sequenced: 41,460 bp
5% of sequenced DNA inserts longer than: 18,060 bp
Average sequenced DNA insert length: 7,406 bp
	
PacBio RS II instrument time for sequencing: 10 days
Number of SMRT Cells: 66

Download Dataset

To access the dataset, please send an email to pbdata@pacificbiosciences.com. You will receive an automated email response with a link to the dataset.

To download, you can use wget or curl to go through the list of the file. For example, to download it with bash, save the list as file file_list and you can use a simple loop to download the files: for f in cat file_list;do wget $f;done. The raw data is available upon request. Please contact us, or convey your interest via twitter @PacBio. We appreciate if you could follow us on twitter so that we can direct message in response.